Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection

Detalhes bibliográficos
Autor(a) principal: Shulzhenko, Natalia [UNIFESP]
Data de Publicação: 2001
Outros Autores: Morgun, Andrey [UNIFESP], Franco, Marcello Fabiano de [UNIFESP], Souza, Marcia Marcelino de [UNIFESP], Almeida, Dirceu Rodrigues de [UNIFESP], Diniz, Rosiane Viana Zuza [UNIFESP], Carvalho, Antonio Carlos [UNIFESP], Pacheco-Silva, Alvaro [UNIFESP], Gerbase-Lima, Maria [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1590/S0100-879X2001000600013
https://repositorio.unifesp.br/handle/11600/1190
Resumo: The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection.
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spelling Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejectionRejectionCardiac allograftImmunologic monitoringmRNAGene activationInterferon-gammaThe purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection.Universidade Federal de São Paulo (UNIFESP) Departamento de PediatriaUniversidade Federal de São Paulo (UNIFESP) Departamento de PatologiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaUNIFESP, Depto. de PediatriaUNIFESP, Depto. de PatologiaUNIFESP, EPM, Depto. de MedicinaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Shulzhenko, Natalia [UNIFESP]Morgun, Andrey [UNIFESP]Franco, Marcello Fabiano de [UNIFESP]Souza, Marcia Marcelino de [UNIFESP]Almeida, Dirceu Rodrigues de [UNIFESP]Diniz, Rosiane Viana Zuza [UNIFESP]Carvalho, Antonio Carlos [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Gerbase-Lima, Maria [UNIFESP]2015-06-14T13:29:26Z2015-06-14T13:29:26Z2001-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion779-784application/pdfhttps://dx.doi.org/10.1590/S0100-879X2001000600013Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001.10.1590/S0100-879X2001000600013S0100-879X2001000600013.pdf0100-879XS0100-879X2001000600013https://repositorio.unifesp.br/handle/11600/1190WOS:000169443400013engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T22:46:42Zoai:repositorio.unifesp.br/:11600/1190Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T22:46:42Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
title Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
spellingShingle Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
Shulzhenko, Natalia [UNIFESP]
Rejection
Cardiac allograft
Immunologic monitoring
mRNA
Gene activation
Interferon-gamma
title_short Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
title_full Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
title_fullStr Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
title_full_unstemmed Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
title_sort Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
author Shulzhenko, Natalia [UNIFESP]
author_facet Shulzhenko, Natalia [UNIFESP]
Morgun, Andrey [UNIFESP]
Franco, Marcello Fabiano de [UNIFESP]
Souza, Marcia Marcelino de [UNIFESP]
Almeida, Dirceu Rodrigues de [UNIFESP]
Diniz, Rosiane Viana Zuza [UNIFESP]
Carvalho, Antonio Carlos [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Gerbase-Lima, Maria [UNIFESP]
author_role author
author2 Morgun, Andrey [UNIFESP]
Franco, Marcello Fabiano de [UNIFESP]
Souza, Marcia Marcelino de [UNIFESP]
Almeida, Dirceu Rodrigues de [UNIFESP]
Diniz, Rosiane Viana Zuza [UNIFESP]
Carvalho, Antonio Carlos [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Gerbase-Lima, Maria [UNIFESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Shulzhenko, Natalia [UNIFESP]
Morgun, Andrey [UNIFESP]
Franco, Marcello Fabiano de [UNIFESP]
Souza, Marcia Marcelino de [UNIFESP]
Almeida, Dirceu Rodrigues de [UNIFESP]
Diniz, Rosiane Viana Zuza [UNIFESP]
Carvalho, Antonio Carlos [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Gerbase-Lima, Maria [UNIFESP]
dc.subject.por.fl_str_mv Rejection
Cardiac allograft
Immunologic monitoring
mRNA
Gene activation
Interferon-gamma
topic Rejection
Cardiac allograft
Immunologic monitoring
mRNA
Gene activation
Interferon-gamma
description The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection.
publishDate 2001
dc.date.none.fl_str_mv 2001-06-01
2015-06-14T13:29:26Z
2015-06-14T13:29:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1590/S0100-879X2001000600013
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001.
10.1590/S0100-879X2001000600013
S0100-879X2001000600013.pdf
0100-879X
S0100-879X2001000600013
https://repositorio.unifesp.br/handle/11600/1190
WOS:000169443400013
url https://dx.doi.org/10.1590/S0100-879X2001000600013
https://repositorio.unifesp.br/handle/11600/1190
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001.
10.1590/S0100-879X2001000600013
S0100-879X2001000600013.pdf
0100-879X
S0100-879X2001000600013
WOS:000169443400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 779-784
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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