Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats
Main Author: | |
---|---|
Publication Date: | 2017 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNIFESP |
Download full: | https://repositorio.unifesp.br/handle/11600/57374 https://dx.doi.org/10.3389/fphar.2017.00605 |
Summary: | Menopause is associated with increased risk to develop obesity but the mechanisms involved are not fully understood. We have shown that Ginkgo biloba extract (GbE) improved diet-induced obesity. Since GbE might be effective in the treatment of obesity related to menopause, avoiding the side effects of hormone replacement therapy, we investigated the effect of GbE on hypothalamic systems controlling energy homeostasis. Wistar rats were either ovariectomized (OVX) or Sham-operated. After 2 months, either 500 mg.kg(-1) of GbE or vehicle were administered daily by gavage for 14 days. A subset of animals received an intracerebroventricular (i.c.v.) injection of serotonin (300 mu g) or vehicle and food intake was measured after 12 and 24 h. Another subset was submitted to in vivo microdialysis and 5-HT levels of the medial hypothalamus were measured by high performance liquid chromatography, before and up to 2 h after the administration of 500 mg.kg(-1) of GbE. Additional animals were used for quantification of 5-HT1A, 5-HT1B, 5-HT2C, 5-HTT, and pro-opiomelanocortin hypothalamic protein levels by Western blotting. OVX increased food intake and body weight and adiposity while GbE attenuated these alterations. i.c.v. serotonin significantly reduced food intake in Sham, Sham + GbE, and OVX + GbE groups while it failed to do so in the OVX group. In the OVX rats, GbE stimulated 5-HT microdialysate levels while it reduced hypothalamic 5-HTT protein levels. The results indicate that GbE improved the ovariectomy-induced resistance to serotonin hypophagia, at least in part through stimulation of the hypothalamic serotonergic activity. Since body weight gain is one of the most important consequences of menopause, the stimulation of the serotonergic transmission by GbE may represent a potential alternative therapy for menopause-related obesity. |
id |
UFSP_ed845de5a719c8725772a543420e3796 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/57374 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Banin, Renata Mancini [UNIFESP]Andrade, Iracema Senna de [UNIFESP]Cerutti, Suzete Maria [UNIFESP]Oyama, Lila Missae [UNIFESP]Telles, Monica Marques [UNIFESP]Ribeiro, Eliane Beraldi [UNIFESP]2020-08-04T13:40:13Z2020-08-04T13:40:13Z2017Frontiers In Pharmacology. Lausanne, v. 8, p. -, 2017.1663-9812https://repositorio.unifesp.br/handle/11600/57374https://dx.doi.org/10.3389/fphar.2017.00605WOS000409382600001.pdf10.3389/fphar.2017.00605WOS:000409382600001Menopause is associated with increased risk to develop obesity but the mechanisms involved are not fully understood. We have shown that Ginkgo biloba extract (GbE) improved diet-induced obesity. Since GbE might be effective in the treatment of obesity related to menopause, avoiding the side effects of hormone replacement therapy, we investigated the effect of GbE on hypothalamic systems controlling energy homeostasis. Wistar rats were either ovariectomized (OVX) or Sham-operated. After 2 months, either 500 mg.kg(-1) of GbE or vehicle were administered daily by gavage for 14 days. A subset of animals received an intracerebroventricular (i.c.v.) injection of serotonin (300 mu g) or vehicle and food intake was measured after 12 and 24 h. Another subset was submitted to in vivo microdialysis and 5-HT levels of the medial hypothalamus were measured by high performance liquid chromatography, before and up to 2 h after the administration of 500 mg.kg(-1) of GbE. Additional animals were used for quantification of 5-HT1A, 5-HT1B, 5-HT2C, 5-HTT, and pro-opiomelanocortin hypothalamic protein levels by Western blotting. OVX increased food intake and body weight and adiposity while GbE attenuated these alterations. i.c.v. serotonin significantly reduced food intake in Sham, Sham + GbE, and OVX + GbE groups while it failed to do so in the OVX group. In the OVX rats, GbE stimulated 5-HT microdialysate levels while it reduced hypothalamic 5-HTT protein levels. The results indicate that GbE improved the ovariectomy-induced resistance to serotonin hypophagia, at least in part through stimulation of the hypothalamic serotonergic activity. Since body weight gain is one of the most important consequences of menopause, the stimulation of the serotonergic transmission by GbE may represent a potential alternative therapy for menopause-related obesity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Biol, Setor Morfofisiol & Patol, Diadema, BrazilUniv Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Biol, Setor Morfofisiol & Patol, Diadema, BrazilCNPq: 453924/2014-0FAPESP: 2012/03172-4FAPESP: 2014/18435-6Web of Science-engFrontiers Media SaFrontiers In PharmacologyOvariectomyObesitySerotoninHypothalamusFood intakein vivo microdialysisGinkgo biloba extractGinkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLausanne8info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000409382600001.pdfapplication/pdf1947027${dspace.ui.url}/bitstream/11600/57374/1/WOS000409382600001.pdfe778c3cbf53ede8bbfab682193bd414cMD51open accessTEXTWOS000409382600001.pdf.txtWOS000409382600001.pdf.txtExtracted texttext/plain52332${dspace.ui.url}/bitstream/11600/57374/8/WOS000409382600001.pdf.txt824dd4347f1abe2b99657f3cc87042aaMD58open accessTHUMBNAILWOS000409382600001.pdf.jpgWOS000409382600001.pdf.jpgIM Thumbnailimage/jpeg7217${dspace.ui.url}/bitstream/11600/57374/10/WOS000409382600001.pdf.jpg723dfa89e6f281ddc5da53b7f0040745MD510open access11600/573742023-06-05 19:33:03.553open accessoai:repositorio.unifesp.br:11600/57374Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:33:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
