Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34724 http://dx.doi.org/10.1016/j.procbio.2011.12.012 |
Resumo: | Shrimp head waste is a major byproduct of crustacean processing in North-eastern Brazil and represents an interesting source of bioactive molecules. Additionally, its use increases the sustainability of processing fishery products. the present study reports a process developed for recovering bioactive molecules from shrimp heads through autolysis. A protein hydrolysate (120 +/- 0.4 g) formed by a 9% (w/v) solution was recovered and lyophilized from I kg of shrimp heads. Approximately 195 +/- 0.5 mg of carotenoids was recovered as an ethanolic extract. the recovery of chitin and chitosan were 25 +/- 2 g kg(-1) and 17 +/- 4 g kg(-1) wet processing waste, respectively. Chitosans were characterized by C-13 NMR, and FT-IR analysis and exhibited a variable degree of deacetylation (60-80%). Sulfated glycosaminoglycans that exhibited electrophoretic migration similar to mammalian standards were also recovered (79 +/- 2 mg kg(-1) wet processing waste), and their degradation products suggested the presence of C6-sulfated heparan sulfate. These data point to the feasibility of an integrated process for isolating highly bioactive molecules, such as sulfated- and amino-polysaccharides, with a broad spectrum of applications from shrimp processing waste. (C) 2011 Elsevier B.V. All rights reserved. |
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Cahú, Thiago BarbosaSantos, Suzan DinizMendes, Aline [UNIFESP]Cóordula, Carolina Ribeiro [UNIFESP]Chavante, Suely Ferreira [UNIFESP]Carvalho, Luiz Bezerra deNader, Helena Bonciani [UNIFESP]Bezerra, Ranilson de SouzaUniversidade Federal de Pernambuco (UFPE)Universidade Federal de São Paulo (UNIFESP)Univ Fed Rio Grande do Norte2016-01-24T14:26:59Z2016-01-24T14:26:59Z2012-04-01Process Biochemistry. Oxford: Elsevier B.V., v. 47, n. 4, p. 570-577, 2012.1359-5113http://repositorio.unifesp.br/handle/11600/34724http://dx.doi.org/10.1016/j.procbio.2011.12.012WOS000302593800002.pdf10.1016/j.procbio.2011.12.012WOS:000302593800002Shrimp head waste is a major byproduct of crustacean processing in North-eastern Brazil and represents an interesting source of bioactive molecules. Additionally, its use increases the sustainability of processing fishery products. the present study reports a process developed for recovering bioactive molecules from shrimp heads through autolysis. A protein hydrolysate (120 +/- 0.4 g) formed by a 9% (w/v) solution was recovered and lyophilized from I kg of shrimp heads. Approximately 195 +/- 0.5 mg of carotenoids was recovered as an ethanolic extract. the recovery of chitin and chitosan were 25 +/- 2 g kg(-1) and 17 +/- 4 g kg(-1) wet processing waste, respectively. Chitosans were characterized by C-13 NMR, and FT-IR analysis and exhibited a variable degree of deacetylation (60-80%). Sulfated glycosaminoglycans that exhibited electrophoretic migration similar to mammalian standards were also recovered (79 +/- 2 mg kg(-1) wet processing waste), and their degradation products suggested the presence of C6-sulfated heparan sulfate. These data point to the feasibility of an integrated process for isolating highly bioactive molecules, such as sulfated- and amino-polysaccharides, with a broad spectrum of applications from shrimp processing waste. (C) 2011 Elsevier B.V. All rights reserved.MPAConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FINEPEMBRAPAUFPEFACEPEUniv Fed Pernambuco, Lab Imunopatol Keizo Asami, Dept Bioquim, Lab Enzimol LABENZ, BR-50670420 Recife, PE, BrazilUniversidade Federal de São Paulo, INFAR, Lab Biol Mol, BR-04044020 São Paulo, BrazilUniv Fed Rio Grande do Norte, Ctr Biociencias, Dept Bioquim, BR-59072000 Natal, RN, BrazilUniversidade Federal de São Paulo, INFAR, Lab Biol Mol, BR-04044020 São Paulo, BrazilWeb of Science570-577engElsevier B.V.Process Biochemistryhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessAutolysisBioactive moleculesLitopenaeus vannameiPolysaccharidesProcessing wasteRecovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing wasteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000302593800002.pdfapplication/pdf1097376${dspace.ui.url}/bitstream/11600/34724/1/WOS000302593800002.pdfb70543fcd7d9982060cfef590115366fMD51open accessTEXTWOS000302593800002.pdf.txtWOS000302593800002.pdf.txtExtracted texttext/plain43029${dspace.ui.url}/bitstream/11600/34724/2/WOS000302593800002.pdf.txt4ad5c997cbefc8a192ed88c6af6d1e68MD52open access11600/347242022-11-04 15:45:37.924open accessoai:repositorio.unifesp.br:11600/34724Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T18:45:37Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
