Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/36675 https://dx.doi.org/10.1111/j.2047-2927.2013.00103.x |
Resumo: | Varicocoele is an important cause of male infertility. Normal male reproductive function and fertility depends on a delicate balance between androgen receptor (AR) and the classic oestrogen receptors ESR1 (ER alpha) and ESR2 (ER beta). Using a model of surgically induced varicocoele in rats, this study aimed to investigate the effects of varicocoele on the expression of AR, ESR1, ESR2 and G-protein coupled oestrogen receptor (GPER). Varicocoele did not affect the mRNA and protein expression of ESR1 and ESR2 in both testes. Varicocoele did not affect the mRNA and protein expression of GPER in the right testis, but slightly reduced the mRNA and increased the protein levels in the left testis. Varicocoele did not affect the mRNA for AR, but reduced the protein levels in both testes. A proteomic approach was used in an attempt to find differentially expressed targets with possible correlation with AR downregulation. Varicocoele caused the differential expression of 29 proteins. Six proteins were upregulated, including the receptor for activated C kinase 1 (RACK1), and 23 were downregulated, including dihydrolipoamide dehydrogenase, alpha-enolase and pyrophosphatase 1. Western blot analysis confirmed that varicocoele upregulated the expression of RACK1, a protein involved with tyrosine phosphorylation and regulation of AR transcriptional activity, AR metabolism and dynamics of the blood-testis barrier. in conclusion, this study suggests that varicocoele affects mechanisms that control AR expression and function. This regulation of AR may play an important role in the varicocoele-induced testicular dysfunction. Furthermore, varicocoele downregulates several other proteins in the testis that may be useful markers of spermatozoa function and male infertility. |
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Soares, Thiago da Silva [UNIFESP]Fernandes, Sheilla Alessandra Ferreira [UNIFESP]De Lima-Stein, Mariana Leão [UNIFESP]Stumpp, Taiza [UNIFESP]Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP]Lazari, Maria de Fatima Magalhaes [UNIFESP]Porto, Catarina Segreti [UNIFESP]Dept PharmacolDept Morphol & GenetUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:34:19Z2016-01-24T14:34:19Z2013-09-01Andrology. Hoboken: Wiley-Blackwell, v. 1, n. 5, p. 670-681, 2013.2047-2919https://repositorio.unifesp.br/handle/11600/36675https://dx.doi.org/10.1111/j.2047-2927.2013.00103.x10.1111/j.2047-2927.2013.00103.xWOS:000323385700003Varicocoele is an important cause of male infertility. Normal male reproductive function and fertility depends on a delicate balance between androgen receptor (AR) and the classic oestrogen receptors ESR1 (ER alpha) and ESR2 (ER beta). Using a model of surgically induced varicocoele in rats, this study aimed to investigate the effects of varicocoele on the expression of AR, ESR1, ESR2 and G-protein coupled oestrogen receptor (GPER). Varicocoele did not affect the mRNA and protein expression of ESR1 and ESR2 in both testes. Varicocoele did not affect the mRNA and protein expression of GPER in the right testis, but slightly reduced the mRNA and increased the protein levels in the left testis. Varicocoele did not affect the mRNA for AR, but reduced the protein levels in both testes. A proteomic approach was used in an attempt to find differentially expressed targets with possible correlation with AR downregulation. Varicocoele caused the differential expression of 29 proteins. Six proteins were upregulated, including the receptor for activated C kinase 1 (RACK1), and 23 were downregulated, including dihydrolipoamide dehydrogenase, alpha-enolase and pyrophosphatase 1. Western blot analysis confirmed that varicocoele upregulated the expression of RACK1, a protein involved with tyrosine phosphorylation and regulation of AR transcriptional activity, AR metabolism and dynamics of the blood-testis barrier. in conclusion, this study suggests that varicocoele affects mechanisms that control AR expression and function. This regulation of AR may play an important role in the varicocoele-induced testicular dysfunction. Furthermore, varicocoele downregulates several other proteins in the testis that may be useful markers of spermatozoa function and male infertility.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Dept Pharmacol, Sect Expt Endocrinol, São Paulo, BrazilDept Morphol & Genet, Dev Biol Lab, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, Escola Paulista Med, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, Escola Paulista Med, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, Escola Paulista Med, BR-04044020 São Paulo, BrazilFAPESP: 2007/52471-6FAPESP: 2010/52306-8Web of Science670-681engWiley-BlackwellAndrologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessAndrogen receptorOestrogen receptorsProteomicsReceptor for activated C kinase 1TestisVaricocoeleExperimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/366752023-10-06 17:29:53.681metadata only accessoai:repositorio.unifesp.br:11600/36675Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-10-06T20:29:53Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
