Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos

Detalhes bibliográficos
Autor(a) principal: Santos, Renata Gonçalves Martello dos [UNIFESP]
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5287243
http://repositorio.unifesp.br/handle/11600/49905
Resumo: Objective: To verify the presence of DNA polymorphisms rs2236479 (Col18A1) and rs2862296 (LOXL-4) and its association with POP in the Brazilian population. METHODS: 532 postmenopausal women divided into POP (stage III or IV by POP-Q) and control (stage 0 or I by POP-Q) were included. Women with previous hysterectomy or other pelvic surgery were excluded. The evaluation included clinical examination and blood collection. The genetic material was analyzed by RT-PCR. The statistical analysis of the data was performed according to the type of variable, using the chi-square test, mean and standard deviation, and median and 25th and 75th percentiles. For comparison between groups, the t test -Student for two independent samples and the Mann-Whitney test. The verification of the association between genotypes and the presence of prolapse was estimated by the odds ratio (OR) and respective 95% confidence intervals (95% CI) using binary logistic regression models. Results: A greater proportion of the genotypes with at least one polymorphic allele (AG and GG) of the LOXL-4 gene was verified in the control group compared to the POP group (73.2% and 67.3%, respectively, p = 0.465 ). As for the Col8A1 gene, a higher percentage of GA genotype was found in the control group (50.2% and 44.2%, respectively) and a higher number of women with POP and both polymorphic alleles (AA) (17.5% and 15.4% respectively), non-statistically significant results (p = 0.556). No associations were observed between presence of polymorphisms and POP. However, in the group of women homozygous for the polymorphic allele, both for LOXL-4 (GG) and Col18A1 (AA), OR less than 1 (OR = 0.84, 95% CI (0.47, 1.50 ) and (OR = 0.91, 95% CI (0.50, 1.65), respectively, suggest that these genotypes may be protective factors for POPs. In addition, the variables age, higher number of pregnancies, normal and presence of family history of POP were associated with the presence of dysfunction. Conclusion: There was no association between the presence of polymorphisms rs2862296, of the LOXL-4 gene, and rs2236479, of the Col18A1 mole, and the development of genital prolapse degrees III or IV in this sample of patients.
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spelling Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicosDNA polymorphisms in women with and without advanced pelvic organ prolapseEstudo de polimorfismos de DNA em mulheres com e sem prolapso acentuado de órgãos pélvicosPelvic organPolymorphismCollagenExtracelular matrixPelvic organ prolapseProlapso de orgãos pélvicosPolimorfismoColágenoObjective: To verify the presence of DNA polymorphisms rs2236479 (Col18A1) and rs2862296 (LOXL-4) and its association with POP in the Brazilian population. METHODS: 532 postmenopausal women divided into POP (stage III or IV by POP-Q) and control (stage 0 or I by POP-Q) were included. Women with previous hysterectomy or other pelvic surgery were excluded. The evaluation included clinical examination and blood collection. The genetic material was analyzed by RT-PCR. The statistical analysis of the data was performed according to the type of variable, using the chi-square test, mean and standard deviation, and median and 25th and 75th percentiles. For comparison between groups, the t test -Student for two independent samples and the Mann-Whitney test. The verification of the association between genotypes and the presence of prolapse was estimated by the odds ratio (OR) and respective 95% confidence intervals (95% CI) using binary logistic regression models. Results: A greater proportion of the genotypes with at least one polymorphic allele (AG and GG) of the LOXL-4 gene was verified in the control group compared to the POP group (73.2% and 67.3%, respectively, p = 0.465 ). As for the Col8A1 gene, a higher percentage of GA genotype was found in the control group (50.2% and 44.2%, respectively) and a higher number of women with POP and both polymorphic alleles (AA) (17.5% and 15.4% respectively), non-statistically