Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis

Detalhes bibliográficos
Autor(a) principal: Toledo, Mayte dos Santos [UNIFESP]
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000003smr
Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6614954
https://repositorio.unifesp.br/handle/11600/52753
Resumo: Leishmaniasis is a group of diseases caused by protozoa belonging to the genus Leishmania. The immune response to leishmaniasis is complex and the outcome of the infection depends on several factors, such as genetic composition of Leishmania species and host immunity. Macrophages are known to play a central role in infection by Leishmania parasites and their activation may be influenced by several cell types, such as Th1, Th2, and B-1 lymphocytes. B-1 cells are a subtype of B lymphocytes with peculiar functions in immunity. These cells are able to produce regulatory cytokines (mainly IL-10), natural antibodies and differentiate into phagocytic cells. In addition to cytokines and antibodies, B cells can release different types of extracellular vesicles (EVs) with an important role in intercellular communication, with role in regulating activation and/or modulation of the immune system. However, the production of EVs by B-1 cells and their role in immunity have not yet been demonstrated The aims of this work were to characterize the EVs released by B-1 cells; to study the influence of these EVs on the modulation of lineage macrophages and medullary macrophages from different mouse strains; and to evaluate the role of EVs from B-1 cells in the course of experimental infection by L. (L.) amazonensis. Our results showed that EVs released by B-1 cells infected with the parasite led to a differential activation of J774A.1 macrophages, macrophages from BALB/c and C57BL/6 mice. In addition, EVs released spontaneously by B-1 cells led to a reduction in the lesion progression and parasite burden in BALB/c mice experimentally infected. Thus, this study demonstrated that B-1 cells can release EVs that are capable of altering the functions of macrophages in vitro. In vivo these EVs altered the course of L. (L.) amazonensis infection. This is the first evidence that EVs from B-1 cells can act as a new mechanism of cellular communication between macrophages and B-1 cells.
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spelling Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) AmazonensisB-1 CellsMacrophageLeishmania (L) amazonensisCytokinesExtracellular vesiclesCélulas B-1MacrófagosLeishmania (L) amazonensisCitocinasVesículas extracelularesLeishmaniasis is a group of diseases caused by protozoa belonging to the genus Leishmania. The immune response to leishmaniasis is complex and the outcome of the infection depends on several factors, such as genetic composition of Leishmania species and host immunity. Macrophages are known to play a central role in infection by Leishmania parasites and their activation may be influenced by several cell types, such as Th1, Th2, and B-1 lymphocytes. B-1 cells are a subtype of B lymphocytes with peculiar functions in immunity. These cells are able to produce regulatory cytokines (mainly IL-10), natural antibodies and differentiate into phagocytic cells. In addition to cytokines and antibodies, B cells can release different types of extracellular vesicles (EVs) with an important role in intercellular communication, with role in regulating activation and/or modulation of the immune system. However, the production of EVs by B-1 cells and their role in immunity have not yet been demonstrated The aims of this work were to characterize the EVs released by B-1 cells; to study the influence of these EVs on the modulation of lineage macrophages and medullary macrophages from different mouse strains; and to evaluate the role of EVs from B-1 cells in the course of experimental infection by L. (L.) amazonensis. Our results showed that EVs released by B-1 cells infected with the parasite led to a differential activation of J774A.1 macrophages, macrophages from BALB/c and C57BL/6 mice. In addition, EVs released spontaneously by B-1 cells led to a reduction in the lesion progression and parasite burden in BALB/c mice experimentally infected. Thus, this study demonstrated that B-1 cells can release EVs that are capable of altering the functions of macrophages in vitro. In vivo these EVs altered the course of L. (L.) amazonensis infection. This is the first evidence that EVs from B-1 cells can act as a new mechanism of cellular communication between macrophages and B-1 cells.As leishmanioses constituem um grupo de doenças causadas por protozoários pertencentes ao gênero Leishmania. A resposta imune para a leishmaniose é complexa e o resultado da infecção depende de vários fatores, como composição genética das várias espécies de Leishmania e da imunidade do hospedeiro. Sabe-se que macrófagos têm papel central na infecção por parasitas do gênero Leishmania e sua ativação pode ser influenciada por diversos tipos celulares, como células Th1, Th2, e linfócitos B-1. Células B-1 são um subtipo de linfócitos B com funções peculiares na imunidade. Essas células são capazes de produzir citocinas reguladoras (principalmente IL-10), anticorpos naturais e se diferenciar em células fagocíticas. Além de citocinas e anticorpos, células B podem liberar diferentes tipos de vesículas extracelulares (EVs) com papel importante na comunicação intercelular, podendo levar a ativação e/ou regulação do sistema imunológico. Contudo, ainda não foi demonstrado a produção de EVs por células B-1 e seu papel na imunidade. Os objetivos deste trabalho foram caracterizar as EVs liberadas por células B-1; estudar a