Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33096 http://dx.doi.org/10.1208/s12248-010-9224-y |
Resumo: | Cells produce and use peptides in distinctive ways. in the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role. |
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Castro, Leandro Mantovani de [UNIFESP]Berti, Denise A.Russo, Lilian C.Coelho, VeronicaGozzo, Fabio C.Oliveira, Vitor [UNIFESP]Ferro, Emer Suavinho [UNIFESP]Inst Biomed SciUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Natl Inst Sci & TechnolUniversidade Estadual de Campinas (UNICAMP)2016-01-24T14:05:42Z2016-01-24T14:05:42Z2010-12-01Aaps Journal. New York: Springer, v. 12, n. 4, p. 608-616, 2010.1550-7416http://repositorio.unifesp.br/handle/11600/33096http://dx.doi.org/10.1208/s12248-010-9224-y10.1208/s12248-010-9224-yWOS:000288426100014Cells produce and use peptides in distinctive ways. in the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Financiadora de Estudos e Projetos (FINEP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto de Investigacao em Imunologia iii-Instituto Nacional de Ciencia e TecnologiaInst Biomed Sci, Dept Dev & Cell Biol, BR-05508000 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, BrazilUniv São Paulo, Immunol Lab, Heart Inst InCor, Sch Med, BR-05403001 São Paulo, BrazilNatl Inst Sci & Technol, INCT Inst Invest Immunol 3, BR-05403001 São Paulo, BrazilUniv Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP, BrazilUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, BrazilFAPESP: 04/04933-2FAPESP: 04/14846FAPESP: 2010/00828-0Financiadora de Estudos e Projetos (FINEP): A-03/134CNPq: 559698/2009-7Instituto de Investigacao em Imunologia iii-Instituto Nacional de Ciencia e Tecnologia: 573879/2008-7Web of Science608-616engSpringerAaps Journalhttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0info:eu-repo/semantics/openAccessintracellular peptidemass spectrometryMHC-I antigen presentationpeptidomeproteasomeSimilar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/330962023-02-15 11:39:38.46metadata only accessoai:repositorio.unifesp.br:11600/33096Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T14:39:38Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
title |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
spellingShingle |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis Castro, Leandro Mantovani de [UNIFESP] intracellular peptide mass spectrometry MHC-I antigen presentation peptidome proteasome |
title_short |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
title_full |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
title_fullStr |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
title_full_unstemmed |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
title_sort |
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis |
author |
Castro, Leandro Mantovani de [UNIFESP] |
author_facet |
Castro, Leandro Mantovani de [UNIFESP] Berti, Denise A. Russo, Lilian C. Coelho, Veronica Gozzo, Fabio C. Oliveira, Vitor [UNIFESP] Ferro, Emer Suavinho [UNIFESP] |
author_role |
author |
author2 |
Berti, Denise A. Russo, Lilian C. Coelho, Veronica Gozzo, Fabio C. Oliveira, Vitor [UNIFESP] Ferro, Emer Suavinho [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Inst Biomed Sci Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) Natl Inst Sci & Technol Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Castro, Leandro Mantovani de [UNIFESP] Berti, Denise A. Russo, Lilian C. Coelho, Veronica Gozzo, Fabio C. Oliveira, Vitor [UNIFESP] Ferro, Emer Suavinho [UNIFESP] |
dc.subject.eng.fl_str_mv |
intracellular peptide mass spectrometry MHC-I antigen presentation peptidome proteasome |
topic |
intracellular peptide mass spectrometry MHC-I antigen presentation peptidome proteasome |
description |
Cells produce and use peptides in distinctive ways. in the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-12-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:05:42Z |
dc.date.available.fl_str_mv |
2016-01-24T14:05:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Aaps Journal. New York: Springer, v. 12, n. 4, p. 608-616, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33096 http://dx.doi.org/10.1208/s12248-010-9224-y |
dc.identifier.issn.none.fl_str_mv |
1550-7416 |
dc.identifier.doi.none.fl_str_mv |
10.1208/s12248-010-9224-y |
dc.identifier.wos.none.fl_str_mv |
WOS:000288426100014 |
identifier_str_mv |
Aaps Journal. New York: Springer, v. 12, n. 4, p. 608-616, 2010. 1550-7416 10.1208/s12248-010-9224-y WOS:000288426100014 |
url |
http://repositorio.unifesp.br/handle/11600/33096 http://dx.doi.org/10.1208/s12248-010-9224-y |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Aaps Journal |
dc.rights.driver.fl_str_mv |
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
608-616 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1802764197857067008 |