Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome

Detalhes bibliográficos
Autor(a) principal: Prata Lima, M.f.
Data de Publicação: 2004
Outros Autores: Baracat, Edmund Chada [UNIFESP], Simões, Manuel de Jesus [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000016vb4
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2004000700007
http://repositorio.unifesp.br/handle/11600/2155
Resumo: The current study was conducted to investigate the relationship between melatonin and chronic anovulation. Adult (3-4 months old) female Wistar rats were submitted to pinealectomy: group I: pinealectomized ovariectomized melatonin-treated (N = 10); group II: pinealectomized ovariectomized placebo-treated (N = 12); group III: pinealectomized light-treated placebo-treated(N = 10) or maintained under continuous light; group IV: maintained under continuous light, ovariectomized melatonin-treated (N = 22); group V: maintained under continuous light, ovariectomized placebo-treated (N = 10); group VI: maintained under continuous light placebo-treated (N = 10). In order to assess ovarian modifications, unilateral ovariectomy was performed during the fourth month in groups I, II, IV, V and the other ovary was removed after 8 months. Ovariectomy was performed in groups III and VI only after eight months. Melatonin (200 µg/100 g body weight) dissolved in 0.02 ml absolute ethanol was injected intramuscularly daily during the last 4 months into groups I and IV. The other groups were treated with placebo (NaCl). The ovarian cysts were analyzed and their area, perimeter and maximum diameter, as well as the thickness of the ovarian capsule were measured. Daily colpocytological smears were performed throughout the study. Persistent estrous condition and ovarian cysts were observed in all groups. In pinealectomized rats the ovarian and vaginal alterations disappeared at the end of the study and in rats maintained under continuous light the vaginal and ovarian polycystic aspect was reversed only in those treated with melatonin. We conclude that melatonin may act on the ovarian response reverting chronic anovulation induced by pinealectomy or continuous light.
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spelling Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndromeMelatoninPolycystic ovary syndromePineal bodyAnovulationThe current study was conducted to investigate the relationship between melatonin and chronic anovulation. Adult (3-4 months old) female Wistar rats were submitted to pinealectomy: group I: pinealectomized ovariectomized melatonin-treated (N = 10); group II: pinealectomized ovariectomized placebo-treated (N = 12); group III: pinealectomized light-treated placebo-treated(N = 10) or maintained under continuous light; group IV: maintained under continuous light, ovariectomized melatonin-treated (N = 22); group V: maintained under continuous light, ovariectomized placebo-treated (N = 10); group VI: maintained under continuous light placebo-treated (N = 10). In order to assess ovarian modifications, unilateral ovariectomy was performed during the fourth month in groups I, II, IV, V and the other ovary was removed after 8 months. Ovariectomy was performed in groups III and VI only after eight months. Melatonin (200 µg/100 g body weight) dissolved in 0.02 ml absolute ethanol was injected intramuscularly daily during the last 4 months into groups I and IV. The other groups were treated with placebo (NaCl). The ovarian cysts were analyzed and their area, perimeter and maximum diameter, as well as the thickness of the ovarian capsule were measured. Daily colpocytological smears were performed throughout the study. Persistent estrous condition and ovarian cysts were observed in all groups. In pinealectomized rats the ovarian and vaginal alterations disappeared at the end of the study and in rats maintained under continuous light the vaginal and ovarian polycystic aspect was reversed only in those treated with melatonin. We conclude that melatonin may act on the ovarian response reverting chronic anovulation induced by pinealectomy or continuous light.Faculdade de Medicina do Triângulo Mineiro Disciplina de Ginecologia e ObstetríciaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de GinecologiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de HistologiaUNIFESP, EPM, Depto. de GinecologiaUNIFESP, EPM, Depto. de HistologiaSciELOAssociação Brasileira de Divulgação CientíficaFaculdade de Medicina do Triângulo Mineiro Disciplina de Ginecologia e ObstetríciaUniversidade Federal de São Paulo (UNIFESP)Prata Lima, M.f.Baracat, Edmund Chada [UNIFESP]Simões, Manuel de Jesus [UNIFESP]2015-06-14T13:31:11Z2015-06-14T13:31:11Z2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion987-995application/pdfhttp://dx.doi.org/10.1590/S0100-879X2004000700007Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 987-995, 2004.10.1590/S0100-879X2004000700007S0100-879X2004000700007.pdf0100-879XS0100-879X2004000700007http://repositorio.unifesp.br/handle/11600/2155WOS:000222450200007ark:/48912/0013000016vb4engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:26:28Zoai:repositorio.unifesp.br/:11600/2155Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T21:00:22.661115Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
title Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
spellingShingle Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
Prata Lima, M.f.
