Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/44369 http://dx.doi.org/10.1099/00221287-139-7-1543 |
Resumo: | As tuberculosis re-emerges as an important health problem worldwide, new drugs, better diagnostic tests and vaccines are being sought. In order to identify potentially useful peptides for the development of a synthetic vaccine against tuberculosis, immunological and functional studies were performed using proteins of the antigen 85 complex. Western blot (immuno-blot) analysis and a lymphoproliferation study was used to investigate the B- and T-cell immune response of tuberculosis patients, healthy household contacts and normal controls to proteins of the Mycobacterium tuberculosis antigen 85 complex. Peptides derived from the 85A amino acid sequence were synthesized and used in fibronectin-binding and in ELISA assays. A peptide with the sequence CQPACRKAGCQTYKWEC bound to radiolabelled fibronectin in a time-dependent manner and was recognized by human sera in ELISA. This peptide was identified as a potential component of a synthetic vaccine against tuberculosis. |
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Leao, S. C.Lopes, Jose Daniel [UNIFESP]Patarroyo, M. E.Universidade Federal de São Paulo (UNIFESP)2018-06-15T17:58:47Z2018-06-15T17:58:47Z1993-07-01Journal Of General Microbiology. Reading: Soc General Microbiology, v. 139, n. 7, p. 1543-1549, 1993.0022-1287http://repositorio.unifesp.br/11600/44369http://dx.doi.org/10.1099/00221287-139-7-154310.1099/00221287-139-7-1543WOS:A1993LN97900018As tuberculosis re-emerges as an important health problem worldwide, new drugs, better diagnostic tests and vaccines are being sought. In order to identify potentially useful peptides for the development of a synthetic vaccine against tuberculosis, immunological and functional studies were performed using proteins of the antigen 85 complex. Western blot (immuno-blot) analysis and a lymphoproliferation study was used to investigate the B- and T-cell immune response of tuberculosis patients, healthy household contacts and normal controls to proteins of the Mycobacterium tuberculosis antigen 85 complex. Peptides derived from the 85A amino acid sequence were synthesized and used in fibronectin-binding and in ELISA assays. A peptide with the sequence CQPACRKAGCQTYKWEC bound to radiolabelled fibronectin in a time-dependent manner and was recognized by human sera in ELISA. This peptide was identified as a potential component of a synthetic vaccine against tuberculosis.ESCOLA PAULISTA MED, DISCIPLINA BIOL CELULAR, BR-04023-062 SAO PAULO, BRAZILESCOLA PAULISTA MED, DISCIPLINA BIOL CELULAR, BR-04023-062 SAO PAULO, BRAZILWeb of Science1543-1549engSoc General MicrobiologyJournal Of General MicrobiologyImmunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/443692022-02-08 11:52:11.638metadata only accessoai:repositorio.unifesp.br:11600/44369Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T14:52:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
title |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
spellingShingle |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides Leao, S. C. |
title_short |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
title_full |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
title_fullStr |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
title_full_unstemmed |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
title_sort |
Immunological and functional characterization of proteins of the Mycobacterium tuberculosis antigen 85 complex using synthetic peptides |
author |
Leao, S. C. |
author_facet |
Leao, S. C. Lopes, Jose Daniel [UNIFESP] Patarroyo, M. E. |
author_role |
author |
author2 |
Lopes, Jose Daniel [UNIFESP] Patarroyo, M. E. |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Leao, S. C. Lopes, Jose Daniel [UNIFESP] Patarroyo, M. E. |
description |
As tuberculosis re-emerges as an important health problem worldwide, new drugs, better diagnostic tests and vaccines are being sought. In order to identify potentially useful peptides for the development of a synthetic vaccine against tuberculosis, immunological and functional studies were performed using proteins of the antigen 85 complex. Western blot (immuno-blot) analysis and a lymphoproliferation study was used to investigate the B- and T-cell immune response of tuberculosis patients, healthy household contacts and normal controls to proteins of the Mycobacterium tuberculosis antigen 85 complex. Peptides derived from the 85A amino acid sequence were synthesized and used in fibronectin-binding and in ELISA assays. A peptide with the sequence CQPACRKAGCQTYKWEC bound to radiolabelled fibronectin in a time-dependent manner and was recognized by human sera in ELISA. This peptide was identified as a potential component of a synthetic vaccine against tuberculosis. |
publishDate |
1993 |
dc.date.issued.fl_str_mv |
1993-07-01 |
dc.date.accessioned.fl_str_mv |
2018-06-15T17:58:47Z |
dc.date.available.fl_str_mv |
2018-06-15T17:58:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal Of General Microbiology. Reading: Soc General Microbiology, v. 139, n. 7, p. 1543-1549, 1993. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/44369 http://dx.doi.org/10.1099/00221287-139-7-1543 |
dc.identifier.issn.none.fl_str_mv |
0022-1287 |
dc.identifier.doi.none.fl_str_mv |
10.1099/00221287-139-7-1543 |
dc.identifier.wos.none.fl_str_mv |
WOS:A1993LN97900018 |
identifier_str_mv |
Journal Of General Microbiology. Reading: Soc General Microbiology, v. 139, n. 7, p. 1543-1549, 1993. 0022-1287 10.1099/00221287-139-7-1543 WOS:A1993LN97900018 |
url |
http://repositorio.unifesp.br/11600/44369 http://dx.doi.org/10.1099/00221287-139-7-1543 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal Of General Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1543-1549 |
dc.publisher.none.fl_str_mv |
Soc General Microbiology |
publisher.none.fl_str_mv |
Soc General Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764145823580160 |