Malnutrition and experimental lung allergy
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/43281 http://dx.doi.org/10.1111/j.1365-2222.1997.tb01159.x |
Resumo: | Background Although it is commonly accepted that the immune response is affected by malnutrition there are very few data about its effect in allergic diseases.Objective The aim of the present study was to investigate the effect of malnutrition in allergic lung inflammation.Methods An anaphylactic reaction was induced in rat lungs and the increased vascular permeability was measured in the trachea, internal and external bronchi and parenchyma by the Evans blue extravasation method. These studies were conducted in two dietary groups: one fed a normoproteic diet (18%) and the other a hypoproteic diet (4.5%). When the animals were 60 days old the group fed the hypoproteic diet presented a reduction of 77.86% in bodyweight, 63.3% in food intake and 36% in plasma protein concentration characterizing a severe protein-calorie malnutrition.Results The anaphylactic reaction in the lungs induced a significant increase in vascular permeability in the trachea and bronchi of both dietary groups. However, the intensity of this effect was significantly lower in the malnourished group. Analysis of immunoglobulin isotypes in the serum by ELISA showed that whereas IgG1 and IgG2a levels were similar in both groups, the levels of IgE were significantly lower in the malnourished animals. Moreover, the levels of antigen-specific IgG1, IgG2a and IgE were all significantly inhibited by the protein-calorie malnutrition. When antibodies were passively transfered to the malnourished rats, they developed a reaction as intense as the normoproteic group.Conclusion These results suggest that the capacity to release inflammatory mediators and the vascular response to these mediators is not affected by this type of malnutrition and, therefore, the diminished response of the airways reported here is probably due to the lower levels of anaphylactic antibodies produced by the malnourished rats. |
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Cunha, M. G.Naspitz, Charles Kirov [UNIFESP]MacedoSoares, F.Amancio, Olga Maria Silverio [UNIFESP]Jancar, Sonia [UNIFESP]Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)INST BUTANTAN2018-06-15T16:39:18Z2018-06-15T16:39:18Z1997-10-01Clinical And Experimental Allergy. Oxford: Blackwell Science Ltd, v. 27, n. 10, p. 1212-1218, 1997.0954-7894http://repositorio.unifesp.br/11600/43281http://dx.doi.org/10.1111/j.1365-2222.1997.tb01159.x10.1111/j.1365-2222.1997.tb01159.xWOS:A1997YB77500014Background Although it is commonly accepted that the immune response is affected by malnutrition there are very few data about its effect in allergic diseases.Objective The aim of the present study was to investigate the effect of malnutrition in allergic lung inflammation.Methods An anaphylactic reaction was induced in rat lungs and the increased vascular permeability was measured in the trachea, internal and external bronchi and parenchyma by the Evans blue extravasation method. These studies were conducted in two dietary groups: one fed a normoproteic diet (18%) and the other a hypoproteic diet (4.5%). When the animals were 60 days old the group fed the hypoproteic diet presented a reduction of 77.86% in bodyweight, 63.3% in food intake and 36% in plasma protein concentration characterizing a severe protein-calorie malnutrition.Results The anaphylactic reaction in the lungs induced a significant increase in vascular permeability in the trachea and bronchi of both dietary groups. However, the intensity of this effect was significantly lower in the malnourished group. Analysis of immunoglobulin isotypes in the serum by ELISA showed that whereas IgG1 and IgG2a levels were similar in both groups, the levels of IgE were significantly lower in the malnourished animals. Moreover, the levels of antigen-specific IgG1, IgG2a and IgE were all significantly inhibited by the protein-calorie malnutrition. When antibodies were passively transfered to the malnourished rats, they developed a reaction as intense as the normoproteic group.Conclusion These results suggest that the capacity to release inflammatory mediators and the vascular response to these mediators is not affected by this type of malnutrition and, therefore, the diminished response of the airways reported here is probably due to the lower levels of anaphylactic antibodies produced by the malnourished rats.UNIV SAO PAULO,INST BIOMED SCI,DEPT IMMUNOL,BR-05508900 SAO PAULO,BRAZILUNIV FED SAO PAULO,DEPT PAEDIAT,DIV CLIN IMMUNOL & ALLERGY,SAO PAULO,BRAZILINST BUTANTAN,IMMUNOPATHOL LAB,SAO PAULO,BRAZILUNIV FED SAO PAULO,DEPT PAEDIAT,DIV CLIN IMMUNOL & ALLERGY,SAO PAULO,BRAZILWeb of Science1212-1218engBlackwell Science LtdClinical And Experimental Allergymalnutritionlung allergyvascular permeabilityrat airwaysprotein-calorie deficiencyMalnutrition and experimental lung allergyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/432812022-02-07 20:58:45.99metadata only accessoai:repositorio.unifesp.br:11600/43281Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:09:40.109841Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Malnutrition and experimental lung allergy |