significant results (p = 0.556). No associations were observed between presence of polymorphisms and POP. However, in the group of women homozygous for the polymorphic allele, both for LOXL-4 (GG) and Col18A1 (AA), OR less than 1 (OR = 0.84, 95% CI (0.47, 1.50 ) and (OR = 0.91, 95% CI (0.50, 1.65), respectively, suggest that these genotypes may be protective factors for POPs. In addition, the variables age, higher number of pregnancies, normal and presence of family history of POP were associated with the presence of dysfunction. Conclusion: There was no association between the presence of polymorphisms rs2862296, of the LOXL-4 gene, and rs2236479, of the Col18A1 mole, and the development of genital prolapse degrees III or IV in this sample of patients.Objetivo: Verificar a presença dos polimorfismos de DNA rs2236479 (Col18A1) e rs2862296(LOXL-4) e sua associação com POP na população Brasileira. Métodos: Foram incluídas 532 mulheres pós-menopáusicas divididas em grupos POP(estádio III ou IV por POP-Q) e controle (estádios 0 ou I por POP-Q). Foram excluídas as mulheres com histerectomia anterior ou outras cirurgia pélvicas. A avaliação incluiu exame clínico e coleta de sangue. O material genético foi analisado por RT-PCR. A análise estatística dos dados foi realizada, de acordo com o tipo de variável, utilizando o teste o qui-quadrado, média e desvio-padrão, e mediana e percentis 25 e 75. Para comparação entre os grupos, utilizou-se o teste t-Student para duas amostras independentes e o teste de Mann-Whitney. A verificação da associação entre genótipos e a presença de prolapso foi estimada pelo odds ratio (OR) e respectivos intervalos de confiança a 95% (IC 95%) utilizando modelos de regressão logística binária. Resultados: Verificou-se maior proporção dos genótipos com ao menos um alelo polimórfico (AG e GG) do gene da LOXL-4 no grupo controle em relação ao grupo POP (73,2% e 67,3%,respectivamente, p=0,465). Quanto ao gene Col8A1, verificou-se um maior percentual de genótipo GA no grupo controle (50,2% e 44,2% respectivamente) e maior número de mulheres com POP e ambos os alelos polimórficos (AA) (17,5% e 15,4% respectivamente), resultados não estatisticamente significantes (p=0,556). Não observadas associações entre presença dos polimorfismos e POP. Porém, no grupo de mulheres homozigotas para o alelo polimórfico, tanto para a LOXL-4 (GG) como o Col18A1 (AA), OR inferiores a 1 (OR=0,84, IC 95% (0,47; 1,50) e (OR=0,91, IC 95% (0,50; 1,65), respectivamente sugerem que esse genótipos possam ser fatores protetores para POP. Além disso, as variáveis idade, maior número de gestações, maior número de partos normais e presença de história familiar de POP mostraram-se associadas à presença da disfunção. Conclusão: Não houve associação entre a presença dos polimorfismos rs2862296, do gene da LOXL-4, e rs2236479, do geme Col18A1, e o desenvolvimento de prolapso genital graus III ou IV nessa amostragem de pacientes.Dados abertos - Sucupira - Teses e dissertações (2017)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo (UNIFESP)Castro, Rodrigo de Aquino [UNIFESP]Bortolini, Maria Augusta Tezelli [UNIFESP]Carvalho, Cristina Valletta de [UNIFESP]Maria Augusta Tezelli Bortolini : http://lattes.cnpq.br/1150368284144393Cristina Valletta de Carvalho : http://lattes.cnpq.br/6006653389006277http://lattes.cnpq.br/6590913930590292http://lattes.cnpq.br/5544814435283297Universidade Federal de São Paulo (UNIFESP)Santos, Renata Gonçalves Martello dos [UNIFESP]2019-06-19T14:57:05Z2019-06-19T14:57:05Z2017-05-30info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion77 f.application/pdfhttps://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5287243http://repositorio.unifesp.br/handle/11600/49905porSão Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-10T10:09:13Zoai:repositorio.unifesp.br/:11600/49905Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-10T10:09:13Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
DNA polymorphisms in women with and without advanced pelvic organ prolapse
Estudo de polimorfismos de DNA em mulheres com e sem prolapso acentuado de órgãos pélvicos
title Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
spellingShingle Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
