Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/32393 http://dx.doi.org/10.1210/jc.2009-1354 |
Resumo: | Context: Serum thyroglobulin is a sensitive tumor marker in the follow-up of patients with differentiated thyroid carcinoma (DTC), but the presence of endogenous anti-thyroglobulin antibodies (TgAb) can interfere on its measurement. To prevent interference by TgAb, several investigators have tried to quantify blood mRNA Tg by real-time RT-PCR, but the results have been variable, not reporting a correlation between mRNA Tg and the presence of metastases.Objective: the aim of the study was to evaluate the development of a sensitive and specific quantitative RT-PCR assay for blood mRNA Tg in the follow-up of patients with DTC.Design and Patients: An assay employing primers located in a region not affected by alternative splicing or single nucleotide polymorphisms was developed to study 104 DTC patients (13 of 104 with positive TgAb).Results: the assay is specific for thyroid tissue because we found mRNA Tg expression in normal thyroid tissue, but we did not find any mRNA Tg expression in any extrathyroidal tissues. Quantitative mRNA Tg levels were significantly different between patients free of disease (82 of 104) and those with metastases (22 of 104) (2.61 +/- 0.26 vs. 27.58 +/- 1.62 pg mRNA Tg/mu g RNA) (P < 0.0001). A cutoff point of 5.51 was able to discriminate between the two groups. in addition, the measurement of mRNA Tg was not affected by the presence of TgAb.Conclusion: This new mRNA Tg quantification is a reliable method that allowed us to differentiate patients free of disease from those with metastases, and it could represent an appropriate molecular marker for the follow-up of patients with DTC, especially those with positive TgAb. (J Clin Endocrinol Metab 95: 1726-1733, 2010) |
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Boldarine, Valter Tadeu [UNIFESP]Maciel, Rui Monteiro de Barros [UNIFESP]Guimaraes, Gustavo Stuani [UNIFESP]Nakabashi, Claudia Cristina Doimo [UNIFESP]Camacho, Cléber Pinto [UNIFESP]Andreoni, Danielle Macellaro [UNIFESP]Mamone, Maria da Conceição de Oliveira Carneiro [UNIFESP]Ikejiri, Elza Setsuko [UNIFESP]Kasamatsu, Teresa Sayoko [UNIFESP]Crispim, Felipe [UNIFESP]Hojaij, Flavio Carneiro [UNIFESP]Hidal, Jairo Tabacow [UNIFESP]Biscolla, Rosa Paula Mello [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Inst Israelita Ensino & Pesquisa Albert EinsteinFleury Med & Hlth2016-01-24T13:59:29Z2016-01-24T13:59:29Z2010-04-01Journal of Clinical Endocrinology & Metabolism. Chevy Chase: Endocrine Soc, v. 95, n. 4, p. 1726-1733, 2010.0021-972Xhttp://repositorio.unifesp.br/handle/11600/32393http://dx.doi.org/10.1210/jc.2009-135410.1210/jc.2009-1354WOS:000276402300030Context: Serum thyroglobulin is a sensitive tumor marker in the follow-up of patients with differentiated thyroid carcinoma (DTC), but the presence of endogenous anti-thyroglobulin antibodies (TgAb) can interfere on its measurement. To prevent interference by TgAb, several investigators have tried to quantify blood mRNA Tg by real-time RT-PCR, but the results have been variable, not reporting a correlation between mRNA Tg and the presence of metastases.Objective: the aim of the study was to evaluate the development of a sensitive and specific quantitative RT-PCR assay for blood mRNA Tg in the follow-up of patients with DTC.Design and Patients: An assay employing primers located in a region not affected by alternative splicing or single nucleotide polymorphisms was developed to study 104 DTC patients (13 of 104 with positive TgAb).Results: the assay is specific for thyroid tissue because we found mRNA Tg expression in normal thyroid tissue, but we did not find any mRNA Tg expression in any extrathyroidal tissues. Quantitative mRNA Tg levels were significantly different between patients free of disease (82 of 104) and those with metastases (22 of 104) (2.61 +/- 0.26 vs. 27.58 +/- 1.62 pg mRNA Tg/mu g RNA) (P < 0.0001). A cutoff point of 5.51 was able to discriminate between the two groups. in addition, the measurement of mRNA Tg was not affected by the presence of TgAb.Conclusion: This new mRNA Tg quantification is a reliable method that allowed us to differentiate patients free of disease from those with metastases, and it could represent an appropriate molecular marker for the follow-up of patients with DTC, especially those with positive TgAb. (J Clin Endocrinol Metab 95: 1726-1733, 2010)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Research-Fellowship GrantUniversidade Federal de São Paulo, Dept Med, Escola Paulista Med, Div Endocrinol,Lab Mol Endocrinol, BR-04039032 São Paulo, BrazilInst Israelita Ensino & Pesquisa Albert Einstein, Thyroid Dis Ctr, BR-05652000 São Paulo, BrazilFleury Med & Hlth, BR-01243001 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Escola Paulista Med, Div Endocrinol,Lab Mol Endocrinol, BR-04039032 São Paulo, BrazilFAPESP: 04/09934-7Research-Fellowship Grant: 05/55842-0Web of Science1726-1733engEndocrine SocJournal of Clinical Endocrinology & MetabolismDevelopment of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinomainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/323932023-02-15 09:10:19.131metadata only accessoai:repositorio.unifesp.br:11600/32393Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T12:10:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
