High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media

Detalhes bibliográficos
Autor(a) principal: Motta, Eduardo Leme Alves da [UNIFESP]
Data de Publicação: 1998
Outros Autores: Alegretti, Jose Roberto [UNIFESP], Baracat, Edmund Chada [UNIFESP], Olive, David, Serafini, Paulo Cesar [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://doi.org/10.1016/S0015-0282(98)00263-5
http://repositorio.unifesp.br/handle/11600/43683
Resumo: Objective: To evaluate the proficiency of preimplantation stage one (P1) and blastocyst media in supporting human blastocyst development and to document implantation and clinical pregnancy rates from the transfer of the normally developed blastocysts.Design: Retrospective clinical study.Setting: Private IVF unit of a university-affiliate center.Patient(s): Twenty-eight women aged 33.7 +/- 2.9 years who underwent IVF treatment for infertility.Intervention(s): Bipronucleate oocytes obtained from IVF and intracytoplasmic sperm injection were cultured in vitro with P1 and blastocyst media for 96-120 hours. One to three embryos were transferred (2.1 +/- 0.2 for the patients who became pregnant and 1.5 +/- 0.3 for those who did not become pregnant).Main Outcome Measure(s): Total number and percentage of developed blastocysts, frequency of blastocysts of grades A and B, and implantation and pregnancy rates. Result(s): From 431 oocytes retrieved, 269 bipronucleate oocytes were cultured, producing 81 blastocysts that resulted in the transfer of 54 embryos in 27 procedures. Blastocysts developed in 39.7% +/- 5.5% of the pregnant group and 30.2% +/- 4.5% of the nonpregnant group. From 15 (15/27 = 55.6%) clinical pregnancies, 18 (18/54 = 33.3%) gestational sacs were visualized. The rate of implantation in the pregnant group was 58.1% (18/31).Conclusion(s): These results provide evidence for the benefits of extending human embryo culture with P1 and blastocyst media for all normally fertilized embryos in vitro. (Fertil Steril(R) 1998;70:659-63. (C) 1998 by American Society for Reproductive Medicine.).
id UFSP_ec1477cf63540de9ceb037a48a2ac0f0
oai_identifier_str oai:repositorio.unifesp.br/:11600/43683
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst mediablastocystin vitro embryo developmentblastocyst mediumembryo transferimplantation ratespregnancy ratesObjective: To evaluate the proficiency of preimplantation stage one (P1) and blastocyst media in supporting human blastocyst development and to document implantation and clinical pregnancy rates from the transfer of the normally developed blastocysts.Design: Retrospective clinical study.Setting: Private IVF unit of a university-affiliate center.Patient(s): Twenty-eight women aged 33.7 +/- 2.9 years who underwent IVF treatment for infertility.Intervention(s): Bipronucleate oocytes obtained from IVF and intracytoplasmic sperm injection were cultured in vitro with P1 and blastocyst media for 96-120 hours. One to three embryos were transferred (2.1 +/- 0.2 for the patients who became pregnant and 1.5 +/- 0.3 for those who did not become pregnant).Main Outcome Measure(s): Total number and percentage of developed blastocysts, frequency of blastocysts of grades A and B, and implantation and pregnancy rates. Result(s): From 431 oocytes retrieved, 269 bipronucleate oocytes were cultured, producing 81 blastocysts that resulted in the transfer of 54 embryos in 27 procedures. Blastocysts developed in 39.7% +/- 5.5% of the pregnant group and 30.2% +/- 4.5% of the nonpregnant group. From 15 (15/27 = 55.6%) clinical pregnancies, 18 (18/54 = 33.3%) gestational sacs were visualized. The rate of implantation in the pregnant group was 58.1% (18/31).Conclusion(s): These results provide evidence for the benefits of extending human embryo culture with P1 and blastocyst media for all normally fertilized embryos in vitro. (Fertil Steril(R) 1998;70:659-63. (C) 1998 by American Society for Reproductive Medicine.).Ctr Med Reprod Huntington Sao Paulo, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Div Gynecol Endocrinol, Sao Paulo, BrazilYale Univ, Sch Med, Dept Obstet & Gynecol, New Haven, CT 06510 USAUniv Fed Sao Paulo, Escola Paulista Med, Div Gynecol Endocrinol, Sao Paulo, BrazilWeb of ScienceAmer Soc Reproductive MedicineCtr Med Reprod Huntington Sao PauloUniversidade Federal de São Paulo (UNIFESP)Yale UnivMotta, Eduardo Leme Alves da [UNIFESP]Alegretti, Jose Roberto [UNIFESP]Baracat, Edmund Chada [UNIFESP]Olive, DavidSerafini, Paulo Cesar [UNIFESP]2018-06-15T17:30:17Z2018-06-15T17:30:17Z1998-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion659-663https://doi.org/10.1016/S0015-0282(98)00263-5Fertility And Sterility. Birmingham: Amer Soc Reproductive Medicine, v. 70, n. 4, p. 659-663, 1998.10.1016/S0015-0282(98)00263-50015-0282http://repositorio.unifesp.br/handle/11600/43683WOS:000076505400009engFertility And Sterilityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T15:52:31Zoai:repositorio.unifesp.br/:11600/43683Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T15:52:31Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
