ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica
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| Data de Publicação: | 2016 |
| Tipo de documento: | Dissertação |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFTM |
| Texto Completo: | http://bdtd.uftm.edu.br/handle/tede/551 |
Resumo: | A distribuição topográfica dos neurônios eferentes vagais vem sendo estudada principalmente pelo uso de traçadores retrógrados aplicados aos segmentos específicos de órgãos ou parte proximal de nervos seccionados. O objetivo deste trabalho foi avaliar se a expressão induzida de marcadores de lesão pode ser usada no estudo da distribuição e código químico no núcleo dorsal motor do nervo vago (DMV). Para este fim, ratos foram submetidos à secção de um dos três ramos do vago subdiafragmático anterior. Cinco dias após a axotomia os ratos foram perfundidos e reações de imunofluorescência realizadas para os marcadores de lesão: fator 3 ativador da transcrição (ATF-3), nestina e proteína 43 associada ao crescimento (GAP-43), bem como para marcadores neuronais: colina acetiltransferase (ChAT), tirosina hidroxilase (TH), óxido nítrico sintase neuronal (nNOS). A vagotomia periférica resultou em um rápido aumento na expressão de ATF-3, mas não de nestina ou GAP-43. Usando o traçador retrógrado toxina colérica subunidade foi estimado que 95,2± 0,7% dos neurônios do DMV respondem à vagotomia com um aumento na marcação anti-ATF-3. O número de neurônios que projetam pelos ramos gástrico anterior, hepático e acessório celíaco alcança um máximo nos níveis 33, 3 e 121 m, respectivamente. O ramo gástrico anterior está representado medialmente, o acessório celíaco lateralmente e o ramo hepático homogeneamente no DMV. A marcação anti-TH ocorreu exclusivamente em neurônios que projetam pelo ramo gástrico anterior. Foi observado um aumento significativo na porcentagem de neurônios nNOS positivos após axotomia. Dessa forma, a expressão induzida do ATF-3 é uma ferramenta útil na análise da distribuição de neurônios autonômicos não mielinizados. |
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ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periféricaNúcleo dorsal motor do nervo vago.ATF-3.Axotomia.Dorsal motor nucleus of vagus nerve.ATF-3.Axotomy.Ciências BiológicasA distribuição topográfica dos neurônios eferentes vagais vem sendo estudada principalmente pelo uso de traçadores retrógrados aplicados aos segmentos específicos de órgãos ou parte proximal de nervos seccionados. O objetivo deste trabalho foi avaliar se a expressão induzida de marcadores de lesão pode ser usada no estudo da distribuição e código químico no núcleo dorsal motor do nervo vago (DMV). Para este fim, ratos foram submetidos à secção de um dos três ramos do vago subdiafragmático anterior. Cinco dias após a axotomia os ratos foram perfundidos e reações de imunofluorescência realizadas para os marcadores de lesão: fator 3 ativador da transcrição (ATF-3), nestina e proteína 43 associada ao crescimento (GAP-43), bem como para marcadores neuronais: colina acetiltransferase (ChAT), tirosina hidroxilase (TH), óxido nítrico sintase neuronal (nNOS). A vagotomia periférica resultou em um rápido aumento na expressão de ATF-3, mas não de nestina ou GAP-43. Usando o traçador retrógrado toxina colérica subunidade foi estimado que 95,2± 0,7% dos neurônios do DMV respondem à vagotomia com um aumento na marcação anti-ATF-3. O número de neurônios que projetam pelos ramos gástrico anterior, hepático e acessório celíaco alcança um máximo nos níveis 33, 3 e 121 m, respectivamente. O ramo gástrico anterior está representado medialmente, o acessório celíaco lateralmente e o ramo hepático homogeneamente no DMV. A marcação anti-TH ocorreu exclusivamente em neurônios que projetam pelo ramo gástrico anterior. Foi observado um aumento significativo na porcentagem de neurônios nNOS positivos após axotomia. Dessa forma, a expressão induzida do ATF-3 é uma ferramenta útil na análise da distribuição de neurônios autonômicos não mielinizados.The topographical distribution