Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest

Detalhes bibliográficos
Autor(a) principal: Novello, Mariana
Data de Publicação: 2013
Outros Autores: Rodrigues, Jucelene Fernandes, Pinheiro, Fábio, Oliveira, Giancarlo Conde Xavier, Veasey, Elizabeth Ann, Koehler, Samantha [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.4238/2013.September.3.3
http://repositorio.unifesp.br/handle/11600/35753
Resumo: Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petropolis population. the mean number of alleles per locus was 8.8 between populations. the mean values of the observed and expected heterozygosities were 0.541 (ranging from 0 to 1) and 0.639 (ranging from 0 to 0.9), respectively. Cross-amplifications were performed in 7 additional Epidendroideae species, and at least 2 loci were successful in 3 additional Cattleya species, Epidendrum secundum, and Brasiliorchis gracilis. All markers described herein will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of C. coccinea and related species.
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spelling Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic ForestCattleyaEpidendroideaeLaeliinaeMicrosatellite markersOrchidaceaeSophronitisMicrosatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petropolis population. the mean number of alleles per locus was 8.8 between populations. the mean values of the observed and expected heterozygosities were 0.541 (ranging from 0 to 1) and 0.639 (ranging from 0 to 0.9), respectively. Cross-amplifications were performed in 7 additional Epidendroideae species, and at least 2 loci were successful in 3 additional Cattleya species, Epidendrum secundum, and Brasiliorchis gracilis. All markers described herein will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of C. coccinea and related species.Univ São Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Piracicaba, SP, BrazilInst Bot São Paulo, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2006/55121-3FAPESP: 2011/18532-3Funpec-editoraUniversidade de São Paulo (USP)Inst Bot São PauloUniversidade Federal de São Paulo (UNIFESP)Novello, MarianaRodrigues, Jucelene FernandesPinheiro, FábioOliveira, Giancarlo Conde XavierVeasey, Elizabeth AnnKoehler, Samantha [UNIFESP]2016-01-24T14:30:58Z2016-01-24T14:30:58Z2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion3274-3278http://dx.doi.org/10.4238/2013.September.3.3Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 12, n. 3, p. 3274-3278, 2013.10.4238/2013.September.3.31676-5680http://repositorio.unifesp.br/handle/11600/35753WOS:000331717400117engGenetics and Molecular Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:30:58Zoai:repositorio.unifesp.br/:11600/35753Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:30:58Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
title Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
spellingShingle Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
Novello, Mariana
Cattleya
Epidendroideae
Laeliinae
Microsatellite markers
Orchidaceae
Sophronitis
title_short Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
title_full Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
title_fullStr Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
title_full_unstemmed Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
title_sort Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
author Novello, Mariana
author_facet Novello, Mariana
Rodrigues, Jucelene Fernandes
Pinheiro, Fábio
Oliveira, Giancarlo Conde Xavier
Veasey, Elizabeth Ann
Koehler, Samantha [UNIFESP]
author_role author
author2 Rodrigues, Jucelene Fernandes
Pinheiro, Fábio
Oliveira, Giancarlo Conde Xavier
Veasey, Elizabeth Ann
Koehler, Samantha [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Inst Bot São Paulo
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Novello, Mariana
Rodrigues, Jucelene Fernandes
Pinheiro, Fábio
Oliveira, Giancarlo Conde Xavier
Veasey, Elizabeth Ann
Koehler, Samantha [UNIFESP]
dc.subject.por.fl_str_mv Cattleya
Epidendroideae
Laeliinae
Microsatellite markers
Orchidaceae
Sophronitis
topic Cattleya
Epidendroideae
Laeliinae
Microsatellite markers
Orchidaceae
Sophronitis
description Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petropolis population. the mean number of alleles per locus was 8.8 between populations. the mean values of the observed and expected heterozygosities were 0.541 (ranging from 0 to 1) and 0.639 (ranging from 0 to 0.9), respectively. Cross-amplifications were performed in 7 additional Epidendroideae species, and at least 2 loci were successful in 3 additional Cattleya species, Epidendrum secundum, and Brasiliorchis gracilis. All markers described herein will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of C. coccinea and related species.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2016-01-24T14:30:58Z
2016-01-24T14:30:58Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/2013.September.3.3
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 12, n. 3, p. 3274-3278, 2013.
10.4238/2013.September.3.3
1676-5680
http://repositorio.unifesp.br/handle/11600/35753
WOS:000331717400117
url http://dx.doi.org/10.4238/2013.September.3.3
http://repositorio.unifesp.br/handle/11600/35753
identifier_str_mv Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 12, n. 3, p. 3274-3278, 2013.
10.4238/2013.September.3.3
1676-5680
WOS:000331717400117
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3274-3278
dc.publisher.none.fl_str_mv Funpec-editora
publisher.none.fl_str_mv Funpec-editora
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268380309356544

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