New light sources for in-vitro potato micropropagation
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/26601 |
Resumo: | This research work objective was to optimize the micropropagation of potato cultivars through the use of new light sources in the growth rooms. Treatments consisted of three potato cultivars (Asterix, Catucha and Macaca), and five light sources (blue, green and red LEDs; Growlux and white fluorescent lamps). The explants consisted of nodal segments containing one bud, isolated from plantlets grown in vitro. The experimental design was completely randomized arranged in a 3x5 factorial, with eight replications. Each experimental unity consisted of a flask with five explants. Three 28-day consecutive subcultures were carried out in MS semi-solid medium, in growth-room under controlled conditions (temperature = 25+2 ºC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). At the end of each subculture, the bud number per plantlet, plantlet length and internode length were evaluated. After the third subculture, the concentrations of carotenoids and a- and b-chlorophylls were also determined. Different micropropagation efficiencies were found among potato cultivars grown in vitro conditions: 'Macaca' was the most and 'Catucha' the least responsive cultivar. The growth room light sources differently affected the potato plantlet development: red and green LEDs were the most and least recommended for plantlet development, based on the results of bud number per plantlet, plantlet length, and leaflet concentrations of a- and b-chlorophylls and carotenoids. |
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New light sources for in-vitro potato micropropagation Solanum tuberosumcultura de tecidosdiodos emissores de luzLEDsmicropropagaçãoAgricultural SciencesThis research work objective was to optimize the micropropagation of potato cultivars through the use of new light sources in the growth rooms. Treatments consisted of three potato cultivars (Asterix, Catucha and Macaca), and five light sources (blue, green and red LEDs; Growlux and white fluorescent lamps). The explants consisted of nodal segments containing one bud, isolated from plantlets grown in vitro. The experimental design was completely randomized arranged in a 3x5 factorial, with eight replications. Each experimental unity consisted of a flask with five explants. Three 28-day consecutive subcultures were carried out in MS semi-solid medium, in growth-room under controlled conditions (temperature = 25+2 ºC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). At the end of each subculture, the bud number per plantlet, plantlet length and internode length were evaluated. After the third subculture, the concentrations of carotenoids and a- and b-chlorophylls were also determined. Different micropropagation efficiencies were found among potato cultivars grown in vitro conditions: 'Macaca' was the most and 'Catucha' the least responsive cultivar. The growth room light sources differently affected the potato plantlet development: red and green LEDs were the most and least recommended for plantlet development, based on the results of bud number per plantlet, plantlet length, and leaflet concentrations of a- and b-chlorophylls and carotenoids. EDUFU2015-09-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/2660110.14393/BJ-v31n5a2015-26601Bioscience Journal ; Vol. 31 No. 5 (2015): Sept./Oct.; 1312-1318Bioscience Journal ; v. 31 n. 5 (2015): Sept./Oct.; 1312-13181981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/26601/17115Brazil; ContemporaryCopyright (c) 2015 Paulo Sergio Gomes Rocha, Roberto Pedroso de Oliveira, Walkyria Bueno Scivittarohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRocha, Paulo Sergio GomesOliveira, Roberto Pedroso deScivittaro, Walkyria Bueno2022-05-19T13:19:34Zoai:ojs.www.seer.ufu.br:article/26601Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-19T13:19:34Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
New light sources for in-vitro potato micropropagation |
title |
New light sources for in-vitro potato micropropagation |
spellingShingle |
New light sources for in-vitro potato micropropagation Rocha, Paulo Sergio Gomes Solanum tuberosum cultura de tecidos diodos emissores de luz LEDs micropropagação Agricultural Sciences |
title_short |
New light sources for in-vitro potato micropropagation |
title_full |
New light sources for in-vitro potato micropropagation |
title_fullStr |
New light sources for in-vitro potato micropropagation |
title_full_unstemmed |
New light sources for in-vitro potato micropropagation |
title_sort |
New light sources for in-vitro potato micropropagation |
author |
Rocha, Paulo Sergio Gomes |
author_facet |
Rocha, Paulo Sergio Gomes Oliveira, Roberto Pedroso de Scivittaro, Walkyria Bueno |
author_role |
author |
author2 |
Oliveira, Roberto Pedroso de Scivittaro, Walkyria Bueno |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rocha, Paulo Sergio Gomes Oliveira, Roberto Pedroso de Scivittaro, Walkyria Bueno |
dc.subject.por.fl_str_mv |
Solanum tuberosum cultura de tecidos diodos emissores de luz LEDs micropropagação Agricultural Sciences |
topic |
Solanum tuberosum cultura de tecidos diodos emissores de luz LEDs micropropagação Agricultural Sciences |
description |
This research work objective was to optimize the micropropagation of potato cultivars through the use of new light sources in the growth rooms. Treatments consisted of three potato cultivars (Asterix, Catucha and Macaca), and five light sources (blue, green and red LEDs; Growlux and white fluorescent lamps). The explants consisted of nodal segments containing one bud, isolated from plantlets grown in vitro. The experimental design was completely randomized arranged in a 3x5 factorial, with eight replications. Each experimental unity consisted of a flask with five explants. Three 28-day consecutive subcultures were carried out in MS semi-solid medium, in growth-room under controlled conditions (temperature = 25+2 ºC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). At the end of each subculture, the bud number per plantlet, plantlet length and internode length were evaluated. After the third subculture, the concentrations of carotenoids and a- and b-chlorophylls were also determined. Different micropropagation efficiencies were found among potato cultivars grown in vitro conditions: 'Macaca' was the most and 'Catucha' the least responsive cultivar. The growth room light sources differently affected the potato plantlet development: red and green LEDs were the most and least recommended for plantlet development, based on the results of bud number per plantlet, plantlet length, and leaflet concentrations of a- and b-chlorophylls and carotenoids. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/26601 10.14393/BJ-v31n5a2015-26601 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/26601 |
identifier_str_mv |
10.14393/BJ-v31n5a2015-26601 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/26601/17115 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Paulo Sergio Gomes Rocha, Roberto Pedroso de Oliveira, Walkyria Bueno Scivittaro https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Paulo Sergio Gomes Rocha, Roberto Pedroso de Oliveira, Walkyria Bueno Scivittaro https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 31 No. 5 (2015): Sept./Oct.; 1312-1318 Bioscience Journal ; v. 31 n. 5 (2015): Sept./Oct.; 1312-1318 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069075345571840 |