title |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
spellingShingle |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma Boldarine, Valter Tadeu [UNIFESP] |
title_short |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
title_full |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
title_fullStr |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
title_full_unstemmed |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
title_sort |
Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma |
author |
Boldarine, Valter Tadeu [UNIFESP] |
author_facet |
Boldarine, Valter Tadeu [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Guimaraes, Gustavo Stuani [UNIFESP] Nakabashi, Claudia Cristina Doimo [UNIFESP] Camacho, Cléber Pinto [UNIFESP] Andreoni, Danielle Macellaro [UNIFESP] Mamone, Maria da Conceição de Oliveira Carneiro [UNIFESP] Ikejiri, Elza Setsuko [UNIFESP] Kasamatsu, Teresa Sayoko [UNIFESP] Crispim, Felipe [UNIFESP] Hojaij, Flavio Carneiro [UNIFESP] Hidal, Jairo Tabacow [UNIFESP] Biscolla, Rosa Paula Mello [UNIFESP] |
author_role |
author |
author2 |
Maciel, Rui Monteiro de Barros [UNIFESP] Guimaraes, Gustavo Stuani [UNIFESP] Nakabashi, Claudia Cristina Doimo [UNIFESP] Camacho, Cléber Pinto [UNIFESP] Andreoni, Danielle Macellaro [UNIFESP] Mamone, Maria da Conceição de Oliveira Carneiro [UNIFESP] Ikejiri, Elza Setsuko [UNIFESP] Kasamatsu, Teresa Sayoko [UNIFESP] Crispim, Felipe [UNIFESP] Hojaij, Flavio Carneiro [UNIFESP] Hidal, Jairo Tabacow [UNIFESP] Biscolla, Rosa Paula Mello [UNIFESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Inst Israelita Ensino & Pesquisa Albert Einstein Fleury Med & Hlth |
dc.contributor.author.fl_str_mv |
Boldarine, Valter Tadeu [UNIFESP] Maciel, Rui Monteiro de Barros [UNIFESP] Guimaraes, Gustavo Stuani [UNIFESP] Nakabashi, Claudia Cristina Doimo [UNIFESP] Camacho, Cléber Pinto [UNIFESP] Andreoni, Danielle Macellaro [UNIFESP] Mamone, Maria da Conceição de Oliveira Carneiro [UNIFESP] Ikejiri, Elza Setsuko [UNIFESP] Kasamatsu, Teresa Sayoko [UNIFESP] Crispim, Felipe [UNIFESP] Hojaij, Flavio Carneiro [UNIFESP] Hidal, Jairo Tabacow [UNIFESP] Biscolla, Rosa Paula Mello [UNIFESP] |
description |
Context: Serum thyroglobulin is a sensitive tumor marker in the follow-up of patients with differentiated thyroid carcinoma (DTC), but the presence of endogenous anti-thyroglobulin antibodies (TgAb) can interfere on its measurement. To prevent interference by TgAb, several investigators have tried to quantify blood mRNA Tg by real-time RT-PCR, but the results have been variable, not reporting a correlation between mRNA Tg and the presence of metastases.Objective: the aim of the study was to evaluate the development of a sensitive and specific quantitative RT-PCR assay for blood mRNA Tg in the follow-up of patients with DTC.Design and Patients: An assay employing primers located in a region not affected by alternative splicing or single nucleotide polymorphisms was developed to study 104 DTC patients (13 of 104 with positive TgAb).Results: the assay is specific for thyroid tissue because we found mRNA Tg expression in normal thyroid tissue, but we did not find any mRNA Tg expression in any extrathyroidal tissues. Quantitative mRNA Tg levels were significantly different between patients free of disease (82 of 104) and those with metastases (22 of 104) (2.61 +/- 0.26 vs. 27.58 +/- 1.62 pg mRNA Tg/mu g RNA) (P < 0.0001). A cutoff point of 5.51 was able to discriminate between the two groups. in addition, the measurement of mRNA Tg was not affected by the presence of TgAb.Conclusion: This new mRNA Tg quantification is a reliable method that allowed us to differentiate patients free of disease from those with metastases, and it could represent an appropriate molecular marker for the follow-up of patients with DTC, especially those with positive TgAb. (J Clin Endocrinol Metab 95: 1726-1733, 2010) |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-04-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:59:29Z |
dc.date.available.fl_str_mv |
2016-01-24T13:59:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Clinical Endocrinology & Metabolism. Chevy Chase: Endocrine Soc, v. 95, n. 4, p. 1726-1733, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/32393 http://dx.doi.org/10.1210/jc.2009-1354 |
dc.identifier.issn.none.fl_str_mv |
0021-972X |
dc.identifier.doi.none.fl_str_mv |
10.1210/jc.2009-1354 |
dc.identifier.wos.none.fl_str_mv |
WOS:000276402300030 |
identifier_str_mv |
Journal of Clinical Endocrinology & Metabolism. Chevy Chase: Endocrine Soc, v. 95, n. 4, p. 1726-1733, 2010. 0021-972X 10.1210/jc.2009-1354 WOS:000276402300030 |
url |
http://repositorio.unifesp.br/handle/11600/32393 http://dx.doi.org/10.1210/jc.2009-1354 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Clinical Endocrinology & Metabolism |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1726-1733 |
dc.publisher.none.fl_str_mv |
Endocrine Soc |
publisher.none.fl_str_mv |
Endocrine Soc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764191473336320 |