Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/35010 |
Resumo: | The standard physiological parameters for assessing stress physiology, of plants, such as chlorophyll index and photosystem II fluorescence, are essential for measuring reactions of plants to stress conditions. To help standardize the parameters for chlorophyll indices of chlorophylls a, b, and total and the fluorescence of photosystem II (Fv/Fm), which are physiological indicators of stress conditions, 6-mo-old seedlings of Persea americana Mill. cv. 'Duke7' and 'Toro canyon', were evaluated under shade house conditions. For each plant, chlorophyll indices were measured from the second through the fourteenth fully expanded leaves. Fluorescence was measured in the third, fifth, seventh, and ninth fully expanded leaf, and determined in function of both, time and intensity of the exposure light source, and the time for dark pre-acclimation of the leaf; was also compared right and left sides of the leaves. Â Chlorophyll indices were not different between the left and right sides of the leaves, but were different between varieties with 'Duke 7' having the highest value from the eighth leaf, while leaves tested for 'Toro canyon' did not show a difference. For Fv/Fm, there was an interaction among the three factors in both cultivars. A prolonged exposure time (nine seconds), short time for acclimation to darkness, and low intensity of exposure did not induce maximum fluorescence levels. On both avocado cultivars, exposure to maximum light intensity for 7 seconds after 25 to 30 minutes of acclimation to darkness, was the most favorable combination allowing the measurement of photosynthetic efficiency. |
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Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) Parâmetros para a estandarização de índices de clorofila e da eficiência quantica do ps II em abacateiro (Persea americana Mill)Avocadochlorophyll indexFv/Fmphotosystem IIphysiological parametersAgricultural SciencesThe standard physiological parameters for assessing stress physiology, of plants, such as chlorophyll index and photosystem II fluorescence, are essential for measuring reactions of plants to stress conditions. To help standardize the parameters for chlorophyll indices of chlorophylls a, b, and total and the fluorescence of photosystem II (Fv/Fm), which are physiological indicators of stress conditions, 6-mo-old seedlings of Persea americana Mill. cv. 'Duke7' and 'Toro canyon', were evaluated under shade house conditions. For each plant, chlorophyll indices were measured from the second through the fourteenth fully expanded leaves. Fluorescence was measured in the third, fifth, seventh, and ninth fully expanded leaf, and determined in function of both, time and intensity of the exposure light source, and the time for dark pre-acclimation of the leaf; was also compared right and left sides of the leaves. Â Chlorophyll indices were not different between the left and right sides of the leaves, but were different between varieties with 'Duke 7' having the highest value from the eighth leaf, while leaves tested for 'Toro canyon' did not show a difference. For Fv/Fm, there was an interaction among the three factors in both cultivars. A prolonged exposure time (nine seconds), short time for acclimation to darkness, and low intensity of exposure did not induce maximum fluorescence levels. On both avocado cultivars, exposure to maximum light intensity for 7 seconds after 25 to 30 minutes of acclimation to darkness, was the most favorable combination allowing the measurement of photosynthetic efficiency.Os parâmetros padronizados para avaliação do estresse fisiológico de plantas, tais como índice de clorofila: fluorescência do fotossistema II, são essenciais para medir as reações de plantas a condições de estresse. Para auxiliar na padronização dos parâmetros para os índices de clorofila de clorofilas a, b e total e a fluorescência do fotossistema II (Fv / Fm), que são indicadores fisiológicos de condições de estresse, mudas de 6 meses de idade, de Persea americana Mill. Cv. 'Duke7' e 'Toro canyon' foram avaliadas sob condições casa de vegetação. Para cada planta, índices de clorofila foram medidos da segunda até a décima quarta folha completamente expandida. A fluorescência foi medida na terceira, quinta, sétima e nona folha totalmente expandida, e determinada em função do tempo e intensidade de exposição à fonte de luz, assim como do tempo para pré-adaptação da folha no escuro; também foram comparados entre o lado direito e esquerdo das folhas. Os índices de clorofila não foram diferentes entre os lados direito e esquerdo das folhas, mas foram entre diferentes variedades com 'Duke 7' tendo o valor mais alto a partir da oitava folha, enquanto que as de 'Toro canyon' não mostraram diferença. Para Fv/Fm, houve interação entre os três fatores em ambas as cultivares. O tempo prolongado de