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Atividade da acetilcolinesterase em populações sazonais experimentais de Zaprionus indianus Gupta 1970 (Diptera: Drosophilidae) expostas ao organofosforado MalathionAcetilcolinesterase.Organofosforado.Sazonalidade.Acetylcholinesterase.Organophosphate.Seasonality.Ciências AmbientaisA espécie Zaprionus indianus é uma espécie originaria da Africa porém é encontrada em muitos continentes do planeta, incluindo o continente americano. Essa espécie possui um potencial de se tornar praga em culturas de frutas que são amplamente distribuidas no Brasil, e o seu controle bem como de outros grupos de insetos, incluindo vetores de doenças como o Aedes aegypti, é realizado atraves da utilização de inseticidas organofosforados, em áreas rurais e urbanas. O presente trabalho teve como objetivo avaliar o potencial bioindicador de contaminação por Malathion da enzima Acetilcolinesterase, alvo do inseticida, em gerações F1 de populações de Uberaba e Peirópolis da espécie Zaprionus Indianus. Além de verificar se existe diferença significativa relacionada ao sexo, bem como o comportamento sazonal, relacionado a atividade catalitica dessa enzima em estações secas e úmidas. Foram realizadas análises de espectofotometria de luz e eletroforetica em gel de poliacrilamida para avaliar a ação e inibição enzimática, utilizando as cabeças dos individuos experimentais. Os dados encontraram valores significativos em comparações de atividades encontradas em populações de estações diferentes, além de uma correspondencia entre o final de uma estação e meio de outra. Os testes de inibição demostração valores inferiores de atividade enzimatica quando exposta ao inseticida Malathion em populações das estações de março e junho. Valores não significativos foram encontrados para comparações entre machos e fêmeas. De acordo com os resultados das análises, a enzima AChE mostrou um alto potencial como bioindicadora de contaminação por Malathion.Zaprionus indianus is a species originally from Africa but is found on many continents of the planet, including the American continent. This species has the potential to become a pest in fruit crops that are widely distributed in Brazil, and its control as well as other insect groups, including disease vectors such as Aedes aegypti, is accomplished through the use of organophosphate insecticides, in rural and urban areas. The present work aimed to evaluate the potential bioindicator of Malathion contamination of the enzyme Acetylcholinesterase, target of the insecticide, in F1 generations of Uberaba and Peirópolis populations of Zaprionus Indianus. In addition to verifying if there is significant difference related to sex, as well as seasonal behavior, related to the catalytic activity of this enzyme in dry and humid seasons. Light spectrophotometry and polyacrylamide gel electrophoretic analysis were performed to evaluate the enzymatic action and inhibition using the heads of the experimental subjects. The data found significant values in comparisons of activities found in populations of different seasons, as well as a correspondence between the end of one season and a half of another. Inhibition tests showed lower values of enzyme activity when exposed to the Malathion insecticide in populations of the March and June seasons. Non-significant values were found for comparisons between males and females. According to the results of the analyzes, the enzyme AChE showed a high potential as a bioindicator of Malathion contamination.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal do Triângulo MineiroInstituto de Ciências Biológicas e Naturais - ICBNBrasilUFTMPrograma de Pós-Graduação Interdisciplinar em Biociências AplicadasGALEGO, Luís Gustavo da Conceição90909860653http://lattes.cnpq.br/9101105344317706MENDONÇA, Ricardo José99594242604http://lattes.cnpq.br/5588755574505816NUNES, Polyana Ferreira2019-12-02T13:09:32Z2019-09-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfNUNES, Polyana Ferreira. Atividade da acetilcolinesterase em populações sazonais experimentais de Zaprionus indianus Gupta 1970 (Diptera: Drosophilidae) expostas ao organofosforado Malathion. 2019. 53f. 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Determinação de proteínas totais via espectrofotometria: vantagens e desvantagens dos métodos existentes. Química Nova, São Paulo, v. 21, n. 6, p. 787-793, 1998.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFTMinstname:Universidade Federal do Triangulo Mineiro (UFTM)instacron:UFTM2019-12-03T04:00:19Zoai:bdtd.uftm.edu.br:tede/929Biblioteca Digital de Teses e Dissertaçõeshttp://bdtd.uftm.edu.br/PUBhttp://bdtd.uftm.edu.br/oai/requestbdtd@uftm.edu.br||bdtd@uftm.edu.bropendoar:2024-04-24T09:58:49.372338Biblioteca Digital de Teses e Dissertações da UFTM - Universidade Federal do Triangulo Mineiro (UFTM)false
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