title High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
spellingShingle High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
Motta, Eduardo Leme Alves da [UNIFESP]
blastocyst
in vitro embryo development
blastocyst medium
embryo transfer
implantation rates
pregnancy rates
title_short High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
title_full High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
title_fullStr High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
title_full_unstemmed High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
title_sort High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
author Motta, Eduardo Leme Alves da [UNIFESP]
author_facet Motta, Eduardo Leme Alves da [UNIFESP]
Alegretti, Jose Roberto [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
Olive, David
Serafini, Paulo Cesar [UNIFESP]
author_role author
author2 Alegretti, Jose Roberto [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
Olive, David
Serafini, Paulo Cesar [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ctr Med Reprod Huntington Sao Paulo
Universidade Federal de São Paulo (UNIFESP)
Yale Univ
dc.contributor.author.fl_str_mv Motta, Eduardo Leme Alves da [UNIFESP]
Alegretti, Jose Roberto [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
Olive, David
Serafini, Paulo Cesar [UNIFESP]
dc.subject.por.fl_str_mv blastocyst
in vitro embryo development
blastocyst medium
embryo transfer
implantation rates
pregnancy rates
topic blastocyst
in vitro embryo development
blastocyst medium
embryo transfer
implantation rates
pregnancy rates
description Objective: To evaluate the proficiency of preimplantation stage one (P1) and blastocyst media in supporting human blastocyst development and to document implantation and clinical pregnancy rates from the transfer of the normally developed blastocysts.Design: Retrospective clinical study.Setting: Private IVF unit of a university-affiliate center.Patient(s): Twenty-eight women aged 33.7 +/- 2.9 years who underwent IVF treatment for infertility.Intervention(s): Bipronucleate oocytes obtained from IVF and intracytoplasmic sperm injection were cultured in vitro with P1 and blastocyst media for 96-120 hours. One to three embryos were transferred (2.1 +/- 0.2 for the patients who became pregnant and 1.5 +/- 0.3 for those who did not become pregnant).Main Outcome Measure(s): Total number and percentage of developed blastocysts, frequency of blastocysts of grades A and B, and implantation and pregnancy rates. Result(s): From 431 oocytes retrieved, 269 bipronucleate oocytes were cultured, producing 81 blastocysts that resulted in the transfer of 54 embryos in 27 procedures. Blastocysts developed in 39.7% +/- 5.5% of the pregnant group and 30.2% +/- 4.5% of the nonpregnant group. From 15 (15/27 = 55.6%) clinical pregnancies, 18 (18/54 = 33.3%) gestational sacs were visualized. The rate of implantation in the pregnant group was 58.1% (18/31).Conclusion(s): These results provide evidence for the benefits of extending human embryo culture with P1 and blastocyst media for all normally fertilized embryos in vitro. (Fertil Steril(R) 1998;70:659-63. (C) 1998 by American Society for Reproductive Medicine.).
publishDate 1998
dc.date.none.fl_str_mv 1998-10-01
2018-06-15T17:30:17Z
2018-06-15T17:30:17Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/S0015-0282(98)00263-5
Fertility And Sterility. Birmingham: Amer Soc Reproductive Medicine, v. 70, n. 4, p. 659-663, 1998.
10.1016/S0015-0282(98)00263-5
0015-0282
http://repositorio.unifesp.br/handle/11600/43683
WOS:000076505400009
url https://doi.org/10.1016/S0015-0282(98)00263-5
http://repositorio.unifesp.br/handle/11600/43683
identifier_str_mv Fertility And Sterility. Birmingham: Amer Soc Reproductive Medicine, v. 70, n. 4, p. 659-663, 1998.
10.1016/S0015-0282(98)00263-5
0015-0282
WOS:000076505400009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fertility And Sterility
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 659-663
dc.publisher.none.fl_str_mv Amer Soc Reproductive Medicine
publisher.none.fl_str_mv Amer Soc Reproductive Medicine
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268451871522816