title |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
spellingShingle |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste Cahú, Thiago Barbosa Autolysis Bioactive molecules Litopenaeus vannamei Polysaccharides Processing waste |
title_short |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
title_full |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
title_fullStr |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
title_full_unstemmed |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
title_sort |
Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste |
author |
Cahú, Thiago Barbosa |
author_facet |
Cahú, Thiago Barbosa Santos, Suzan Diniz Mendes, Aline [UNIFESP] Cóordula, Carolina Ribeiro [UNIFESP] Chavante, Suely Ferreira [UNIFESP] Carvalho, Luiz Bezerra de Nader, Helena Bonciani [UNIFESP] Bezerra, Ranilson de Souza |
author_role |
author |
author2 |
Santos, Suzan Diniz Mendes, Aline [UNIFESP] Cóordula, Carolina Ribeiro [UNIFESP] Chavante, Suely Ferreira [UNIFESP] Carvalho, Luiz Bezerra de Nader, Helena Bonciani [UNIFESP] Bezerra, Ranilson de Souza |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de Pernambuco (UFPE) Universidade Federal de São Paulo (UNIFESP) Univ Fed Rio Grande do Norte |
dc.contributor.author.fl_str_mv |
Cahú, Thiago Barbosa Santos, Suzan Diniz Mendes, Aline [UNIFESP] Cóordula, Carolina Ribeiro [UNIFESP] Chavante, Suely Ferreira [UNIFESP] Carvalho, Luiz Bezerra de Nader, Helena Bonciani [UNIFESP] Bezerra, Ranilson de Souza |
dc.subject.eng.fl_str_mv |
Autolysis Bioactive molecules Litopenaeus vannamei Polysaccharides Processing waste |
topic |
Autolysis Bioactive molecules Litopenaeus vannamei Polysaccharides Processing waste |
description |
Shrimp head waste is a major byproduct of crustacean processing in North-eastern Brazil and represents an interesting source of bioactive molecules. Additionally, its use increases the sustainability of processing fishery products. the present study reports a process developed for recovering bioactive molecules from shrimp heads through autolysis. A protein hydrolysate (120 +/- 0.4 g) formed by a 9% (w/v) solution was recovered and lyophilized from I kg of shrimp heads. Approximately 195 +/- 0.5 mg of carotenoids was recovered as an ethanolic extract. the recovery of chitin and chitosan were 25 +/- 2 g kg(-1) and 17 +/- 4 g kg(-1) wet processing waste, respectively. Chitosans were characterized by C-13 NMR, and FT-IR analysis and exhibited a variable degree of deacetylation (60-80%). Sulfated glycosaminoglycans that exhibited electrophoretic migration similar to mammalian standards were also recovered (79 +/- 2 mg kg(-1) wet processing waste), and their degradation products suggested the presence of C6-sulfated heparan sulfate. These data point to the feasibility of an integrated process for isolating highly bioactive molecules, such as sulfated- and amino-polysaccharides, with a broad spectrum of applications from shrimp processing waste. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-04-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:26:59Z |
dc.date.available.fl_str_mv |
2016-01-24T14:26:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Process Biochemistry. Oxford: Elsevier B.V., v. 47, n. 4, p. 570-577, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34724 http://dx.doi.org/10.1016/j.procbio.2011.12.012 |
dc.identifier.issn.none.fl_str_mv |
1359-5113 |
dc.identifier.file.none.fl_str_mv |
WOS000302593800002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.procbio.2011.12.012 |
dc.identifier.wos.none.fl_str_mv |
WOS:000302593800002 |
identifier_str_mv |
Process Biochemistry. Oxford: Elsevier B.V., v. 47, n. 4, p. 570-577, 2012. 1359-5113 WOS000302593800002.pdf 10.1016/j.procbio.2011.12.012 WOS:000302593800002 |
url |
http://repositorio.unifesp.br/handle/11600/34724 http://dx.doi.org/10.1016/j.procbio.2011.12.012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Process Biochemistry |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
dc.format.none.fl_str_mv |
570-577 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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