Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis?
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38364 http://dx.doi.org/10.1111/cei.12422 |
Resumo: | The objective of this study is to evaluate urinary high mobility group box 1 (HMGB1) levels as markers for active nephritis in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) in comparison with urinary CD4(+) effector memory T cells and urinary monocyte chemoattractant protein-1 (MCP-1). Twenty-four AAV patients with active nephritis and 12 healthy controls (HC) were evaluated. in nine patients, samples were also obtained during remission. Urinary levels of HMGB1 were measured by Western blot. CD4(+) T cells and CD4(+) effector memory T cells (CD4(+)CD45RO(+)CCR7(-)) were determined in urine and whole blood by flow cytometry. Measurement of urinary levels of MCP-1 and serum HMGB1 levels were performed by enzyme-linked immunosorbent assay (ELISA). AAV patients with active nephritis had higher median intensity of HMGB1 in urine than HC [10.3 (7.05-18.50) versus 5.8 (4.48-7.01); P = 0.004]. Both urinary HMGB1 and MCP-1 levels decreased significantly from active nephritis to remission. the urinary MCP-1/creatinine ratio correlated with Birmingham Vasculitis Activity Score (BVAS) (P = 0.042). No correlation was found between the HMGB1/creatinine ratio and 24-h proteinuria, estimated glomerular filtration rate (eGFR), MCP-1/creatinine ratio, BVAS and serum HMGB1. A positive correlation was found between urinary HMGB1/creatinine ratio and CD4(+) T cells/creatinine ratio (P = 0.028) and effector memory T cells/creatinine ratio (P = 0.039) in urine. Urinary HMGB1 levels are increased in AAV patients with active nephritis when compared with HC and patients in remission, and urinary HMGB1 levels are associated with CD4(+) T cells and CD4(+) effector memory T cells in urine. Measurement of urinary HMGB1 may be of additional value in identifying active glomerulonephritis in AAV patients. |
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Souza, A. W. S. de [UNIFESP]Abdulahad, W. H.Sosicka, P.Bijzet, J.Limburg, P. C.Stegeman, C. A.Bijl, M.Westra, J.Kallenberg, C. G. M.Univ GroningenMartini HospUniversidade Federal de São Paulo (UNIFESP)Univ Wroclaw2016-01-24T14:38:03Z2016-01-24T14:38:03Z2014-11-01Clinical and Experimental Immunology. Hoboken: Wiley-Blackwell, v. 178, n. 2, p. 270-278, 2014.0009-9104http://repositorio.unifesp.br/handle/11600/38364http://dx.doi.org/10.1111/cei.1242210.1111/cei.12422WOS:000342915800009The objective of this study is to evaluate urinary high mobility group box 1 (HMGB1) levels as markers for active nephritis in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) in comparison with urinary CD4(+) effector memory T cells and urinary monocyte chemoattractant protein-1 (MCP-1). Twenty-four AAV patients with active nephritis and 12 healthy controls (HC) were evaluated. in nine patients, samples were also obtained during remission. Urinary levels of HMGB1 were measured by Western blot. CD4(+) T cells and CD4(+) effector memory T cells (CD4(+)CD45RO(+)CCR7(-)) were determined in urine and whole blood by flow cytometry. Measurement of urinary levels of MCP-1 and serum HMGB1 levels were performed by enzyme-linked immunosorbent assay (ELISA). AAV patients with active nephritis had higher median intensity of HMGB1 in urine than HC [10.3 (7.05-18.50) versus 5.8 (4.48-7.01); P = 0.004]. Both urinary HMGB1 and MCP-1 levels decreased significantly from active nephritis to remission. the urinary MCP-1/creatinine ratio correlated with Birmingham Vasculitis Activity Score (BVAS) (P = 0.042). No correlation was found between the HMGB1/creatinine ratio and 24-h proteinuria, estimated glomerular filtration rate (eGFR), MCP-1/creatinine ratio, BVAS and serum HMGB1. A positive correlation was found between urinary HMGB1/creatinine ratio and CD4(+) T cells/creatinine ratio (P = 0.028) and effector memory T cells/creatinine ratio (P = 0.039) in urine. Urinary HMGB1 levels are increased in AAV patients with active nephritis when compared with HC and patients in remission, and urinary HMGB1 levels are associated with CD4(+) T cells and CD4(+) effector memory T cells in urine. Measurement of urinary HMGB1 may be of additional value in identifying active glomerulonephritis in AAV patients.Research Institute for Drug Exploration (GUIDE)Univ Groningen, Univ Med Ctr Groningen, Dept Rheumatol & Clin Immunol, NL-9713 GZ Groningen, NetherlandsUniv Groningen, Univ Med Ctr Groningen, Dept Lab Med, NL-9713 GZ Groningen, NetherlandsUniv Groningen, Univ Med Ctr Groningen, Dept Internal Med, Div Nephrol, NL-9713 GZ Groningen, NetherlandsMartini Hosp, Dept Internal Med & Rheumatol, Groningen, NetherlandsUniversidade Federal de São Paulo, Escola Paulista Med, Dept Internal Med, Div Rheumatol, São Paulo, BrazilUniv Wroclaw, Fac Biotechnol, Biochem Lab, PL-50138 Wroclaw, PolandUniversidade Federal de São Paulo, Escola Paulista Med, Dept Internal Med, Div Rheumatol, São Paulo, BrazilWeb of Science270-278engWiley-BlackwellClinical and Experimental Immunologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessanti-neutrophil cytoplasmic antibody-associated vasculitisglomerulonephritisHMGB1monocyte chemoattractant protein-1T cellsAre urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/383642022-02-18 10:09:41.221metadata only accessoai:repositorio.unifesp.br:11600/38364Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-18T13:09:41Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