title |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
spellingShingle |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats Banin, Renata Mancini [UNIFESP] Ovariectomy Obesity Serotonin Hypothalamus Food intake in vivo microdialysis Ginkgo biloba extract |
title_short |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
title_full |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
title_fullStr |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
title_full_unstemmed |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
title_sort |
Ginkgo biloba Extract (GbE) Stimulates the Hypothalamic Serotonergic System and Attenuates Obesity in Ovariectomized Rats |
author |
Banin, Renata Mancini [UNIFESP] |
author_facet |
Banin, Renata Mancini [UNIFESP] Andrade, Iracema Senna de [UNIFESP] Cerutti, Suzete Maria [UNIFESP] Oyama, Lila Missae [UNIFESP] Telles, Monica Marques [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] |
author_role |
author |
author2 |
Andrade, Iracema Senna de [UNIFESP] Cerutti, Suzete Maria [UNIFESP] Oyama, Lila Missae [UNIFESP] Telles, Monica Marques [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Banin, Renata Mancini [UNIFESP] Andrade, Iracema Senna de [UNIFESP] Cerutti, Suzete Maria [UNIFESP] Oyama, Lila Missae [UNIFESP] Telles, Monica Marques [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] |
dc.subject.eng.fl_str_mv |
Ovariectomy Obesity Serotonin Hypothalamus Food intake in vivo microdialysis Ginkgo biloba extract |
topic |
Ovariectomy Obesity Serotonin Hypothalamus Food intake in vivo microdialysis Ginkgo biloba extract |
description |
Menopause is associated with increased risk to develop obesity but the mechanisms involved are not fully understood. We have shown that Ginkgo biloba extract (GbE) improved diet-induced obesity. Since GbE might be effective in the treatment of obesity related to menopause, avoiding the side effects of hormone replacement therapy, we investigated the effect of GbE on hypothalamic systems controlling energy homeostasis. Wistar rats were either ovariectomized (OVX) or Sham-operated. After 2 months, either 500 mg.kg(-1) of GbE or vehicle were administered daily by gavage for 14 days. A subset of animals received an intracerebroventricular (i.c.v.) injection of serotonin (300 mu g) or vehicle and food intake was measured after 12 and 24 h. Another subset was submitted to in vivo microdialysis and 5-HT levels of the medial hypothalamus were measured by high performance liquid chromatography, before and up to 2 h after the administration of 500 mg.kg(-1) of GbE. Additional animals were used for quantification of 5-HT1A, 5-HT1B, 5-HT2C, 5-HTT, and pro-opiomelanocortin hypothalamic protein levels by Western blotting. OVX increased food intake and body weight and adiposity while GbE attenuated these alterations. i.c.v. serotonin significantly reduced food intake in Sham, Sham + GbE, and OVX + GbE groups while it failed to do so in the OVX group. In the OVX rats, GbE stimulated 5-HT microdialysate levels while it reduced hypothalamic 5-HTT protein levels. The results indicate that GbE improved the ovariectomy-induced resistance to serotonin hypophagia, at least in part through stimulation of the hypothalamic serotonergic activity. Since body weight gain is one of the most important consequences of menopause, the stimulation of the serotonergic transmission by GbE may represent a potential alternative therapy for menopause-related obesity. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-08-04T13:40:13Z |
dc.date.available.fl_str_mv |
2020-08-04T13:40:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Frontiers In Pharmacology. Lausanne, v. 8, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57374 https://dx.doi.org/10.3389/fphar.2017.00605 |
dc.identifier.issn.none.fl_str_mv |
1663-9812 |
dc.identifier.file.none.fl_str_mv |
WOS000409382600001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.3389/fphar.2017.00605 |
dc.identifier.wos.none.fl_str_mv |
WOS:000409382600001 |
identifier_str_mv |
Frontiers In Pharmacology. Lausanne, v. 8, p. -, 2017. 1663-9812 WOS000409382600001.pdf 10.3389/fphar.2017.00605 WOS:000409382600001 |
url |
https://repositorio.unifesp.br/handle/11600/57374 https://dx.doi.org/10.3389/fphar.2017.00605 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Frontiers In Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- |
dc.coverage.none.fl_str_mv |
Lausanne |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/57374/1/WOS000409382600001.pdf ${dspace.ui.url}/bitstream/11600/57374/8/WOS000409382600001.pdf.txt ${dspace.ui.url}/bitstream/11600/57374/10/WOS000409382600001.pdf.jpg |
bitstream.checksum.fl_str_mv |
e778c3cbf53ede8bbfab682193bd414c 824dd4347f1abe2b99657f3cc87042aa 723dfa89e6f281ddc5da53b7f0040745 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460343637344256 |