Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/29208 http://dx.doi.org/10.1016/j.virol.2006.04.039 |
Resumo: | We analyzed gp120V3 HIV-1 env region genetic diversity of 27 patients failing antiretrovirals and subjected to 12-week structured treatment interruption (STI). Based on heteroduplex mobility assays, eight patients presented low pre- and post-STI genetic diversity (G1); five presented high pre-STI but low post-STI diversity (G2); five presented low pre-STI and high post-STI diversity (G3); and nine, high pre- and post-STI diversity (G4). One patient from G1, two from G2 and two from G3 were subjected to proviral DNA end-point PCR and sequencing. in three patients, the dramatic disturbance caused by STI resulted in ancestral viral progeny activation, which repopulated the cell reservoir. in two patients presenting highly homogeneous sequences and low immune selective pressure (dN/dS ratio < 1), this phenomenon was not observed. the mechanisms involved in viral evolution, in which antiretroviral therapy also applies selective pressure, sometimes affects coreceptor usage of circulating viruses, leading to the suppression of x4 strains. (c) 2006 Published by Elsevier Inc. |
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Silva, Wilson Pereira [UNIFESP]Santos, Domingos Ezenildo Matos dos [UNIFESP]Leal, Elcio de Souza [UNIFESP]Brunstein, Adriana [UNIFESP]Sucupira, Maria Cecília Araripe [UNIFESP]Sabino, Ester Cerdeira [UNIFESP]Diaz, Ricardo Sobhie [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Fundacao Pro Sangue2016-01-24T12:41:32Z2016-01-24T12:41:32Z2006-10-10Virology. San Diego: Academic Press Inc Elsevier Science, v. 354, n. 1, p. 35-47, 2006.0042-6822http://repositorio.unifesp.br/handle/11600/29208http://dx.doi.org/10.1016/j.virol.2006.04.039WOS000241178500005.pdf10.1016/j.virol.2006.04.039WOS:000241178500005We analyzed gp120V3 HIV-1 env region genetic diversity of 27 patients failing antiretrovirals and subjected to 12-week structured treatment interruption (STI). Based on heteroduplex mobility assays, eight patients presented low pre- and post-STI genetic diversity (G1); five presented high pre-STI but low post-STI diversity (G2); five presented low pre-STI and high post-STI diversity (G3); and nine, high pre- and post-STI diversity (G4). One patient from G1, two from G2 and two from G3 were subjected to proviral DNA end-point PCR and sequencing. in three patients, the dramatic disturbance caused by STI resulted in ancestral viral progeny activation, which repopulated the cell reservoir. in two patients presenting highly homogeneous sequences and low immune selective pressure (dN/dS ratio < 1), this phenomenon was not observed. the mechanisms involved in viral evolution, in which antiretroviral therapy also applies selective pressure, sometimes affects coreceptor usage of circulating viruses, leading to the suppression of x4 strains. (c) 2006 Published by Elsevier Inc.Universidade Federal de São Paulo, Escola Paulista Med, Lab Retrovirol, BR-04039032 São Paulo, BrazilFundacao Pro Sangue, BR-05403000 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Lab Retrovirol, BR-04039032 São Paulo, BrazilWeb of Science35-47engElsevier B.V.Virologyhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessstructured treatment interruptionHIV-1envelope geneantiretroviral resistanceReactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruptioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000241178500005.pdfapplication/pdf1516511${dspace.ui.url}/bitstream/11600/29208/1/WOS000241178500005.pdf42f9b84488a78e45a7fcf22a083ee0f1MD51open accessTEXTWOS000241178500005.pdf.txtWOS000241178500005.pdf.txtExtracted texttext/plain49200${dspace.ui.url}/bitstream/11600/29208/2/WOS000241178500005.pdf.txt5a1a9d5cf50d6556e6394416fbc12bf0MD52open access11600/292082023-02-15 09:10:17.898open accessoai:repositorio.unifesp.br:11600/29208Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T12:10:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
title |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
spellingShingle |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption Silva, Wilson Pereira [UNIFESP] structured treatment interruption HIV-1 envelope gene antiretroviral resistance |
title_short |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
title_full |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
title_fullStr |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
title_full_unstemmed |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
title_sort |
Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption |
author |
Silva, Wilson Pereira [UNIFESP] |
author_facet |
Silva, Wilson Pereira [UNIFESP] Santos, Domingos Ezenildo Matos dos [UNIFESP] Leal, Elcio de Souza [UNIFESP] Brunstein, Adriana [UNIFESP] Sucupira, Maria Cecília Araripe [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] |
author_role |
author |
author2 |
Santos, Domingos Ezenildo Matos dos [UNIFESP] Leal, Elcio de Souza [UNIFESP] Brunstein, Adriana [UNIFESP] Sucupira, Maria Cecília Araripe [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fundacao Pro Sangue |
dc.contributor.author.fl_str_mv |
Silva, Wilson Pereira [UNIFESP] Santos, Domingos Ezenildo Matos dos [UNIFESP] Leal, Elcio de Souza [UNIFESP] Brunstein, Adriana [UNIFESP] Sucupira, Maria Cecília Araripe [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] |
dc.subject.eng.fl_str_mv |
structured treatment interruption HIV-1 envelope gene antiretroviral resistance |
topic |
structured treatment interruption HIV-1 envelope gene antiretroviral resistance |
description |
We analyzed gp120V3 HIV-1 env region genetic diversity of 27 patients failing antiretrovirals and subjected to 12-week structured treatment interruption (STI). Based on heteroduplex mobility assays, eight patients presented low pre- and post-STI genetic diversity (G1); five presented high pre-STI but low post-STI diversity (G2); five presented low pre-STI and high post-STI diversity (G3); and nine, high pre- and post-STI diversity (G4). One patient from G1, two from G2 and two from G3 were subjected to proviral DNA end-point PCR and sequencing. in three patients, the dramatic disturbance caused by STI resulted in ancestral viral progeny activation, which repopulated the cell reservoir. in two patients presenting highly homogeneous sequences and low immune selective pressure (dN/dS ratio < 1), this phenomenon was not observed. the mechanisms involved in viral evolution, in which antiretroviral therapy also applies selective pressure, sometimes affects coreceptor usage of circulating viruses, leading to the suppression of x4 strains. (c) 2006 Published by Elsevier Inc. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-10-10 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:41:32Z |
dc.date.available.fl_str_mv |
2016-01-24T12:41:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Virology. San Diego: Academic Press Inc Elsevier Science, v. 354, n. 1, p. 35-47, 2006. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/29208 http://dx.doi.org/10.1016/j.virol.2006.04.039 |
dc.identifier.issn.none.fl_str_mv |
0042-6822 |
dc.identifier.file.none.fl_str_mv |
WOS000241178500005.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.virol.2006.04.039 |
dc.identifier.wos.none.fl_str_mv |
WOS:000241178500005 |
identifier_str_mv |
Virology. San Diego: Academic Press Inc Elsevier Science, v. 354, n. 1, p. 35-47, 2006. 0042-6822 WOS000241178500005.pdf 10.1016/j.virol.2006.04.039 WOS:000241178500005 |
url |
http://repositorio.unifesp.br/handle/11600/29208 http://dx.doi.org/10.1016/j.virol.2006.04.039 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Virology |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
35-47 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
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