title |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
spellingShingle |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor Soares, Thiago da Silva [UNIFESP] Androgen receptor Oestrogen receptors Proteomics Receptor for activated C kinase 1 Testis Varicocoele |
title_short |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
title_full |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
title_fullStr |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
title_full_unstemmed |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
title_sort |
Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor |
author |
Soares, Thiago da Silva [UNIFESP] |
author_facet |
Soares, Thiago da Silva [UNIFESP] Fernandes, Sheilla Alessandra Ferreira [UNIFESP] De Lima-Stein, Mariana Leão [UNIFESP] Stumpp, Taiza [UNIFESP] Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] Porto, Catarina Segreti [UNIFESP] |
author_role |
author |
author2 |
Fernandes, Sheilla Alessandra Ferreira [UNIFESP] De Lima-Stein, Mariana Leão [UNIFESP] Stumpp, Taiza [UNIFESP] Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] Porto, Catarina Segreti [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Dept Pharmacol Dept Morphol & Genet Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Soares, Thiago da Silva [UNIFESP] Fernandes, Sheilla Alessandra Ferreira [UNIFESP] De Lima-Stein, Mariana Leão [UNIFESP] Stumpp, Taiza [UNIFESP] Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] Porto, Catarina Segreti [UNIFESP] |
dc.subject.eng.fl_str_mv |
Androgen receptor Oestrogen receptors Proteomics Receptor for activated C kinase 1 Testis Varicocoele |
topic |
Androgen receptor Oestrogen receptors Proteomics Receptor for activated C kinase 1 Testis Varicocoele |
description |
Varicocoele is an important cause of male infertility. Normal male reproductive function and fertility depends on a delicate balance between androgen receptor (AR) and the classic oestrogen receptors ESR1 (ER alpha) and ESR2 (ER beta). Using a model of surgically induced varicocoele in rats, this study aimed to investigate the effects of varicocoele on the expression of AR, ESR1, ESR2 and G-protein coupled oestrogen receptor (GPER). Varicocoele did not affect the mRNA and protein expression of ESR1 and ESR2 in both testes. Varicocoele did not affect the mRNA and protein expression of GPER in the right testis, but slightly reduced the mRNA and increased the protein levels in the left testis. Varicocoele did not affect the mRNA for AR, but reduced the protein levels in both testes. A proteomic approach was used in an attempt to find differentially expressed targets with possible correlation with AR downregulation. Varicocoele caused the differential expression of 29 proteins. Six proteins were upregulated, including the receptor for activated C kinase 1 (RACK1), and 23 were downregulated, including dihydrolipoamide dehydrogenase, alpha-enolase and pyrophosphatase 1. Western blot analysis confirmed that varicocoele upregulated the expression of RACK1, a protein involved with tyrosine phosphorylation and regulation of AR transcriptional activity, AR metabolism and dynamics of the blood-testis barrier. in conclusion, this study suggests that varicocoele affects mechanisms that control AR expression and function. This regulation of AR may play an important role in the varicocoele-induced testicular dysfunction. Furthermore, varicocoele downregulates several other proteins in the testis that may be useful markers of spermatozoa function and male infertility. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-09-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:34:19Z |
dc.date.available.fl_str_mv |
2016-01-24T14:34:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Andrology. Hoboken: Wiley-Blackwell, v. 1, n. 5, p. 670-681, 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/36675 https://dx.doi.org/10.1111/j.2047-2927.2013.00103.x |
dc.identifier.issn.none.fl_str_mv |
2047-2919 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.2047-2927.2013.00103.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000323385700003 |
identifier_str_mv |
Andrology. Hoboken: Wiley-Blackwell, v. 1, n. 5, p. 670-681, 2013. 2047-2919 10.1111/j.2047-2927.2013.00103.x WOS:000323385700003 |
url |
https://repositorio.unifesp.br/handle/11600/36675 https://dx.doi.org/10.1111/j.2047-2927.2013.00103.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Andrology |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
670-681 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764184441585664 |