of vagal efferent neurons has been studied mainly by the use of retrograde tracers applied to specific segments of organs or proximal part of severed nerves. The aim of this study was to evaluate whether the induced expression of lesion markers can be used to study the distribution and the chemical code of dorsal motor nucleus of vagus nerve (DMV). To this end, rats were submitted to section of one of the three branches of the anterior subdiaphragmatic vagus. Five days after axotomy, rats were perfused and immunofluorescence reactions performed for lesions markers: activating transcriptional factor 3 (ATF-3), nestin and Growth associated proteins 43 (GAP-43), as well as for neuronal markers: choline acetyltransferase (ChAT), tyrosine hydroxylase (TH) and neuronal nitric oxide synthase (nNOS). Peripheral vagotomy resulted in a rapid increase in ATF-3 expression but not for nestin or GAP-43. Using the retrograde tracer choleric toxin subunit it was estimated that 95.2 ± 0.7% of DMV neurons respond to vagotomy with an increase in antiATF-3 labeling. The anterior gastric, hepatic and accessory celiac branch neurons reached a maximum number at the level 33, 3 and 121 m, respectively. The anterior gastric branch is represented medially, the accessory celiac laterally and the hepatic branch homogeneously at DMV. The anti-TH labeling occurred exclusively in anterior gastric projecting neurons. It was observed a significant increase in the percentage of positive nNOS neurons after axotomy. Therefore, the induced expression of ATF-3 is a useful tool for analysis of distribution of unmyelinated autonomic neurons.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal do Triângulo MineiroInstituto de Ciências da Saúde - ICS::Curso de MedicinaBrasilUFTMPrograma de Pós-Graduação em Ciências FisiológicasRODRIGUES, Aldo Rogelis Aquiles83546448634http://lattes.cnpq.br/6645292290768657TAVARES, Eduardo Henrique2018-05-02T20:25:07Z2016-11-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfTAVARES, Eduardo Henrique. ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica. 2016. 58f. Dissertação (Mestrado em Ciências Fisiológicas) - Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Triângulo Mineiro, Uberaba, 2016.http://bdtd.uftm.edu.br/handle/tede/551porAbankwa D, Küry P, Müller HW. Dynamic changes in gene expression profiles following axotomy of projection fibres in the Mammalian CNS. Mol Cell Neurosci 21(3):421-435, 2002. Abe N, Cavalli V. Nerve injury signaling. Curr Opin Neurobiol 18(3):276-83, 2008. Aldskogius H, Barron KD, Regal R. Axon reaction in dorsal motor vagal and hypoglossal neurons of the adult rat. Light microscopy and RNA-cytochemistry. J Comp Neurol 193(1):165-177, 1980. Aldskogius H, Barron KD, Regal R. Axon reaction in hypoglossal and dorsal motor vagal neurons of adult rat: incorporation of [3H]leucine. Exp Neurol 85(1):139-151, 1984. Armstrong DM, Ross CA, Pickel VM, Joh TH, Reis DJ. 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World J Gastroenterol 12(20): 3271–3274, 2006http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFTMinstname:Universidade Federal do Triangulo Mineiro (UFTM)instacron:UFTM2019-06-26T19:14:43Zoai:bdtd.uftm.edu.br:tede/551Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.uftm.edu.br/PUBhttp://bdtd.uftm.edu.br/oai/requestbdtd@uftm.edu.br||bdtd@uftm.edu.bropendoar:2019-06-26T19:14:43Biblioteca Digital de Teses e Dissertações da UFTM - Universidade Federal do Triangulo Mineiro (UFTM)false |
| dc.title.none.fl_str_mv |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| title |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| spellingShingle |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica TAVARES, Eduardo Henrique Núcleo dorsal motor do nervo vago. ATF-3. Axotomia. Dorsal motor nucleus of vagus nerve. ATF-3. Axotomy. Ciências Biológicas |
| title_short |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| title_full |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| title_fullStr |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| title_full_unstemmed |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| title_sort |
ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica |
| author |
TAVARES, Eduardo Henrique |
| author_facet |
TAVARES, Eduardo Henrique |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
RODRIGUES, Aldo Rogelis Aquiles 83546448634 http://lattes.cnpq.br/6645292290768657 |
| dc.contributor.author.fl_str_mv |
TAVARES, Eduardo Henrique |
| dc.subject.por.fl_str_mv |
Núcleo dorsal motor do nervo vago. ATF-3. Axotomia. Dorsal motor nucleus of vagus nerve. ATF-3. Axotomy. Ciências Biológicas |
| topic |
Núcleo dorsal motor do nervo vago. ATF-3. Axotomia. Dorsal motor nucleus of vagus nerve. ATF-3. Axotomy. Ciências Biológicas |
| description |
A distribuição topográfica dos neurônios eferentes vagais vem sendo estudada principalmente pelo uso de traçadores retrógrados aplicados aos segmentos específicos de órgãos ou parte proximal de nervos seccionados. O objetivo deste trabalho foi avaliar se a expressão induzida de marcadores de lesão pode ser usada no estudo da distribuição e código químico no núcleo dorsal motor do nervo vago (DMV). Para este fim, ratos foram submetidos à secção de um dos três ramos do vago subdiafragmático anterior. Cinco dias após a axotomia os ratos foram perfundidos e reações de imunofluorescência realizadas para os marcadores de lesão: fator 3 ativador da transcrição (ATF-3), nestina e proteína 43 associada ao crescimento (GAP-43), bem como para marcadores neuronais: colina acetiltransferase (ChAT), tirosina hidroxilase (TH), óxido nítrico sintase neuronal (nNOS). A vagotomia periférica resultou em um rápido aumento na expressão de ATF-3, mas não de nestina ou GAP-43. Usando o traçador retrógrado toxina colérica subunidade foi estimado que 95,2± 0,7% dos neurônios do DMV respondem à vagotomia com um aumento na marcação anti-ATF-3. O número de neurônios que projetam pelos ramos gástrico anterior, hepático e acessório celíaco alcança um máximo nos níveis 33, 3 e 121 m, respectivamente. O ramo gástrico anterior está representado medialmente, o acessório celíaco lateralmente e o ramo hepático homogeneamente no DMV. A marcação anti-TH ocorreu exclusivamente em neurônios que projetam pelo ramo gástrico anterior. Foi observado um aumento significativo na porcentagem de neurônios nNOS positivos após axotomia. Dessa forma, a expressão induzida do ATF-3 é uma ferramenta útil na análise da distribuição de neurônios autonômicos não mielinizados. |
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2016 |
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2016-11-21 2018-05-02T20:25:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
TAVARES, Eduardo Henrique. ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica. 2016. 58f. Dissertação (Mestrado em Ciências Fisiológicas) - Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Triângulo Mineiro, Uberaba, 2016. http://bdtd.uftm.edu.br/handle/tede/551 |
| identifier_str_mv |
TAVARES, Eduardo Henrique. ATF-3 como marcador de lesão e de padrões topográficos em neurônios autonômicos do núcleo dorsal motor do nervo vago após axotomia periférica. 2016. 58f. Dissertação (Mestrado em Ciências Fisiológicas) - Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Triângulo Mineiro, Uberaba, 2016. |
| url |
http://bdtd.uftm.edu.br/handle/tede/551 |
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por |
| language |
por |
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Universidade Federal do Triângulo Mineiro Instituto de Ciências da Saúde - ICS::Curso de Medicina Brasil UFTM Programa de Pós-Graduação em Ciências Fisiológicas |
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Universidade Federal do Triângulo Mineiro Instituto de Ciências da Saúde - ICS::Curso de Medicina Brasil UFTM Programa de Pós-Graduação em Ciências Fisiológicas |
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Biblioteca Digital de Teses e Dissertações da UFTM - Universidade Federal do Triangulo Mineiro (UFTM) |
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