Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/39998 |
Resumo: | Usually, weak inorganic acids have been used to disperse collagen as green solvents for fabricating kinds of biomaterials all the time. However, it is an open question how much the dissolving process preserves or alters the native structure of collagen till now. Herein, we have examined the effect of three different solvents (HAc, HCl, H3PO4) on the secondary structures of collagen, based on circular dichroism (CD) spectra of collagen from 185 to 260 nm together with CDNN programs. We have found that collagen almost completely preserved its triple helical structure in the three inorganic acids at pH=3.0 or so, which demonstrated that it was the concentration of free H+ in the above three solutions whose pH was fixed at 3.0 that can maintain an proper amount of surface charge on the collagen colloidal particles and appropriately loose the three-helix structure, which can not only lead to a better dispersion behavior, but also maximize the preservation of the integrity of the collagen structure. Although the fractions of kinds of secondary structures in collagen were different from all the three solvents based on CDNN data, which gave very similar results for each other. These results was tested for the first time in this work to estimate the secondary structures for collagen in the different common inorganic acids, which provides a new avenue for green collagen solvents to prepare collagen-based composite with well triple-helical structure for tissue engineering. |
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Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra Estimativa da estrutura de colágeno tipo I dissolvida em ácidos inorgânicos a partir de espectros de dicroísmo circulartype I collageninorganic acidthe triple helical structureCDBiological SciencesUsually, weak inorganic acids have been used to disperse collagen as green solvents for fabricating kinds of biomaterials all the time. However, it is an open question how much the dissolving process preserves or alters the native structure of collagen till now. Herein, we have examined the effect of three different solvents (HAc, HCl, H3PO4) on the secondary structures of collagen, based on circular dichroism (CD) spectra of collagen from 185 to 260 nm together with CDNN programs. We have found that collagen almost completely preserved its triple helical structure in the three inorganic acids at pH=3.0 or so, which demonstrated that it was the concentration of free H+ in the above three solutions whose pH was fixed at 3.0 that can maintain an proper amount of surface charge on the collagen colloidal particles and appropriately loose the three-helix structure, which can not only lead to a better dispersion behavior, but also maximize the preservation of the integrity of the collagen structure. Although the fractions of kinds of secondary structures in collagen were different from all the three solvents based on CDNN data, which gave very similar results for each other. These results was tested for the first time in this work to estimate the secondary structures for collagen in the different common inorganic acids, which provides a new avenue for green collagen solvents to prepare collagen-based composite with well triple-helical structure for tissue engineering.Habitualmente, os ácidos inorgânicos fracos têm sido usados para dispersar colágeno como solventes verdes para fabricar tipos de biomateriais o tempo todo. No entanto, é uma questão aberta quanto o processo dedissolução preserva ou altera a estrutura nativa do colágeno até agora. Aqui, examinamos o efeito de três solventes diferentes (HAc, HCl, H3PO4) nas estruturas secundárias de colágeno, com base em espectros de dicroísmo circular (CD) de colágeno de 185 a 260 nm em conjunto com programas CDNN. Descobrimos que o colágeno preservou quase completamente sua estrutura helicoidal tripla nos três ácidos inorgânicos a pH = 3,0 ou mais, o que demonstrou que foi a concentração de H+ livre nas três soluções acima cujo pH foi fixado em 3,0 que pode manter uma boa quantidade de carga superficial sobre as partículas coloidais de colágeno e destrói adequadamente a estrutura de três hélices, o que não só pode levar a um melhor comportamento de dispersão, mas também maximizar a preservação da integridade da estrutura de colágeno. Embora as frações de tipos de estruturas secundárias em colágeno fossem diferentes de todos os três solventescom base em dados CDNN, que deram resultados muito semelhantes entre si. Estes resultados foram testados pela primeira vez neste trabalho para estimar as estruturas secundárias para o colágeno nos diferentes ácidos inorgânicos comuns, o que fornece uma nova alternativa para solventes de colágeno verdes para preparar compósitos à base de colágeno com a estrutura helicoidal tripla para engenharia de tecidos.EDUFU2018-05-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3999810.14393/BJ-v34n3a2018-39998Bioscience Journal ; Vol. 34 No. 3 (2018): May/June; 778-789Bioscience Journal ; v. 34 n. 3 (2018): MAI/JUNE; 778-7891981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/39998/22243China; ContemporaryCopyright (c) 2018 Yuanyuan Zhou, Song Li, Dalu Wang, Yaguang Zhao, Xiaolin Leihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessZhou, YuanyuanLi, SongWang, DaluZhao, YaguangLei, Xiaolin2022-02-15T02:06:59Zoai:ojs.www.seer.ufu.br:article/39998Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-15T02:06:59Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra Estimativa da estrutura de colágeno tipo I dissolvida em ácidos inorgânicos a partir de espectros de dicroísmo circular |
title |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra |
spellingShingle |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra Zhou, Yuanyuan type I collagen inorganic acid the triple helical structure CD Biological Sciences |
title_short |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra |
title_full |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra |
title_fullStr |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra |
title_full_unstemmed |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra |
title_sort |
Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra |
author |
Zhou, Yuanyuan |
author_facet |
Zhou, Yuanyuan Li, Song Wang, Dalu Zhao, Yaguang Lei, Xiaolin |
author_role |
author |
author2 |
Li, Song Wang, Dalu Zhao, Yaguang Lei, Xiaolin |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Zhou, Yuanyuan Li, Song Wang, Dalu Zhao, Yaguang Lei, Xiaolin |
dc.subject.por.fl_str_mv |
type I collagen inorganic acid the triple helical structure CD Biological Sciences |
topic |
type I collagen inorganic acid the triple helical structure CD Biological Sciences |
description |
Usually, weak inorganic acids have been used to disperse collagen as green solvents for fabricating kinds of biomaterials all the time. However, it is an open question how much the dissolving process preserves or alters the native structure of collagen till now. Herein, we have examined the effect of three different solvents (HAc, HCl, H3PO4) on the secondary structures of collagen, based on circular dichroism (CD) spectra of collagen from 185 to 260 nm together with CDNN programs. We have found that collagen almost completely preserved its triple helical structure in the three inorganic acids at pH=3.0 or so, which demonstrated that it was the concentration of free H+ in the above three solutions whose pH was fixed at 3.0 that can maintain an proper amount of surface charge on the collagen colloidal particles and appropriately loose the three-helix structure, which can not only lead to a better dispersion behavior, but also maximize the preservation of the integrity of the collagen structure. Although the fractions of kinds of secondary structures in collagen were different from all the three solvents based on CDNN data, which gave very similar results for each other. These results was tested for the first time in this work to estimate the secondary structures for collagen in the different common inorganic acids, which provides a new avenue for green collagen solvents to prepare collagen-based composite with well triple-helical structure for tissue engineering. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-05-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/39998 10.14393/BJ-v34n3a2018-39998 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/39998 |
identifier_str_mv |
10.14393/BJ-v34n3a2018-39998 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/39998/22243 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Yuanyuan Zhou, Song Li, Dalu Wang, Yaguang Zhao, Xiaolin Lei https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Yuanyuan Zhou, Song Li, Dalu Wang, Yaguang Zhao, Xiaolin Lei https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
China; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 34 No. 3 (2018): May/June; 778-789 Bioscience Journal ; v. 34 n. 3 (2018): MAI/JUNE; 778-789 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069079108911104 |