influência dessas EVs na modulação de macrófagos de linhagem e macrófagos medulares de distintas linhagens de camundongos; e avaliar o papel de EVs de células B-1 no curso da infeção experimental por L. (L.) amazonensis. Nossos resultados mostraram que EVs liberadas por células B-1 infectadas com o parasita modularam diferentemente a ativação de macrófagos da linhagem J774A.1, macrófagos medulares provenientes de camundongos BALB/c e C57BL/6. EVs liberadas espontaneamente por células B-1 levaram a redução na progressão da lesão e da carga parasitária em camundongos BALB/c. Assim, este estudo demonstrou que células B-1 podem liberar EVs que influenciam a modulação de macrófagos in vitro além de alterar o curso da infecção experimental por L. (L.) amazonensis. Esta é a primeira evidência da liberação de EVs por células B-1 e o seu papel na comunicação macrófagos-células B-1.Dados abertos - Sucupira - Teses e dissertações (2018)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São PauloXander, Patricia [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Toledo, Mayte dos Santos [UNIFESP]2020-03-25T12:10:29Z2020-03-25T12:10:29Z2018-03-26info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion103 p.application/pdfhttps://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=66149542018-0696.pdfhttps://repositorio.unifesp.br/handle/11600/52753ark:/48912/0013000003smrporinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-02T18:13:36Zoai:repositorio.unifesp.br/:11600/52753Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T19:55:25.106719Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
title Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
spellingShingle Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
Toledo, Mayte dos Santos [UNIFESP]
B-1 Cells
Macrophage
Leishmania (L) amazonensis
Cytokines
Extracellular vesicles
Células B-1
Macrófagos
Leishmania (L) amazonensis
Citocinas
Vesículas extracelulares
title_short Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
title_full Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
title_fullStr Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
title_full_unstemmed Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
title_sort Caracterização morfológica e funcional de vesículas extracelulares liberadas por células B-1 infectadas por Leishmania (Leishmania) Amazonensis
author Toledo, Mayte dos Santos [UNIFESP]
author_facet Toledo, Mayte dos Santos [UNIFESP]
author_role author
dc.contributor.none.fl_str_mv Xander, Patricia [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Toledo, Mayte dos Santos [UNIFESP]
dc.subject.por.fl_str_mv B-1 Cells
Macrophage
Leishmania (L) amazonensis
Cytokines
Extracellular vesicles
Células B-1
Macrófagos
Leishmania (L) amazonensis
Citocinas
Vesículas extracelulares
topic B-1 Cells
Macrophage
Leishmania (L) amazonensis
Cytokines
Extracellular vesicles
Células B-1
Macrófagos
Leishmania (L) amazonensis
Citocinas
Vesículas extracelulares
description Leishmaniasis is a group of diseases caused by protozoa belonging to the genus Leishmania. The immune response to leishmaniasis is complex and the outcome of the infection depends on several factors, such as genetic composition of Leishmania species and host immunity. Macrophages are known to play a central role in infection by Leishmania parasites and their activation may be influenced by several cell types, such as Th1, Th2, and B-1 lymphocytes. B-1 cells are a subtype of B lymphocytes with peculiar functions in immunity. These cells are able to produce regulatory cytokines (mainly IL-10), natural antibodies and differentiate into phagocytic cells. In addition to cytokines and antibodies, B cells can release different types of extracellular vesicles (EVs) with an important role in intercellular communication, with role in regulating activation and/or modulation of the immune system. However, the production of EVs by B-1 cells and their role in immunity have not yet been demonstrated The aims of this work were to characterize the EVs released by B-1 cells; to study the influence of these EVs on the modulation of lineage macrophages and medullary macrophages from different mouse strains; and to evaluate the role of EVs from B-1 cells in the course of experimental infection by L. (L.) amazonensis. Our results showed that EVs released by B-1 cells infected with the parasite led to a differential activation of J774A.1 macrophages, macrophages from BALB/c and C57BL/6 mice. In addition, EVs released spontaneously by B-1 cells led to a reduction in the lesion progression and parasite burden in BALB/c mice experimentally infected. Thus, this study demonstrated that B-1 cells can release EVs that are capable of altering the functions of macrophages in vitro. In vivo these EVs altered the course of L. (L.) amazonensis infection. This is the first evidence that EVs from B-1 cells can act as a new mechanism of cellular communication between macrophages and B-1 cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-26
2020-03-25T12:10:29Z
2020-03-25T12:10:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6614954
2018-0696.pdf
https://repositorio.unifesp.br/handle/11600/52753
dc.identifier.dark.fl_str_mv ark:/48912/0013000003smr
url https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6614954
https://repositorio.unifesp.br/handle/11600/52753
identifier_str_mv 2018-0696.pdf
ark:/48912/0013000003smr
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 103 p.
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de São Paulo
publisher.none.fl_str_mv Universidade Federal de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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