title |
Malnutrition and experimental lung allergy |
spellingShingle |
Malnutrition and experimental lung allergy Cunha, M. G. malnutrition lung allergy vascular permeability rat airways protein-calorie deficiency |
title_short |
Malnutrition and experimental lung allergy |
title_full |
Malnutrition and experimental lung allergy |
title_fullStr |
Malnutrition and experimental lung allergy |
title_full_unstemmed |
Malnutrition and experimental lung allergy |
title_sort |
Malnutrition and experimental lung allergy |
author |
Cunha, M. G. |
author_facet |
Cunha, M. G. Naspitz, Charles Kirov [UNIFESP] MacedoSoares, F. Amancio, Olga Maria Silverio [UNIFESP] Jancar, Sonia [UNIFESP] |
author_role |
author |
author2 |
Naspitz, Charles Kirov [UNIFESP] MacedoSoares, F. Amancio, Olga Maria Silverio [UNIFESP] Jancar, Sonia [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) INST BUTANTAN |
dc.contributor.author.fl_str_mv |
Cunha, M. G. Naspitz, Charles Kirov [UNIFESP] MacedoSoares, F. Amancio, Olga Maria Silverio [UNIFESP] Jancar, Sonia [UNIFESP] |
dc.subject.eng.fl_str_mv |
malnutrition lung allergy vascular permeability rat airways protein-calorie deficiency |
topic |
malnutrition lung allergy vascular permeability rat airways protein-calorie deficiency |
description |
Background Although it is commonly accepted that the immune response is affected by malnutrition there are very few data about its effect in allergic diseases.Objective The aim of the present study was to investigate the effect of malnutrition in allergic lung inflammation.Methods An anaphylactic reaction was induced in rat lungs and the increased vascular permeability was measured in the trachea, internal and external bronchi and parenchyma by the Evans blue extravasation method. These studies were conducted in two dietary groups: one fed a normoproteic diet (18%) and the other a hypoproteic diet (4.5%). When the animals were 60 days old the group fed the hypoproteic diet presented a reduction of 77.86% in bodyweight, 63.3% in food intake and 36% in plasma protein concentration characterizing a severe protein-calorie malnutrition.Results The anaphylactic reaction in the lungs induced a significant increase in vascular permeability in the trachea and bronchi of both dietary groups. However, the intensity of this effect was significantly lower in the malnourished group. Analysis of immunoglobulin isotypes in the serum by ELISA showed that whereas IgG1 and IgG2a levels were similar in both groups, the levels of IgE were significantly lower in the malnourished animals. Moreover, the levels of antigen-specific IgG1, IgG2a and IgE were all significantly inhibited by the protein-calorie malnutrition. When antibodies were passively transfered to the malnourished rats, they developed a reaction as intense as the normoproteic group.Conclusion These results suggest that the capacity to release inflammatory mediators and the vascular response to these mediators is not affected by this type of malnutrition and, therefore, the diminished response of the airways reported here is probably due to the lower levels of anaphylactic antibodies produced by the malnourished rats. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997-10-01 |
dc.date.accessioned.fl_str_mv |
2018-06-15T16:39:18Z |
dc.date.available.fl_str_mv |
2018-06-15T16:39:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinical And Experimental Allergy. Oxford: Blackwell Science Ltd, v. 27, n. 10, p. 1212-1218, 1997. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/43281 http://dx.doi.org/10.1111/j.1365-2222.1997.tb01159.x |
dc.identifier.issn.none.fl_str_mv |
0954-7894 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1365-2222.1997.tb01159.x |
dc.identifier.wos.none.fl_str_mv |
WOS:A1997YB77500014 |
identifier_str_mv |
Clinical And Experimental Allergy. Oxford: Blackwell Science Ltd, v. 27, n. 10, p. 1212-1218, 1997. 0954-7894 10.1111/j.1365-2222.1997.tb01159.x WOS:A1997YB77500014 |
url |
http://repositorio.unifesp.br/11600/43281 http://dx.doi.org/10.1111/j.1365-2222.1997.tb01159.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Clinical And Experimental Allergy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1212-1218 |
dc.publisher.none.fl_str_mv |
Blackwell Science Ltd |
publisher.none.fl_str_mv |
Blackwell Science Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460257164427264 |