Analysis of Genomic Regions of Trichoderma harzianum IOC-3844 Related to Biomass Degradation

• Autor(a) principal: Crucello, Aline
• Data de Publicação: 2015
• Outros Autores: Sforca, Danilo Augusto, Crivelente Horta, Maria Augusta, Santos, Clelton Aparecido dos, Carvalho Viana, Americo Jose, Beloti, Lilian Luzia, Szymanski de Toledo, Marcelo Augusto, Vincentz, Michel, Kuroshu, Reginaldo Massanobu [UNIFESP], Souza, Anete Pereira de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1371/journal.pone.0122122; http://repositorio.unifesp.br/handle/11600/38990

Trichoderma harzianum IOC-3844 secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications in second-generation bioethanol production. However, the T. harzianum biomass degradation mechanism has not been well explored at the genetic level. the present work investigates six genomic regions (similar to 150 kbp each) in this fungus that are enriched with genes related to biomass conversion. A BAC library consisting of 5,760 clones was constructed, with an average insert length of 90 kbp. the assembled BAC sequences revealed 232 predicted genes, 31.5% of which were related to catabolic pathways, including those involved in biomass degradation. An expression profile analysis based on RNA-Seq data demonstrated that putative regulatory elements, such as membrane transport proteins and transcription factors, are located in the same genomic regions as genes related to carbohydrate metabolism and exhibit similar expression profiles. Thus, we demonstrate a rapid and efficient tool that focuses on specific genomic regions by combining a BAC library with transcriptomic data. This is the first BAC-based structural genomic study of the cellulolytic fungus T. harzianum, and its findings provide new perspectives regarding the use of this species in biomass degradation processes.