Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments

Detalhes bibliográficos
Autor(a) principal: Wei, Shiping
Data de Publicação: 2018
Outros Autores: Liu, Beibei, Cui, Hongpeng, Fan, Rui, Guo, Ce, Sun, Mingxue
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/41757
Resumo: A total of 114 moderately halophilic bacteria were isolated from marine sediment environments. The isolates are belonged to 23 species based on the 16S rRNA sequence analysis. 63, 52, 47, 57, 74, 15 and 4 isolates are able to produce protease, amylase, lipase, pectinase, pulluanase, xylanase, cellulase, respectively. Combined hydrolytic enzyme activity analysis show that 15 strains present 1 hydrolytic activity, 32 strains present 2 hydrolytic activities, 21 strains present 3 hydrolytic activities, 26 strains present 4 hydrolytic activities, 11 strains present 5 hydrolytic activities and 2 strains present 6 hydrolytic activities. Hydrolase activities are widely distributed in a variety of species. The highest rates for production of protease, amylase, lipase, pectinase, pullanase, xylanase and cellulase were observed in species of B. baekryungensis, Hallobacillus sp., B. pumilus, B. megaterium or P. chungwhensis, B. amyloliquefaciens, B. pumilus, B. baekryungensis, respectively. However, the higher activities of protease, pectinase and pulluanase are frequently produced by the species of Halomonas sp. B. amyloliquefaciens or P. chungwhensis, and Vibrio sp. respectively. This investigation show that the diversity of halophilic bacteria from marine sediments could serve as a potential source of hydrolytic enzymes for industrial applications.
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spelling Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments Diversidade de bactérias cultiváveis moderadamente halofílicas que produzem enzimas hidrolíticas extracelulares de sedimentos marinhosmoderately halophilic bacteriahydrolytic enzymemarine sedimentBiological SciencesA total of 114 moderately halophilic bacteria were isolated from marine sediment environments. The isolates are belonged to 23 species based on the 16S rRNA sequence analysis. 63, 52, 47, 57, 74, 15 and 4 isolates are able to produce protease, amylase, lipase, pectinase, pulluanase, xylanase, cellulase, respectively. Combined hydrolytic enzyme activity analysis show that 15 strains present 1 hydrolytic activity, 32 strains present 2 hydrolytic activities, 21 strains present 3 hydrolytic activities, 26 strains present 4 hydrolytic activities, 11 strains present 5 hydrolytic activities and 2 strains present 6 hydrolytic activities. Hydrolase activities are widely distributed in a variety of species. The highest rates for production of protease, amylase, lipase, pectinase, pullanase, xylanase and cellulase were observed in species of B. baekryungensis, Hallobacillus sp., B. pumilus, B. megaterium or P. chungwhensis, B. amyloliquefaciens, B. pumilus, B. baekryungensis, respectively. However, the higher activities of protease, pectinase and pulluanase are frequently produced by the species of Halomonas sp. B. amyloliquefaciens or P. chungwhensis, and Vibrio sp. respectively. This investigation show that the diversity of halophilic bacteria from marine sediments could serve as a potential source of hydrolytic enzymes for industrial applications.Um total de 114 bactérias moderadamente halofílicas foram isoladas de ambientes de sedimentos marinhos. Os isolados pertencem a 23 espécies com base na análise da sequência 16S rRNA. 63, 52, 47, 57, 74, 15 e 4 isolados são capazes de produzir protease, amilase, lipase, pectinase, pululanase, xilanase, celulase, respectivamente. A análise da atividade enzimática hidrolítica combinada mostra que 15 cepas apresentam 1 atividade hidrolítica, 32 cepas apresentam 2 atividades hidrolíticas, 21 cepas apresentam 3 atividades hidrolíticas, 26 cepas apresentam 4 atividades hidrolíticas, 11 cepas apresentam 5 atividades hidrolíticas e 2 cepas apresentam 6 atividades hidrolíticas. Atividades de hidrolase são amplamente distribuídas em uma variedade de espécies. As maiores taxas de produção de protease, amilase, lipase, pectinase, pululanase, xilanase e celulase foram observadas em espécies de B. baekryungensis, Hallobacillus sp., B. pumilus, B. megaterium ou P. chungwhensis, B. amyloliquefaciens, B. pumilus, B. baekryungensis, respectivamente. Noentanto, as