CLL: chromosomal abnormalities (FISH) and their relation with clinical stage, CD38 and ZAP-70

• Autor(a) principal: Nascimento, Marilia C. [UNIFESP]
• Data de Publicação: 2006
• Outros Autores: Yamamoto, Mihoko [UNIFESP], Rodrigues, Maria Madalena [UNIFESP], Franco, Luciana F. [UNIFESP], Kimura, Elisa Y. S. [UNIFESP], Vasconcelos, Yuri [UNIFESP], Oliveira, José Salvador Rodrigues de [UNIFESP], Figueiredo, Vera L. P. [UNIFESP], Chauffaille, Maria de Lourdes Lopes Ferrari [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300000954t
• Texto Completo: http://dx.doi.org/10.1590/S1516-84842006000100004; http://repositorio.unifesp.br/handle/11600/2955
• topic: Chronic lymphocytic leukemia; FISH; cytogenetics; Leucemia linfocítica crônica; FISH; citogenética

Chronic lymphocytic leukemia is the most prevalent type of leukemia in the West. It is characterized by an extremely variable clinical course. The aim of the study was to detect the most frequent chromosomal abnormalities in patients with CLL using FISH, and assess them regarding age, gender, clinical stage and CD38 and ZAP-70 expressions. We found 51.7% of the patients with chromosome abnormalities. The most frequent one was del 13q14 in 34.5% of cases. It was associated to other alterations in 17.2%. 17p13 deletions were found in 17.2% and trisomy 12 in 13.8% (in isolation in 6.9% and associated to del 13q14, in 6.9% of the cases). An 11q22 deletion was found in one case associated to a 13q14 deletion. To better evaluate the relationship between chromosome aberrations and other prognostic factors in CLL, two cytogenetics groups were considered: favorable (13q deletion in isolation and no alteration) and unfavorable outcomes (trisomy 12, 17p13 deletion, 11q22 deletion and two simultaneous alterations).The unfavorable alterations were more frequently seen among young individuals (<60y). There were more females (70%) than males in this group (p=0.04). In relation to the Binet's staging system, patients with unfavorable cytogenetic alterations, tended to be B and C stages, while in the favorable group prevailed patients in stage A. Additionally, patients with poor prognostic cytogenetics tended to express CD38 and ZAP-70 proteins.