Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/67616 |
Resumo: | The current study aimed to detect the antimicrobial effect of cell free supernatant (CFS) of Lactobacillus plantarum strain against Gram +ve and Gram –ve bacterial strains. The strain of Lactobacillus plantarum was isolated using selective media MRS agar. The strain was characterized on the basis of the gram staining, colony morphology, the biochemical tests and the DNA sequencing based method of 16S ribotyping. A total of four test strains (The three already isolated and reported strains (E.coli, S. aureus and B. subtilis) and the one recently identified novel strain (B. cereus) were used for the analysis of antagonistic activity of bacteriocin produced by L. plantarum strain. The CFS of L. plantarum showed zone of inhibition against all the test strains (Gram +ve and Gram –ve bacteria). The conditions favoring the growth of bacteria were associated with the antimicrobial efficacy of CFS. Bacteriocin activity of CFS remained effective after exposure to temperature stress. Wide range of antagonistic potential of CFS of L. plantarum provides an alternative for antibiotics in pharmaceutical industry. Heat resistant feature of bacteriocin suggests its application in food industry. |
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Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strainsAntimicrobial activityBacteriocinBacillus cereusLactobacillus plantarumMolecular identification.BioscienceThe current study aimed to detect the antimicrobial effect of cell free supernatant (CFS) of Lactobacillus plantarum strain against Gram +ve and Gram –ve bacterial strains. The strain of Lactobacillus plantarum was isolated using selective media MRS agar. The strain was characterized on the basis of the gram staining, colony morphology, the biochemical tests and the DNA sequencing based method of 16S ribotyping. A total of four test strains (The three already isolated and reported strains (E.coli, S. aureus and B. subtilis) and the one recently identified novel strain (B. cereus) were used for the analysis of antagonistic activity of bacteriocin produced by L. plantarum strain. The CFS of L. plantarum showed zone of inhibition against all the test strains (Gram +ve and Gram –ve bacteria). The conditions favoring the growth of bacteria were associated with the antimicrobial efficacy of CFS. Bacteriocin activity of CFS remained effective after exposure to temperature stress. Wide range of antagonistic potential of CFS of L. plantarum provides an alternative for antibiotics in pharmaceutical industry. Heat resistant feature of bacteriocin suggests its application in food industry.Universidade Federal de Uberlândia2023-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6761610.14393/BJ-v39n0a2023-67616Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39061Bioscience Journal ; v. 39 (2023): Continuous Publication; e390611981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/67616/35918Pakistan; Contemporary Copyright (c) 2023 Samreen Akhtar, Syed Kashif Nawazhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAkhtar, SamreenNawaz, Syed Kashif2024-01-31T19:16:18Zoai:ojs.www.seer.ufu.br:article/67616Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-01-31T19:16:18Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
title |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
spellingShingle |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains Akhtar, Samreen Antimicrobial activity Bacteriocin Bacillus cereus Lactobacillus plantarum Molecular identification. Bioscience |
title_short |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
title_full |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
title_fullStr |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
title_full_unstemmed |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
title_sort |
Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains |
author |
Akhtar, Samreen |
author_facet |
Akhtar, Samreen Nawaz, Syed Kashif |
author_role |
author |
author2 |
Nawaz, Syed Kashif |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Akhtar, Samreen Nawaz, Syed Kashif |
dc.subject.por.fl_str_mv |
Antimicrobial activity Bacteriocin Bacillus cereus Lactobacillus plantarum Molecular identification. Bioscience |
topic |
Antimicrobial activity Bacteriocin Bacillus cereus Lactobacillus plantarum Molecular identification. Bioscience |
description |
The current study aimed to detect the antimicrobial effect of cell free supernatant (CFS) of Lactobacillus plantarum strain against Gram +ve and Gram –ve bacterial strains. The strain of Lactobacillus plantarum was isolated using selective media MRS agar. The strain was characterized on the basis of the gram staining, colony morphology, the biochemical tests and the DNA sequencing based method of 16S ribotyping. A total of four test strains (The three already isolated and reported strains (E.coli, S. aureus and B. subtilis) and the one recently identified novel strain (B. cereus) were used for the analysis of antagonistic activity of bacteriocin produced by L. plantarum strain. The CFS of L. plantarum showed zone of inhibition against all the test strains (Gram +ve and Gram –ve bacteria). The conditions favoring the growth of bacteria were associated with the antimicrobial efficacy of CFS. Bacteriocin activity of CFS remained effective after exposure to temperature stress. Wide range of antagonistic potential of CFS of L. plantarum provides an alternative for antibiotics in pharmaceutical industry. Heat resistant feature of bacteriocin suggests its application in food industry. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/67616 10.14393/BJ-v39n0a2023-67616 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/67616 |
identifier_str_mv |
10.14393/BJ-v39n0a2023-67616 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/67616/35918 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Samreen Akhtar, Syed Kashif Nawaz https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Samreen Akhtar, Syed Kashif Nawaz https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Pakistan; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39061 Bioscience Journal ; v. 39 (2023): Continuous Publication; e39061 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069065804578816 |