Pretreatment with pentoxifylline attenuates lung injury induced by intestinal ischemia/reperfusion in rats

• Autor(a) principal: Marqui, Carlos Eduardo [UNIFESP]
• Data de Publicação: 2011
• Outros Autores: Silva, Helga Cristina Almeida da [UNIFESP], Ferez, David [UNIFESP], Cavassani, Sâmia Santos [UNIFESP], Moraes, Juliana Britto [UNIFESP], Silva, Danielle Aparecida Marino da [UNIFESP], Simões, Ricardo Santos [UNIFESP], Lopes, Caroline Aparecida [UNIFESP], Taha, Murched Omar [UNIFESP], Oliveira-Júnior, Itamar Souza [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-86502011000600006; http://repositorio.unifesp.br/handle/11600/6741
• topic: Lung Injury; Ischemia; Reperfusion Injury; Oxidative Stress; Pentoxifylline; Rats; Lesão Pulmonar; Isquemia; Traumatismo por Reperfusão; Estresse Oxidativo; Pentoxifilina; Ratos

PURPOSE: To investigate the protective effect of pentoxifylline against the lung injury observed after intestinal ischemia (I) followed by a period of reperfusion (R). METHODS: Twenty-eight male Wistar rats were equally divided into 4 experimental groups and operated under ketamine-xylazine anesthesia. (1) Sham: falsely-operated animals; (2) SS+IR: intestinal ischemia was accomplished by clipping the superior mesenteric artery during 60 minutes, with an administration of a standard volume of saline solution (SS) 5 min before the end of the ischemia period; the clip was then releases or a 120-min period of reperfusion; (3) I+PTX+R: ischemia as above, PTX was administered (25 mg/kg) and the gut reperfused as above; (4) PTX+I+PTX+R: Five minutes before arterial occlusion PTX was administered; the superior mesenteric artery was then clipped for 60 minutes. After 55-min ischemia, an additional dosis of PTX was administered; the clip was removed for reperfusion as above. At the 60th min of reperfusion a third dosis of PTX was administered. RESULTS: PTX markedly attenuated lung injury as manifested by significant decreases (all P<0.001 as compared with the SS+IR group) of pulmonary wet/dry tissue weight ratio, total protein content, myeloperoxidase activity and tumor necrosis factor-alpha. Moreover, it was apparent that in the group PTX+I+PTX+R the improvements have been even more significant. CONCLUSION: PTX exerted a protective effect on the lung from the injuries caused by intestinal ischemia/reperfusion.