Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35420 http://dx.doi.org/10.1016/j.foodres.2012.07.010 |
Resumo: | Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved. |
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Omena, Cristhiane Maria BazilioValentim, Iara BarrosGuedes, Glaucevane da SilvaRabelo, Luiza AntasMano, Camila MarinhoEtelvino, José Henriques Bechara, [UNIFESP]Sawaya, Alexandra Christine Helena FranklandTrevisan, Maria Teresa SallesCosta, Joao Gomes daFerreira, Roberta Costa SilvaSant'Ana, Antonio Euzebio GoulartGoulart, Marilia Oliveira FonsecaUniv Fed AlagoasUniv PernambucoUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Univ Fed CearaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)2016-01-24T14:27:54Z2016-01-24T14:27:54Z2012-11-01Food Research International. Amsterdam: Elsevier B.V., v. 49, n. 1, p. 334-344, 2012.0963-9969http://repositorio.unifesp.br/handle/11600/35420http://dx.doi.org/10.1016/j.foodres.2012.07.010WOS000311592500043.pdf10.1016/j.foodres.2012.07.010WOS:000311592500043Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)INCT-RedoxomaINCT-BioanaliticaUniv Fed Alagoas, Inst Quim & Biotecnol, BR-57072970 Maceio, AL, BrazilUniv Pernambuco, BR-56300000 Petrolina, PE, BrazilUniv Fed Alagoas, Rede Nordeste Biotecnol RENORBIO, BR-57072970 Maceio, AL, BrazilUniv Fed Alagoas, Inst Ciencias Biol & Saude, BR-57072970 Maceio, AL, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508000 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Biol Vegetal, Campinas, SP, BrazilUniv Fed Ceara, Dept Quim Organ & Inorgan, BR-60021970 Fortaleza, CE, BrazilEmbrapa Tabuleiros Costeiros, Tabuleiro Martins, BR-57061970 Maceio, AL, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, BrazilFAPESP: 2008/58035-6Web of Science334-344engElsevier B.V.Food Research Internationalhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessTotal phenolic contentFree radical scavenging activityAntioxidant capacityBODIPYAcetylcholinesterase inhibitionTropical fruitsAntioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruitsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000311592500043.pdfapplication/pdf938547${dspace.ui.url}/bitstream/11600/35420/1/WOS000311592500043.pdf93952359d119b45c11c631c396690dfaMD51open accessTEXTWOS000311592500043.pdf.txtWOS000311592500043.pdf.txtExtracted texttext/plain71861${dspace.ui.url}/bitstream/11600/35420/2/WOS000311592500043.pdf.txtc3352a1ce587644faa834178ef875c13MD52open access11600/354202017-01-19 08:54:05.398open accessoai:repositorio.unifesp.br:11600/35420Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652017-01-19T10:54:05Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
title |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
spellingShingle |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits Omena, Cristhiane Maria Bazilio Total phenolic content Free radical scavenging activity Antioxidant capacity BODIPY Acetylcholinesterase inhibition Tropical fruits |
title_short |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
title_full |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
title_fullStr |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
title_full_unstemmed |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
title_sort |
Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits |
author |
Omena, Cristhiane Maria Bazilio |
author_facet |
Omena, Cristhiane Maria Bazilio Valentim, Iara Barros Guedes, Glaucevane da Silva Rabelo, Luiza Antas Mano, Camila Marinho Etelvino, José Henriques Bechara, [UNIFESP] Sawaya, Alexandra Christine Helena Frankland Trevisan, Maria Teresa Salles Costa, Joao Gomes da Ferreira, Roberta Costa Silva Sant'Ana, Antonio Euzebio Goulart Goulart, Marilia Oliveira Fonseca |
author_role |
author |
author2 |
Valentim, Iara Barros Guedes, Glaucevane da Silva Rabelo, Luiza Antas Mano, Camila Marinho Etelvino, José Henriques Bechara, [UNIFESP] Sawaya, Alexandra Christine Helena Frankland Trevisan, Maria Teresa Salles Costa, Joao Gomes da Ferreira, Roberta Costa Silva Sant'Ana, Antonio Euzebio Goulart Goulart, Marilia Oliveira Fonseca |
author2_role |
author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Fed Alagoas Univ Pernambuco Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Campinas (UNICAMP) Univ Fed Ceara Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Omena, Cristhiane Maria Bazilio Valentim, Iara Barros Guedes, Glaucevane da Silva Rabelo, Luiza Antas Mano, Camila Marinho Etelvino, José Henriques Bechara, [UNIFESP] Sawaya, Alexandra Christine Helena Frankland Trevisan, Maria Teresa Salles Costa, Joao Gomes da Ferreira, Roberta Costa Silva Sant'Ana, Antonio Euzebio Goulart Goulart, Marilia Oliveira Fonseca |
dc.subject.eng.fl_str_mv |
Total phenolic content Free radical scavenging activity Antioxidant capacity BODIPY Acetylcholinesterase inhibition Tropical fruits |
topic |
Total phenolic content Free radical scavenging activity Antioxidant capacity BODIPY Acetylcholinesterase inhibition Tropical fruits |
description |
Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-11-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:27:54Z |
dc.date.available.fl_str_mv |
2016-01-24T14:27:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Food Research International. Amsterdam: Elsevier B.V., v. 49, n. 1, p. 334-344, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/35420 http://dx.doi.org/10.1016/j.foodres.2012.07.010 |
dc.identifier.issn.none.fl_str_mv |
0963-9969 |
dc.identifier.file.none.fl_str_mv |
WOS000311592500043.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.foodres.2012.07.010 |
dc.identifier.wos.none.fl_str_mv |
WOS:000311592500043 |
identifier_str_mv |
Food Research International. Amsterdam: Elsevier B.V., v. 49, n. 1, p. 334-344, 2012. 0963-9969 WOS000311592500043.pdf 10.1016/j.foodres.2012.07.010 WOS:000311592500043 |
url |
http://repositorio.unifesp.br/handle/11600/35420 http://dx.doi.org/10.1016/j.foodres.2012.07.010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Food Research International |
dc.rights.driver.fl_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
334-344 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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