atividades mais elevadas de protease, pectinase e pululanase são freqüentemente produzidas pelas espécies de Halomonas sp. B. amyloliquefaciens ou P. chungwhensis e Vibrio sp. respectivamente. Esta investigação mostra que a diversidade de bactérias halofílicas de sedimentos marinhos pode servir como uma fonte potencial de enzimas hidrolíticas para aplicações industriais.EDUFU2018-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4175710.14393/BJ-v34n6a2018-41757Bioscience Journal ; Vol. 34 No. 6 (2018): Nov./Dec.; 1724-1732Bioscience Journal ; v. 34 n. 6 (2018): Nov./Dec.; 1724-17321981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/41757/24862Brazil; ContemporaryCopyright (c) 2018 Shiping Wei, Beibei Liu, Hongpeng Cui, Rui Fan, Ce Guo, Mingxue Sunhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessWei, ShipingLiu, BeibeiCui, HongpengFan, RuiGuo, CeSun, Mingxue2022-02-08T01:48:45Zoai:ojs.www.seer.ufu.br:article/41757Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-08T01:48:45Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
Diversidade de bactérias cultiváveis moderadamente halofílicas que produzem enzimas hidrolíticas extracelulares de sedimentos marinhos
title Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
spellingShingle Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
Wei, Shiping
moderately halophilic bacteria
hydrolytic enzyme
marine sediment
Biological Sciences
title_short Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
title_full Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
title_fullStr Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
title_full_unstemmed Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
title_sort Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments
author Wei, Shiping
author_facet Wei, Shiping
Liu, Beibei
Cui, Hongpeng
Fan, Rui
Guo, Ce
Sun, Mingxue
author_role author
author2 Liu, Beibei
Cui, Hongpeng
Fan, Rui
Guo, Ce
Sun, Mingxue
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Wei, Shiping
Liu, Beibei
Cui, Hongpeng
Fan, Rui
Guo, Ce
Sun, Mingxue
dc.subject.por.fl_str_mv moderately halophilic bacteria
hydrolytic enzyme
marine sediment
Biological Sciences
topic moderately halophilic bacteria
hydrolytic enzyme
marine sediment
Biological Sciences
description A total of 114 moderately halophilic bacteria were isolated from marine sediment environments. The isolates are belonged to 23 species based on the 16S rRNA sequence analysis. 63, 52, 47, 57, 74, 15 and 4 isolates are able to produce protease, amylase, lipase, pectinase, pulluanase, xylanase, cellulase, respectively. Combined hydrolytic enzyme activity analysis show that 15 strains present 1 hydrolytic activity, 32 strains present 2 hydrolytic activities, 21 strains present 3 hydrolytic activities, 26 strains present 4 hydrolytic activities, 11 strains present 5 hydrolytic activities and 2 strains present 6 hydrolytic activities. Hydrolase activities are widely distributed in a variety of species. The highest rates for production of protease, amylase, lipase, pectinase, pullanase, xylanase and cellulase were observed in species of B. baekryungensis, Hallobacillus sp., B. pumilus, B. megaterium or P. chungwhensis, B. amyloliquefaciens, B. pumilus, B. baekryungensis, respectively. However, the higher activities of protease, pectinase and pulluanase are frequently produced by the species of Halomonas sp. B. amyloliquefaciens or P. chungwhensis, and Vibrio sp. respectively. This investigation show that the diversity of halophilic bacteria from marine sediments could serve as a potential source of hydrolytic enzymes for industrial applications.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/41757
10.14393/BJ-v34n6a2018-41757
url https://seer.ufu.br/index.php/biosciencejournal/article/view/41757
identifier_str_mv 10.14393/BJ-v34n6a2018-41757
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/41757/24862
dc.rights.driver.fl_str_mv Copyright (c) 2018 Shiping Wei, Beibei Liu, Hongpeng Cui, Rui Fan, Ce Guo, Mingxue Sun
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Shiping Wei, Beibei Liu, Hongpeng Cui, Rui Fan, Ce Guo, Mingxue Sun
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 34 No. 6 (2018): Nov./Dec.; 1724-1732
Bioscience Journal ; v. 34 n. 6 (2018): Nov./Dec.; 1724-1732
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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