Melatonin
Polycystic ovary syndrome
Pineal body
Anovulation
title_short Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
title_full Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
title_fullStr Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
title_full_unstemmed Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
title_sort Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: similarity with polycystic ovary syndrome
author Prata Lima, M.f.
author_facet Prata Lima, M.f.
Baracat, Edmund Chada [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
author_role author
author2 Baracat, Edmund Chada [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Faculdade de Medicina do Triângulo Mineiro Disciplina de Ginecologia e Obstetrícia
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Prata Lima, M.f.
Baracat, Edmund Chada [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
dc.subject.por.fl_str_mv Melatonin
Polycystic ovary syndrome
Pineal body
Anovulation
topic Melatonin
Polycystic ovary syndrome
Pineal body
Anovulation
description The current study was conducted to investigate the relationship between melatonin and chronic anovulation. Adult (3-4 months old) female Wistar rats were submitted to pinealectomy: group I: pinealectomized ovariectomized melatonin-treated (N = 10); group II: pinealectomized ovariectomized placebo-treated (N = 12); group III: pinealectomized light-treated placebo-treated(N = 10) or maintained under continuous light; group IV: maintained under continuous light, ovariectomized melatonin-treated (N = 22); group V: maintained under continuous light, ovariectomized placebo-treated (N = 10); group VI: maintained under continuous light placebo-treated (N = 10). In order to assess ovarian modifications, unilateral ovariectomy was performed during the fourth month in groups I, II, IV, V and the other ovary was removed after 8 months. Ovariectomy was performed in groups III and VI only after eight months. Melatonin (200 µg/100 g body weight) dissolved in 0.02 ml absolute ethanol was injected intramuscularly daily during the last 4 months into groups I and IV. The other groups were treated with placebo (NaCl). The ovarian cysts were analyzed and their area, perimeter and maximum diameter, as well as the thickness of the ovarian capsule were measured. Daily colpocytological smears were performed throughout the study. Persistent estrous condition and ovarian cysts were observed in all groups. In pinealectomized rats the ovarian and vaginal alterations disappeared at the end of the study and in rats maintained under continuous light the vaginal and ovarian polycystic aspect was reversed only in those treated with melatonin. We conclude that melatonin may act on the ovarian response reverting chronic anovulation induced by pinealectomy or continuous light.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
2015-06-14T13:31:11Z
2015-06-14T13:31:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2004000700007
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 987-995, 2004.
10.1590/S0100-879X2004000700007
S0100-879X2004000700007.pdf
0100-879X
S0100-879X2004000700007
http://repositorio.unifesp.br/handle/11600/2155
WOS:000222450200007
dc.identifier.dark.fl_str_mv ark:/48912/0013000016vb4
url http://dx.doi.org/10.1590/S0100-879X2004000700007
http://repositorio.unifesp.br/handle/11600/2155
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 987-995, 2004.
10.1590/S0100-879X2004000700007
S0100-879X2004000700007.pdf
0100-879X
S0100-879X2004000700007
WOS:000222450200007
ark:/48912/0013000016vb4
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 987-995
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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