Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains

Detalhes bibliográficos
Autor(a) principal: Akhtar, Samreen
Data de Publicação: 2023
Outros Autores: Nawaz, Syed Kashif
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/67616
Resumo: The current study aimed to detect the antimicrobial effect of cell free supernatant (CFS) of Lactobacillus plantarum strain against Gram +ve and Gram –ve bacterial strains. The strain of Lactobacillus plantarum was isolated using selective media MRS agar. The strain was characterized on the basis of the gram staining, colony morphology, the biochemical tests and the DNA sequencing based method of 16S ribotyping. A total of four test strains (The three already isolated and reported strains (E.coli, S. aureus and B. subtilis) and the one recently identified  novel strain (B. cereus) were used for the analysis of antagonistic activity of bacteriocin produced by L. plantarum strain. The CFS of L. plantarum showed zone of inhibition against all the test strains (Gram +ve and Gram –ve bacteria). The conditions favoring the growth of bacteria were associated with the antimicrobial efficacy of CFS. Bacteriocin activity of CFS remained effective after exposure to temperature stress.  Wide range of antagonistic potential of CFS of L. plantarum provides an alternative for antibiotics in pharmaceutical industry. Heat resistant feature of bacteriocin suggests its application in food industry.
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spelling Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strainsAntimicrobial activityBacteriocinBacillus cereusLactobacillus plantarumMolecular identification.BioscienceThe current study aimed to detect the antimicrobial effect of cell free supernatant (CFS) of Lactobacillus plantarum strain against Gram +ve and Gram –ve bacterial strains. The strain of Lactobacillus plantarum was isolated using selective media MRS agar. The strain was characterized on the basis of the gram staining, colony morphology, the biochemical tests and the DNA sequencing based method of 16S ribotyping. A total of four test strains (The three already isolated and reported strains (E.coli, S. aureus and B. subtilis) and the one recently identified  novel strain (B. cereus) were used for the analysis of antagonistic activity of bacteriocin produced by L. plantarum strain. The CFS of L. plantarum showed zone of inhibition against all the test strains (Gram +ve and Gram –ve bacteria). The conditions favoring the growth of bacteria were associated with the antimicrobial efficacy of CFS. Bacteriocin activity of CFS remained effective after exposure to temperature stress.  Wide range of antagonistic potential of CFS of L. plantarum provides an alternative for antibiotics in pharmaceutical industry. Heat resistant feature of bacteriocin suggests its application in food industry.Universidade Federal de Uberlândia2023-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6761610.14393/BJ-v39n0a2023-67616Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39061Bioscience Journal ; v. 39 (2023): Continuous Publication; e390611981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/67616/35918Pakistan; Contemporary Copyright (c) 2023 Samreen Akhtar, Syed Kashif Nawazhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAkhtar, SamreenNawaz, Syed Kashif2024-01-31T19:16:18Zoai:ojs.www.seer.ufu.br:article/67616Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-01-31T19:16:18Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
title Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
spellingShingle Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
Akhtar, Samreen
Antimicrobial activity
Bacteriocin
Bacillus cereus
Lactobacillus plantarum
Molecular identification.
Bioscience
title_short Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
title_full Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
title_fullStr Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
title_full_unstemmed Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
title_sort Antimicrobial efficacy of Lactobacillus plantarum strain against the B. cereus, B. subtilis, S. aureus and E.coli strains
author Akhtar, Samreen
author_facet Akhtar, Samreen
Nawaz, Syed Kashif
author_role author
author2 Nawaz, Syed Kashif
author2_role author
dc.contributor.author.fl_str_mv Akhtar, Samreen
Nawaz, Syed Kashif
dc.subject.por.fl_str_mv Antimicrobial activity
Bacteriocin
Bacillus cereus
Lactobacillus plantarum
Molecular identification.
Bioscience
topic Antimicrobial activity
Bacteriocin
Bacillus cereus
Lactobacillus plantarum
Molecular identification.
Bioscience
description The current study aimed to detect the antimicrobial effect of cell free supernatant (CFS) of Lactobacillus plantarum strain against Gram +ve and Gram –ve bacterial strains. The strain of Lactobacillus plantarum was isolated using selective media MRS agar. The strain was characterized on the basis of the gram staining, colony morphology, the biochemical tests and the DNA sequencing based method of 16S ribotyping. A total of four test strains (The three already isolated and reported strains (E.coli, S. aureus and B. subtilis) and the one recently identified  novel strain (B. cereus) were used for the analysis of antagonistic activity of bacteriocin produced by L. plantarum strain. The CFS of L. plantarum showed zone of inhibition against all the test strains (Gram +ve and Gram –ve bacteria). The conditions favoring the growth of bacteria were associated with the antimicrobial efficacy of CFS. Bacteriocin activity of CFS remained effective after exposure to temperature stress.  Wide range of antagonistic potential of CFS of L. plantarum provides an alternative for antibiotics in pharmaceutical industry. Heat resistant feature of bacteriocin suggests its application in food industry.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/67616
10.14393/BJ-v39n0a2023-67616
url https://seer.ufu.br/index.php/biosciencejournal/article/view/67616
identifier_str_mv 10.14393/BJ-v39n0a2023-67616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/67616/35918
dc.rights.driver.fl_str_mv Copyright (c) 2023 Samreen Akhtar, Syed Kashif Nawaz
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Samreen Akhtar, Syed Kashif Nawaz
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Pakistan; Contemporary
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
publisher.none.fl_str_mv Universidade Federal de Uberlândia
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39061
Bioscience Journal ; v. 39 (2023): Continuous Publication; e39061
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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