exposição (nove segundos), tempo curto para aclimatação no escuro, e baixa intensidade de exposição não induziu níveis máximos de fluorescência. Em ambas as cultivares de abacate, 100% de exposição à intensidade máxima de luz durante 7 segundos, após 25 a 30 minutos de adaptação no escuro, foi a combinação mais adequada para a medição da eficiência fotossintética.EDUFU2017-07-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3501010.14393/BJ-v33n4a2017-35010Bioscience Journal ; Vol. 33 No. 4 (2017): July/Aug.; 968-978Bioscience Journal ; v. 33 n. 4 (2017): July/Aug.; 968-9781981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/35010/20665Brazil; ContemporaryCopyright (c) 2017 Edwin Antonio Gutierrez Rodrigues, Mauricio Panizzi Penariol, Pedro Luis Alves da Costa Aguiar, Rita de Cassia Panizzi, Renata Aparecida de Andradehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGutierrez Rodrigues, Edwin AntonioPenariol, Mauricio PanizziAguiar, Pedro Luis Alves da CostaPanizzi, Rita de CassiaAndrade, Renata Aparecida de2022-02-14T01:38:23Zoai:ojs.www.seer.ufu.br:article/35010Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-14T01:38:23Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) Parâmetros para a estandarização de índices de clorofila e da eficiência quantica do ps II em abacateiro (Persea americana Mill) |
title |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) |
spellingShingle |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) Gutierrez Rodrigues, Edwin Antonio Avocado chlorophyll index Fv/Fm photosystem II physiological parameters Agricultural Sciences |
title_short |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) |
title_full |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) |
title_fullStr |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) |
title_full_unstemmed |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) |
title_sort |
Parameters for the standardization of chlorophyll and assessment of PS II quantum efficiency for avocado (Persea americana Mill) |
author |
Gutierrez Rodrigues, Edwin Antonio |
author_facet |
Gutierrez Rodrigues, Edwin Antonio Penariol, Mauricio Panizzi Aguiar, Pedro Luis Alves da Costa Panizzi, Rita de Cassia Andrade, Renata Aparecida de |
author_role |
author |
author2 |
Penariol, Mauricio Panizzi Aguiar, Pedro Luis Alves da Costa Panizzi, Rita de Cassia Andrade, Renata Aparecida de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gutierrez Rodrigues, Edwin Antonio Penariol, Mauricio Panizzi Aguiar, Pedro Luis Alves da Costa Panizzi, Rita de Cassia Andrade, Renata Aparecida de |
dc.subject.por.fl_str_mv |
Avocado chlorophyll index Fv/Fm photosystem II physiological parameters Agricultural Sciences |
topic |
Avocado chlorophyll index Fv/Fm photosystem II physiological parameters Agricultural Sciences |
description |
The standard physiological parameters for assessing stress physiology, of plants, such as chlorophyll index and photosystem II fluorescence, are essential for measuring reactions of plants to stress conditions. To help standardize the parameters for chlorophyll indices of chlorophylls a, b, and total and the fluorescence of photosystem II (Fv/Fm), which are physiological indicators of stress conditions, 6-mo-old seedlings of Persea americana Mill. cv. 'Duke7' and 'Toro canyon', were evaluated under shade house conditions. For each plant, chlorophyll indices were measured from the second through the fourteenth fully expanded leaves. Fluorescence was measured in the third, fifth, seventh, and ninth fully expanded leaf, and determined in function of both, time and intensity of the exposure light source, and the time for dark pre-acclimation of the leaf; was also compared right and left sides of the leaves. Â Chlorophyll indices were not different between the left and right sides of the leaves, but were different between varieties with 'Duke 7' having the highest value from the eighth leaf, while leaves tested for 'Toro canyon' did not show a difference. For Fv/Fm, there was an interaction among the three factors in both cultivars. A prolonged exposure time (nine seconds), short time for acclimation to darkness, and low intensity of exposure did not induce maximum fluorescence levels. On both avocado cultivars, exposure to maximum light intensity for 7 seconds after 25 to 30 minutes of acclimation to darkness, was the most favorable combination allowing the measurement of photosynthetic efficiency. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/35010 10.14393/BJ-v33n4a2017-35010 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/35010 |
identifier_str_mv |
10.14393/BJ-v33n4a2017-35010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/35010/20665 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 33 No. 4 (2017): July/Aug.; 968-978 Bioscience Journal ; v. 33 n. 4 (2017): July/Aug.; 968-978 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069077113470976 |