Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model

Detalhes bibliográficos
Autor(a) principal: Armstrong, Dinani Matoso Fialho de Oliveira
Data de Publicação: 2013
Outros Autores: Armstrong, Anderson da Costa, Figueiredo, Regina Célia Bressan Queiroz, Florentino, Joao Eduardo, Saad, Paulo Fernandes [UNIFESP], Fox-Talbot, Karen, Halushka, Marc Kenneth, Berkowitz, Dan E., Taha, Murched Omar [UNIFESP], Fagundes, Djalma José [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/7683
http://dx.doi.org/10.1590/S0102-86502013000400008
Resumo: PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.
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spelling Armstrong, Dinani Matoso Fialho de OliveiraArmstrong, Anderson da CostaFigueiredo, Regina Célia Bressan QueirozFlorentino, Joao EduardoSaad, Paulo Fernandes [UNIFESP]Fox-Talbot, KarenHalushka, Marc KennethBerkowitz, Dan E.Taha, Murched Omar [UNIFESP]Fagundes, Djalma José [UNIFESP]Sao Francisco Valley Federal University Department of SurgeryUNIVASF Department of CardiologyResearch Center Aggeu Magalhaes Department of MicrobiologyPernambuco Federal UniversityUNIVASF Department of SurgeryJohns Hopkins University Department of PathologyJohns Hopkins University Department of Anesthesiology and Critical Care MedicineUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:45:21Z2015-06-14T13:45:21Z2013-04-01Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 4, p. 282-287, 2013.0102-8650http://repositorio.unifesp.br/handle/11600/7683http://dx.doi.org/10.1590/S0102-86502013000400008S0102-86502013000400008.pdfS0102-8650201300040000810.1590/S0102-86502013000400008WOS:000317522300008PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.Sao Francisco Valley Federal University Department of SurgeryUNIVASF Department of CardiologyResearch Center Aggeu Magalhaes Department of MicrobiologyPernambuco Federal UniversityUNIVASF Department of SurgeryJohns Hopkins University Department of PathologyJohns Hopkins University Department of Anesthesiology and Critical Care MedicineUniversidade Federal de São Paulo (UNIFESP) Department of Surgery Operative Technique and Experimental Surgery DivisionUNIFESP, Department of Surgery Operative Technique and Experimental Surgery DivisionSciELO282-287engSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirurgica BrasileiraReperfusion InjuryMuscle, SkeletalPhosphodiesteraseInhibitorsCaspase 3RatsSildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0102-86502013000400008.pdfapplication/pdf545241${dspace.ui.url}/bitstream/11600/7683/1/S0102-86502013000400008.pdf47a56c1f69e6974d716b983b33b536eeMD51open accessTEXTS0102-86502013000400008.pdf.txtS0102-86502013000400008.pdf.txtExtracted texttext/plain22896${dspace.ui.url}/bitstream/11600/7683/2/S0102-86502013000400008.pdf.txtbf4a45980fd9f05d758d71fd3414942cMD52open access11600/76832021-09-30 10:59:30.927open accessoai:repositorio.unifesp.br:11600/7683Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-30T13:59:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
title Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
spellingShingle Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
Armstrong, Dinani Matoso Fialho de Oliveira
Reperfusion Injury
Muscle, Skeletal
Phosphodiesterase
Inhibitors
Caspase 3
Rats
title_short Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
title_full Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
title_fullStr Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
title_full_unstemmed Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
title_sort Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model
author Armstrong, Dinani Matoso Fialho de Oliveira
author_facet Armstrong, Dinani Matoso Fialho de Oliveira
Armstrong, Anderson da Costa
Figueiredo, Regina Célia Bressan Queiroz
Florentino, Joao Eduardo
Saad, Paulo Fernandes [UNIFESP]
Fox-Talbot, Karen
Halushka, Marc Kenneth
Berkowitz, Dan E.
Taha, Murched Omar [UNIFESP]
Fagundes, Djalma José [UNIFESP]
author_role author
author2 Armstrong, Anderson da Costa
Figueiredo, Regina Célia Bressan Queiroz
Florentino, Joao Eduardo
Saad, Paulo Fernandes [UNIFESP]
Fox-Talbot, Karen
Halushka, Marc Kenneth
Berkowitz, Dan E.
Taha, Murched Omar [UNIFESP]
Fagundes, Djalma José [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Sao Francisco Valley Federal University Department of Surgery
UNIVASF Department of Cardiology
Research Center Aggeu Magalhaes Department of Microbiology
Pernambuco Federal University
UNIVASF Department of Surgery
Johns Hopkins University Department of Pathology
Johns Hopkins University Department of Anesthesiology and Critical Care Medicine
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Armstrong, Dinani Matoso Fialho de Oliveira
Armstrong, Anderson da Costa
Figueiredo, Regina Célia Bressan Queiroz
Florentino, Joao Eduardo
Saad, Paulo Fernandes [UNIFESP]
Fox-Talbot, Karen
Halushka, Marc Kenneth
Berkowitz, Dan E.
Taha, Murched Omar [UNIFESP]
Fagundes, Djalma José [UNIFESP]
dc.subject.eng.fl_str_mv Reperfusion Injury
Muscle, Skeletal
Phosphodiesterase
Inhibitors
Caspase 3
Rats
topic Reperfusion Injury
Muscle, Skeletal
Phosphodiesterase
Inhibitors
Caspase 3
Rats
description PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.
publishDate 2013
dc.date.issued.fl_str_mv 2013-04-01
dc.date.accessioned.fl_str_mv 2015-06-14T13:45:21Z
dc.date.available.fl_str_mv 2015-06-14T13:45:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 4, p. 282-287, 2013.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/7683
http://dx.doi.org/10.1590/S0102-86502013000400008
dc.identifier.issn.none.fl_str_mv 0102-8650
dc.identifier.file.none.fl_str_mv S0102-86502013000400008.pdf
dc.identifier.scielo.none.fl_str_mv S0102-86502013000400008
dc.identifier.doi.none.fl_str_mv 10.1590/S0102-86502013000400008
dc.identifier.wos.none.fl_str_mv WOS:000317522300008
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 4, p. 282-287, 2013.
0102-8650
S0102-86502013000400008.pdf
S0102-86502013000400008
10.1590/S0102-86502013000400008
WOS:000317522300008
url http://repositorio.unifesp.br/handle/11600/7683
http://dx.doi.org/10.1590/S0102-86502013000400008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 282-287
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
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