Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1590/S0100-879X2001000600013 https://repositorio.unifesp.br/handle/11600/1190 |
Resumo: | The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection. |
id |
UFSP_f8b15707d36cc2a8d5e42b92cd9b91bb |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/1190 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejectionRejectionCardiac allograftImmunologic monitoringmRNAGene activationInterferon-gammaThe purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection.Universidade Federal de São Paulo (UNIFESP) Departamento de PediatriaUniversidade Federal de São Paulo (UNIFESP) Departamento de PatologiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaUNIFESP, Depto. de PediatriaUNIFESP, Depto. de PatologiaUNIFESP, EPM, Depto. de MedicinaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Shulzhenko, Natalia [UNIFESP]Morgun, Andrey [UNIFESP]Franco, Marcello Fabiano de [UNIFESP]Souza, Marcia Marcelino de [UNIFESP]Almeida, Dirceu Rodrigues de [UNIFESP]Diniz, Rosiane Viana Zuza [UNIFESP]Carvalho, Antonio Carlos [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Gerbase-Lima, Maria [UNIFESP]2015-06-14T13:29:26Z2015-06-14T13:29:26Z2001-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion779-784application/pdfhttps://dx.doi.org/10.1590/S0100-879X2001000600013Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001.10.1590/S0100-879X2001000600013S0100-879X2001000600013.pdf0100-879XS0100-879X2001000600013https://repositorio.unifesp.br/handle/11600/1190WOS:000169443400013engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T22:46:42Zoai:repositorio.unifesp.br/:11600/1190Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T22:46:42Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
title |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
spellingShingle |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection Shulzhenko, Natalia [UNIFESP] Rejection Cardiac allograft Immunologic monitoring mRNA Gene activation Interferon-gamma |
title_short |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
title_full |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
title_fullStr |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
title_full_unstemmed |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
title_sort |
Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection |
author |
Shulzhenko, Natalia [UNIFESP] |
author_facet |
Shulzhenko, Natalia [UNIFESP] Morgun, Andrey [UNIFESP] Franco, Marcello Fabiano de [UNIFESP] Souza, Marcia Marcelino de [UNIFESP] Almeida, Dirceu Rodrigues de [UNIFESP] Diniz, Rosiane Viana Zuza [UNIFESP] Carvalho, Antonio Carlos [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Gerbase-Lima, Maria [UNIFESP] |
author_role |
author |
author2 |
Morgun, Andrey [UNIFESP] Franco, Marcello Fabiano de [UNIFESP] Souza, Marcia Marcelino de [UNIFESP] Almeida, Dirceu Rodrigues de [UNIFESP] Diniz, Rosiane Viana Zuza [UNIFESP] Carvalho, Antonio Carlos [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Gerbase-Lima, Maria [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Shulzhenko, Natalia [UNIFESP] Morgun, Andrey [UNIFESP] Franco, Marcello Fabiano de [UNIFESP] Souza, Marcia Marcelino de [UNIFESP] Almeida, Dirceu Rodrigues de [UNIFESP] Diniz, Rosiane Viana Zuza [UNIFESP] Carvalho, Antonio Carlos [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Gerbase-Lima, Maria [UNIFESP] |
dc.subject.por.fl_str_mv |
Rejection Cardiac allograft Immunologic monitoring mRNA Gene activation Interferon-gamma |
topic |
Rejection Cardiac allograft Immunologic monitoring mRNA Gene activation Interferon-gamma |
description |
The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and without rejection were 0.116 and zero for CD40L (P<0.003), 0.080 and zero for IFN-gamma (P<0.0009), and 0.156 and zero for FasL (P<0.002), respectively. In addition, the levels of IFN-gamma mRNA were significantly increased 7 to 15 days before the appearance of histological evidence of rejection (median of 0.086 in pre-rejection biopsies), i.e., they presented a predictive value. This study provides further evidence of heightened expression of immune activation genes during rejection and shows that some of these markers may present predictive value for the occurrence of acute rejection. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-06-01 2015-06-14T13:29:26Z 2015-06-14T13:29:26Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1590/S0100-879X2001000600013 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001. 10.1590/S0100-879X2001000600013 S0100-879X2001000600013.pdf 0100-879X S0100-879X2001000600013 https://repositorio.unifesp.br/handle/11600/1190 WOS:000169443400013 |
url |
https://dx.doi.org/10.1590/S0100-879X2001000600013 https://repositorio.unifesp.br/handle/11600/1190 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 779-784, 2001. 10.1590/S0100-879X2001000600013 S0100-879X2001000600013.pdf 0100-879X S0100-879X2001000600013 WOS:000169443400013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
779-784 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268426815799296 |