title |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
spellingShingle |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? Souza, A. W. S. de [UNIFESP] anti-neutrophil cytoplasmic antibody-associated vasculitis glomerulonephritis HMGB1 monocyte chemoattractant protein-1 T cells |
title_short |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
title_full |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
title_fullStr |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
title_full_unstemmed |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
title_sort |
Are urinary levels of high mobility group box 1 markers of active nephritis in anti-neutrophil cytoplasmic antibody-associated vasculitis? |
author |
Souza, A. W. S. de [UNIFESP] |
author_facet |
Souza, A. W. S. de [UNIFESP] Abdulahad, W. H. Sosicka, P. Bijzet, J. Limburg, P. C. Stegeman, C. A. Bijl, M. Westra, J. Kallenberg, C. G. M. |
author_role |
author |
author2 |
Abdulahad, W. H. Sosicka, P. Bijzet, J. Limburg, P. C. Stegeman, C. A. Bijl, M. Westra, J. Kallenberg, C. G. M. |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Groningen Martini Hosp Universidade Federal de São Paulo (UNIFESP) Univ Wroclaw |
dc.contributor.author.fl_str_mv |
Souza, A. W. S. de [UNIFESP] Abdulahad, W. H. Sosicka, P. Bijzet, J. Limburg, P. C. Stegeman, C. A. Bijl, M. Westra, J. Kallenberg, C. G. M. |
dc.subject.eng.fl_str_mv |
anti-neutrophil cytoplasmic antibody-associated vasculitis glomerulonephritis HMGB1 monocyte chemoattractant protein-1 T cells |
topic |
anti-neutrophil cytoplasmic antibody-associated vasculitis glomerulonephritis HMGB1 monocyte chemoattractant protein-1 T cells |
description |
The objective of this study is to evaluate urinary high mobility group box 1 (HMGB1) levels as markers for active nephritis in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) in comparison with urinary CD4(+) effector memory T cells and urinary monocyte chemoattractant protein-1 (MCP-1). Twenty-four AAV patients with active nephritis and 12 healthy controls (HC) were evaluated. in nine patients, samples were also obtained during remission. Urinary levels of HMGB1 were measured by Western blot. CD4(+) T cells and CD4(+) effector memory T cells (CD4(+)CD45RO(+)CCR7(-)) were determined in urine and whole blood by flow cytometry. Measurement of urinary levels of MCP-1 and serum HMGB1 levels were performed by enzyme-linked immunosorbent assay (ELISA). AAV patients with active nephritis had higher median intensity of HMGB1 in urine than HC [10.3 (7.05-18.50) versus 5.8 (4.48-7.01); P = 0.004]. Both urinary HMGB1 and MCP-1 levels decreased significantly from active nephritis to remission. the urinary MCP-1/creatinine ratio correlated with Birmingham Vasculitis Activity Score (BVAS) (P = 0.042). No correlation was found between the HMGB1/creatinine ratio and 24-h proteinuria, estimated glomerular filtration rate (eGFR), MCP-1/creatinine ratio, BVAS and serum HMGB1. A positive correlation was found between urinary HMGB1/creatinine ratio and CD4(+) T cells/creatinine ratio (P = 0.028) and effector memory T cells/creatinine ratio (P = 0.039) in urine. Urinary HMGB1 levels are increased in AAV patients with active nephritis when compared with HC and patients in remission, and urinary HMGB1 levels are associated with CD4(+) T cells and CD4(+) effector memory T cells in urine. Measurement of urinary HMGB1 may be of additional value in identifying active glomerulonephritis in AAV patients. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:03Z |
dc.date.available.fl_str_mv |
2016-01-24T14:38:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinical and Experimental Immunology. Hoboken: Wiley-Blackwell, v. 178, n. 2, p. 270-278, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38364 http://dx.doi.org/10.1111/cei.12422 |
dc.identifier.issn.none.fl_str_mv |
0009-9104 |
dc.identifier.doi.none.fl_str_mv |
10.1111/cei.12422 |
dc.identifier.wos.none.fl_str_mv |
WOS:000342915800009 |
identifier_str_mv |
Clinical and Experimental Immunology. Hoboken: Wiley-Blackwell, v. 178, n. 2, p. 270-278, 2014. 0009-9104 10.1111/cei.12422 WOS:000342915800009 |
url |
http://repositorio.unifesp.br/handle/11600/38364 http://dx.doi.org/10.1111/cei.12422 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Clinical and Experimental Immunology |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
270-278 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764107289460736 |