Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38334 http://dx.doi.org/10.1371/journal.pone.0110747 |
Resumo: | Introduction: Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia.Objective: To characterize the lipid profile in the placenta and plasma of patients with preeclampsia.Methodology: Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry.Results: Approximately 200 lipid signals were quantified. the most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p, 0.0001) and PK04 (p<0.0001).Conclusion: Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia. |
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Korkes, Henri Augusto [UNIFESP]Sass, Nelson [UNIFESP]Moron, Antonio F. [UNIFESP]Câmara, Niels Olsen Saraiva [UNIFESP]Bonetti, Tatiana [UNIFESP]Cerdeira, Ana SofiaCotrim Guerreiro Da Silva, Ismael Dale [UNIFESP]De Oliveira, Leandro [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Sch Matern Vila Nova CachoeirinhaUniversidade de São Paulo (USP)Harvard Univ2016-01-24T14:38:01Z2016-01-24T14:38:01Z2014-10-17Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/38334http://dx.doi.org/10.1371/journal.pone.0110747WOS000345204100106.pdf10.1371/journal.pone.0110747WOS:000345204100106Introduction: Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia.Objective: To characterize the lipid profile in the placenta and plasma of patients with preeclampsia.Methodology: Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry.Results: Approximately 200 lipid signals were quantified. the most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p, 0.0001) and PK04 (p<0.0001).Conclusion: Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Foundation for Research Support of the State of São PauloUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilSch Matern Vila Nova Cachoeirinha, Lab Clin & Expt Invest, São Paulo, BrazilUniv São Paulo, Dept Immunol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, BrazilHarvard Univ, Beth Israel Deaconess Med Ctr, Dept Med, Sch Med, Boston, MA 02215 USAUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, BrazilCNPq: 476486/2011-4Foundation for Research Support of the State of São Paulo: 12/02270-2Web of Science8engPublic Library SciencePlos OneLipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000345204100106.pdfapplication/pdf372677${dspace.ui.url}/bitstream/11600/38334/1/WOS000345204100106.pdfb06969dff94b5006ae94e6576deabdf3MD51open accessTEXTWOS000345204100106.pdf.txtWOS000345204100106.pdf.txtExtracted texttext/plain45027${dspace.ui.url}/bitstream/11600/38334/2/WOS000345204100106.pdf.txt975db3eae7ea20d9df7c1814324396cfMD52open access11600/383342022-06-02 09:05:59.513open accessoai:repositorio.unifesp.br:11600/38334Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:05:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
title |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
spellingShingle |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia Korkes, Henri Augusto [UNIFESP] |
title_short |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
title_full |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
title_fullStr |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
title_full_unstemmed |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
title_sort |
Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia |
author |
Korkes, Henri Augusto [UNIFESP] |
author_facet |
Korkes, Henri Augusto [UNIFESP] Sass, Nelson [UNIFESP] Moron, Antonio F. [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] Bonetti, Tatiana [UNIFESP] Cerdeira, Ana Sofia Cotrim Guerreiro Da Silva, Ismael Dale [UNIFESP] De Oliveira, Leandro [UNIFESP] |
author_role |
author |
author2 |
Sass, Nelson [UNIFESP] Moron, Antonio F. [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] Bonetti, Tatiana [UNIFESP] Cerdeira, Ana Sofia Cotrim Guerreiro Da Silva, Ismael Dale [UNIFESP] De Oliveira, Leandro [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Sch Matern Vila Nova Cachoeirinha Universidade de São Paulo (USP) Harvard Univ |
dc.contributor.author.fl_str_mv |
Korkes, Henri Augusto [UNIFESP] Sass, Nelson [UNIFESP] Moron, Antonio F. [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] Bonetti, Tatiana [UNIFESP] Cerdeira, Ana Sofia Cotrim Guerreiro Da Silva, Ismael Dale [UNIFESP] De Oliveira, Leandro [UNIFESP] |
description |
Introduction: Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia.Objective: To characterize the lipid profile in the placenta and plasma of patients with preeclampsia.Methodology: Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry.Results: Approximately 200 lipid signals were quantified. the most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p, 0.0001) and PK04 (p<0.0001).Conclusion: Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-10-17 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:01Z |
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2016-01-24T14:38:01Z |
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Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014. |
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http://repositorio.unifesp.br/handle/11600/38334 http://dx.doi.org/10.1371/journal.pone.0110747 |
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1932-6203 |
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WOS000345204100106.pdf |
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10.1371/journal.pone.0110747 |
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Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014. 1932-6203 WOS000345204100106.pdf 10.1371/journal.pone.0110747 WOS:000345204100106 |
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http://repositorio.unifesp.br/handle/11600/38334 http://dx.doi.org/10.1371/journal.pone.0110747 |
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