Santos, Renata Gonçalves Martello dos [UNIFESP]
Pelvic organ
Polymorphism
Collagen
Extracelular matrix
Pelvic organ prolapse
Prolapso de orgãos pélvicos
Polimorfismo
Colágeno
title_short Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
title_full Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
title_fullStr Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
title_full_unstemmed Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
title_sort Estudo de polimorfismos de DNA em mulheres com e sem prolapso avançado de órgãos pélvicos
author Santos, Renata Gonçalves Martello dos [UNIFESP]
author_facet Santos, Renata Gonçalves Martello dos [UNIFESP]
author_role author
dc.contributor.none.fl_str_mv Castro, Rodrigo de Aquino [UNIFESP]
Bortolini, Maria Augusta Tezelli [UNIFESP]
Carvalho, Cristina Valletta de [UNIFESP]
Maria Augusta Tezelli Bortolini : http://lattes.cnpq.br/1150368284144393
Cristina Valletta de Carvalho : http://lattes.cnpq.br/6006653389006277
http://lattes.cnpq.br/6590913930590292
http://lattes.cnpq.br/5544814435283297
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Santos, Renata Gonçalves Martello dos [UNIFESP]
dc.subject.por.fl_str_mv Pelvic organ
Polymorphism
Collagen
Extracelular matrix
Pelvic organ prolapse
Prolapso de orgãos pélvicos
Polimorfismo
Colágeno
topic Pelvic organ
Polymorphism
Collagen
Extracelular matrix
Pelvic organ prolapse
Prolapso de orgãos pélvicos
Polimorfismo
Colágeno
description Objective: To verify the presence of DNA polymorphisms rs2236479 (Col18A1) and rs2862296 (LOXL-4) and its association with POP in the Brazilian population. METHODS: 532 postmenopausal women divided into POP (stage III or IV by POP-Q) and control (stage 0 or I by POP-Q) were included. Women with previous hysterectomy or other pelvic surgery were excluded. The evaluation included clinical examination and blood collection. The genetic material was analyzed by RT-PCR. The statistical analysis of the data was performed according to the type of variable, using the chi-square test, mean and standard deviation, and median and 25th and 75th percentiles. For comparison between groups, the t test -Student for two independent samples and the Mann-Whitney test. The verification of the association between genotypes and the presence of prolapse was estimated by the odds ratio (OR) and respective 95% confidence intervals (95% CI) using binary logistic regression models. Results: A greater proportion of the genotypes with at least one polymorphic allele (AG and GG) of the LOXL-4 gene was verified in the control group compared to the POP group (73.2% and 67.3%, respectively, p = 0.465 ). As for the Col8A1 gene, a higher percentage of GA genotype was found in the control group (50.2% and 44.2%, respectively) and a higher number of women with POP and both polymorphic alleles (AA) (17.5% and 15.4% respectively), non-statistically significant results (p = 0.556). No associations were observed between presence of polymorphisms and POP. However, in the group of women homozygous for the polymorphic allele, both for LOXL-4 (GG) and Col18A1 (AA), OR less than 1 (OR = 0.84, 95% CI (0.47, 1.50 ) and (OR = 0.91, 95% CI (0.50, 1.65), respectively, suggest that these genotypes may be protective factors for POPs. In addition, the variables age, higher number of pregnancies, normal and presence of family history of POP were associated with the presence of dysfunction. Conclusion: There was no association between the presence of polymorphisms rs2862296, of the LOXL-4 gene, and rs2236479, of the Col18A1 mole, and the development of genital prolapse degrees III or IV in this sample of patients.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-30
2019-06-19T14:57:05Z
2019-06-19T14:57:05Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5287243
http://repositorio.unifesp.br/handle/11600/49905
url https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5287243
http://repositorio.unifesp.br/handle/11600/49905
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 77 f.
application/pdf
dc.coverage.none.fl_str_mv São Paulo
dc.publisher.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
publisher.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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