Avaliação da atividade biológica de metabólitos de Lippia alba (Verbenaceae), Plectranthus amboinicus e Plectranthus barbatus (Lamiaceae)

• Autor(a) principal: Santos, Nara Oshiro dos [UNIFESP]
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4102146; https://repositorio.unifesp.br/handle/11600/47196
• topic: Lippia alba; Plectranthus amboinicus; Plectranthus barbatus; Anti-leishmania activity; Antimicrobial activity; Antitumor activity; Essential oils; Lippia alba; Plectranthus amboinicus; Plectranthus barbatus; Atividade anti-leishmania; Atividade antimicrobiana; Atividade antitumoral; Óleos essenciais

This study aimed to identify substances with antimicrobial, antiparasitic and antitumor activities of extract and essential oil from the leaves of Lippia alba (Mill.) N.E. Brown and evaluate the antimicrobial and antitumor activities of essential oils from leaves of Plectranthus amboinicus (Lour.) Spreng and Plectranthus barbatus Andrews. The major compounds identified in the essential oil of L. alba leaves were monoterpenes nerol/geraniol (27%) and neral/geranial (22%), the ketone, 6-methyl-5- hepten-2-one (12%) and sesquiterpene, (E)-caryophyllene (9%). This oil showed cytotoxic activity in vitro against B16F10 (murine melanoma) with IC50 of 45.82 µg/mL. Furthermore, it was found that the oil has antimicrobial activity in vitro, inhibiting Gram-negative bacteria, except Pseudomonas aeruginosa, Gram-positive bacteria and yeasts. The dichloromethane phase obtained from the methanol extract of the leaves of this species showed activity against Leishmania (Leishmania) amazonensis with IC50 of 50.8 µg/mL and among the active groups of fractions were identified triterpenes mixture of oleanolic and ursolic acids, in addition to the steroid stigmasterol, all described in L. alba for the first time. The essential oil from leaves of P. amboinicus presented as major compounds carvacrol (38%), γ-terpinene (15%), (Z)-caryophyllene (14%) and p-cymene (12%), showing cytotoxic activity against B16F10 with IC50 similar to standard drug, however, twice more specific, in addition, showed antimicrobial activity against S. epidermidis, C. albicans, C. neoformans (serotype D), C. gattii (serotypes B and C) and S. cerevisiae. In turn, essential oil from leaves of P. barbatus presentes as major compounds (Z) -caryophyllene (18%), germacrene -D (17%), viridiflorene (14%), cyclosativene (10%) and α -pinene (9%), and has demonstrated activity against C. neoformans (serotype A) and C. gattii (serotype C).