Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2005000500012 http://repositorio.unifesp.br/handle/11600/2518 |
Resumo: | Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients. |
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Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver diseaseHepatic fibrosisHyaluronanLamininNon-alcoholic fatty liver diseaseNon-alcoholic steatohepatitisType IV collagenHepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients.Universidade Federal de São Paulo (UNIFESP) Departamento de Medicina Seção de GastroenterologiaUniversidade Federal de São Paulo (UNIFESP) Departamento de Bioquímica Divisão de Biologia MolecularUniversidade Federal de São Paulo (UNIFESP) Departamento de PatologiaUNIFESP, Depto. de Medicina Seção de GastroenterologiaUNIFESP, Depto. de Bioquímica Divisão de Biologia MolecularUNIFESP, Depto. de PatologiaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Santos, V.n. dos [UNIFESP]Leite-Mór, Marilisa Moraes Barros [UNIFESP]Kondo, Mario [UNIFESP]Martins, J.r. [UNIFESP]Nader, Helena Bonciani [UNIFESP]Lanzoni, Valeria Pereira [UNIFESP]Parise, Edison Roberto [UNIFESP]2015-06-14T13:31:34Z2015-06-14T13:31:34Z2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion747-753application/pdfhttp://dx.doi.org/10.1590/S0100-879X2005000500012Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 5, p. 747-753, 2005.10.1590/S0100-879X2005000500012S0100-879X2005000500012.pdf0100-879XS0100-879X2005000500012http://repositorio.unifesp.br/handle/11600/2518WOS:000229605000012engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T23:11:35Zoai:repositorio.unifesp.br/:11600/2518Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T23:11:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
title |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
spellingShingle |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease Santos, V.n. dos [UNIFESP] Hepatic fibrosis Hyaluronan Laminin Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis Type IV collagen |
title_short |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
title_full |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
title_fullStr |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
title_full_unstemmed |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
title_sort |
Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease |
author |
Santos, V.n. dos [UNIFESP] |
author_facet |
Santos, V.n. dos [UNIFESP] Leite-Mór, Marilisa Moraes Barros [UNIFESP] Kondo, Mario [UNIFESP] Martins, J.r. [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lanzoni, Valeria Pereira [UNIFESP] Parise, Edison Roberto [UNIFESP] |
author_role |
author |
author2 |
Leite-Mór, Marilisa Moraes Barros [UNIFESP] Kondo, Mario [UNIFESP] Martins, J.r. [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lanzoni, Valeria Pereira [UNIFESP] Parise, Edison Roberto [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Santos, V.n. dos [UNIFESP] Leite-Mór, Marilisa Moraes Barros [UNIFESP] Kondo, Mario [UNIFESP] Martins, J.r. [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lanzoni, Valeria Pereira [UNIFESP] Parise, Edison Roberto [UNIFESP] |
dc.subject.por.fl_str_mv |
Hepatic fibrosis Hyaluronan Laminin Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis Type IV collagen |
topic |
Hepatic fibrosis Hyaluronan Laminin Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis Type IV collagen |
description |
Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and aspartate aminotransferase values, which were due to the correlation between these parameters and the stage of fibrosis in the biopsy (Spearman's correlation coefficient, rS = 0.65, 0.62, 0.53, and 0.49, respectively). Analysis of the ROC curve showed that laminin values >282 ng/ml were those with the best diagnostic performance, with 87% accuracy. Association of laminin with type IV collagen showed improvement in the positive predictive value (100%), but with reduction in diagnostic sensitivity (64%). When compared with the criteria of Ratziu et al. [Gastroenterology (2000) 118: 1117-1123] for the diagnosis of septal fibrosis, laminin values presented a better diagnostic accuracy (83 vs 70%). Determination of extracellular matrix components in serum, especially of laminin, may identify patients with non-alcoholic fatty liver disease and fibrosis and these components may be used as indicators for liver biopsy in these patients. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-05-01 2015-06-14T13:31:34Z 2015-06-14T13:31:34Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2005000500012 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 5, p. 747-753, 2005. 10.1590/S0100-879X2005000500012 S0100-879X2005000500012.pdf 0100-879X S0100-879X2005000500012 http://repositorio.unifesp.br/handle/11600/2518 WOS:000229605000012 |
url |
http://dx.doi.org/10.1590/S0100-879X2005000500012 http://repositorio.unifesp.br/handle/11600/2518 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 5, p. 747-753, 2005. 10.1590/S0100-879X2005000500012 S0100-879X2005000500012.pdf 0100-879X S0100-879X2005000500012 WOS:000229605000012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
747-753 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268277993504768 |