Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Detalhes bibliográficos
Autor(a) principal: Devi, C. Subathra
Data de Publicação: 2015
Outros Autores: Srinivasan, V. Mohana, Archana, B., Susan Roy, Steffi, Naine, S. Jemimah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/26263
Resumo: The current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.
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spelling Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3chitinaseBacillus cereus VITSD3antifungal activitychitinAspergilluspartial purification.Biological SciencesThe current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.EDUFU2015-05-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/2626310.14393/BJ-v31n3a2015-26263Bioscience Journal ; Vol. 31 No. 3 (2015): May/June; 960-968Bioscience Journal ; v. 31 n. 3 (2015): May/June; 960-9681981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/26263/16488Brazil; ContemporaryCopyright (c) 2015 C. Subathra Devi, V. Mohana Srinivasan, B. Archana, Steffi Susan Roy, S. Jemimah Nainehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDevi, C. SubathraSrinivasan, V. MohanaArchana, B.Susan Roy, SteffiNaine, S. Jemimah2022-05-26T16:30:34Zoai:ojs.www.seer.ufu.br:article/26263Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-26T16:30:34Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
title Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
spellingShingle Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
Devi, C. Subathra
chitinaseBacillus cereus VITSD3
antifungal activity
chitin
Aspergillus
partial purification.
Biological Sciences
title_short Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
title_full Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
title_fullStr Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
title_full_unstemmed Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
title_sort Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3
author Devi, C. Subathra
author_facet Devi, C. Subathra
Srinivasan, V. Mohana
Archana, B.
Susan Roy, Steffi
Naine, S. Jemimah
author_role author
author2 Srinivasan, V. Mohana
Archana, B.
Susan Roy, Steffi
Naine, S. Jemimah
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Devi, C. Subathra
Srinivasan, V. Mohana
Archana, B.
Susan Roy, Steffi
Naine, S. Jemimah
dc.subject.por.fl_str_mv chitinaseBacillus cereus VITSD3
antifungal activity
chitin
Aspergillus
partial purification.
Biological Sciences
topic chitinaseBacillus cereus VITSD3
antifungal activity
chitin
Aspergillus
partial purification.
Biological Sciences
description The current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/26263
10.14393/BJ-v31n3a2015-26263
url https://seer.ufu.br/index.php/biosciencejournal/article/view/26263
identifier_str_mv 10.14393/BJ-v31n3a2015-26263
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/26263/16488
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 31 No. 3 (2015): May/June; 960-968
Bioscience Journal ; v. 31 n. 3 (2015): May/June; 960-968
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
_version_ 1797069075274268672

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