Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease

Detalhes bibliográficos
Autor(a) principal: Santos, V.n. dos [UNIFESP]
Data de Publicação: 2005
Outros Autores: Leite-Mór, Marilisa Moraes Barros [UNIFESP], Kondo, Mario [UNIFESP], Martins, J.r. [UNIFESP], Nader, Helena Bonciani [UNIFESP], Lanzoni, Valeria Pereira [UNIFESP], Parise, Edison Roberto [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2005000500012
http://repositorio.unifesp.br/handle/11600/2518
Resumo: Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients.
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spelling Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver diseaseHepatic fibrosisHyaluronanLamininNon-alcoholic fatty liver diseaseNon-alcoholic steatohepatitisType IV collagenHepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients.Universidade Federal de São Paulo (UNIFESP) Departamento de Medicina Seção de GastroenterologiaUniversidade Federal de São Paulo (UNIFESP) Departamento de Bioquímica Divisão de Biologia MolecularUniversidade Federal de São Paulo (UNIFESP) Departamento de PatologiaUNIFESP, Depto. de Medicina Seção de GastroenterologiaUNIFESP, Depto. de Bioquímica Divisão de Biologia MolecularUNIFESP, Depto. de PatologiaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Santos, V.n. dos [UNIFESP]Leite-Mór, Marilisa Moraes Barros [UNIFESP]Kondo, Mario [UNIFESP]Martins, J.r. [UNIFESP]Nader, Helena Bonciani [UNIFESP]Lanzoni, Valeria Pereira [UNIFESP]Parise, Edison Roberto [UNIFESP]2015-06-14T13:31:34Z2015-06-14T13:31:34Z2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion747-753application/pdfhttp://dx.doi.org/10.1590/S0100-879X2005000500012Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 5, p. 747-753, 2005.10.1590/S0100-879X2005000500012S0100-879X2005000500012.pdf0100-879XS0100-879X2005000500012http://repositorio.unifesp.br/handle/11600/2518WOS:000229605000012engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T23:11:35Zoai:repositorio.unifesp.br/:11600/2518Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T23:11:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
title Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
spellingShingle Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
Santos, V.n. dos [UNIFESP]
Hepatic fibrosis
Hyaluronan
Laminin
Non-alcoholic fatty liver disease
Non-alcoholic steatohepatitis
Type IV collagen
title_short Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
title_full Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
title_fullStr Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
title_full_unstemmed Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
title_sort Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
author Santos, V.n. dos [UNIFESP]
author_facet Santos, V.n. dos [UNIFESP]
Leite-Mór, Marilisa Moraes Barros [UNIFESP]
Kondo, Mario [UNIFESP]
Martins, J.r. [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Lanzoni, Valeria Pereira [UNIFESP]
Parise, Edison Roberto [UNIFESP]
author_role author
author2 Leite-Mór, Marilisa Moraes Barros [UNIFESP]
Kondo, Mario [UNIFESP]
Martins, J.r. [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Lanzoni, Valeria Pereira [UNIFESP]
Parise, Edison Roberto [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Santos, V.n. dos [UNIFESP]
Leite-Mór, Marilisa Moraes Barros [UNIFESP]
Kondo, Mario [UNIFESP]
Martins, J.r. [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Lanzoni, Valeria Pereira [UNIFESP]
Parise, Edison Roberto [UNIFESP]
dc.subject.por.fl_str_mv Hepatic fibrosis
Hyaluronan
Laminin
Non-alcoholic fatty liver disease
Non-alcoholic steatohepatitis
Type IV collagen
topic Hepatic fibrosis
Hyaluronan
Laminin
Non-alcoholic fatty liver disease
Non-alcoholic steatohepatitis
Type IV collagen
description Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-01
2015-06-14T13:31:34Z
2015-06-14T13:31:34Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2005000500012
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 5, p. 747-753, 2005.
10.1590/S0100-879X2005000500012
S0100-879X2005000500012.pdf
0100-879X
S0100-879X2005000500012
http://repositorio.unifesp.br/handle/11600/2518
WOS:000229605000012
url http://dx.doi.org/10.1590/S0100-879X2005000500012
http://repositorio.unifesp.br/handle/11600/2518
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 5, p. 747-753, 2005.
10.1590/S0100-879X2005000500012
S0100-879X2005000500012.pdf
0100-879X
S0100-879X2005000500012
WOS:000229605000012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 747-753
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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