In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/38776 |
Resumo: | Orchids are known for the beauty, exuberance, and color of their flowers and therefore represent one of the most coveted ornamental plants. Due to their high commercial value, several illegal practices of removal and commercialization have led to the verge of extinction. This study aimed to develop protocols for in vitro conservation under slow growth and acclimatization of species of the subfamily Epidendroideae (Orchidaceae), from the state of Sergipe, Brazil. Two experiments were conducted: the first one tested four concentrations of MS (MURASHIGE and SKOOG, 1962) (25%, 50%, 75%, 100%), three orchid species (Catasetum macrocarpum, Oeceoclades maculata, and Polystachya estrellensis), and two temperatures (18 and 25ºC); the second one tested three combinations of carbon sources and osmotic regulators (20 g L-1 sucrose; 10 g L-1 sucrose + 5 g L-1 de mannitol; 10 g L-1 sucrose + 5 g L-1 sorbitol) and the same three species and two temperatures. For the in vitro conservation of C. macrocarpum, O. maculata, and P. estrellensis over 450 days, the use of 25% of MS salts or 20 g.L-1 sucrose at 25ºC is recommended. C. macrocarpum seedlings were acclimatized using sand + shredded pine bark + worm castings at a ratio of 2:2:1 (w:w:w). The use of sand alone is recommended for the acclimatization of O. maculata. |
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In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, BrazilConservação in vitro e aclimatização de Epidendroideae (Orchidaceae) de Sergipe, BrasilCatasetum macrocarpumOeceoclades maculatePolystachya estrellensisSlow growthSubstrateAgricultural SciencesOrchids are known for the beauty, exuberance, and color of their flowers and therefore represent one of the most coveted ornamental plants. Due to their high commercial value, several illegal practices of removal and commercialization have led to the verge of extinction. This study aimed to develop protocols for in vitro conservation under slow growth and acclimatization of species of the subfamily Epidendroideae (Orchidaceae), from the state of Sergipe, Brazil. Two experiments were conducted: the first one tested four concentrations of MS (MURASHIGE and SKOOG, 1962) (25%, 50%, 75%, 100%), three orchid species (Catasetum macrocarpum, Oeceoclades maculata, and Polystachya estrellensis), and two temperatures (18 and 25ºC); the second one tested three combinations of carbon sources and osmotic regulators (20 g L-1 sucrose; 10 g L-1 sucrose + 5 g L-1 de mannitol; 10 g L-1 sucrose + 5 g L-1 sorbitol) and the same three species and two temperatures. For the in vitro conservation of C. macrocarpum, O. maculata, and P. estrellensis over 450 days, the use of 25% of MS salts or 20 g.L-1 sucrose at 25ºC is recommended. C. macrocarpum seedlings were acclimatized using sand + shredded pine bark + worm castings at a ratio of 2:2:1 (w:w:w). The use of sand alone is recommended for the acclimatization of O. maculata.As orquídeas são conhecidas pela beleza, exuberância e cores de suas flores e por isso representam uma das plantas ornamentais mais cobiçadas. Devido ao alto valor comercial, muitas são as práticas ilegais de retirada e comercialização de orquídeas, o que leva as espécies à beira de extinção. O objetivo deste estudo foi desenvolver protocolos para a conservação in vitro sob crescimento lento e aclimatação de espécies da subfamília Epidendroideae (Orchidaceae) do Estado de Sergipe, Brasil. Dois experimentos foram realizados: o primeiro ensaio testou quatro concentrações dos sais MS (MURASHIGE e SKOOG, 1962) (25, 50, 75 e 100%), três espécies (Catasetum macrocarpum, Oeceoclades maculata e Polystachya estrellensis) e duas temperaturas (18 e 25ºC); o segundo ensaio testou três combinações de fontes de carbono e reguladores osmóticos (20 g L-1 de sacarose; 10 g L-1 de sacarose + 5 g L-1 manitol; 10 g L-1 de sacarose + 5 g L-1 sorbitol), três espécies (C. macrocarpum, O. maculata e P. estrellensis) e duas temperaturas (18 e 25ºC). Para executar a aclimatação, oito substratos foram testados para as espécies C. macrocarpum e O. maculata. Para realizar a conservação in vitro de C. macrocarpum, O. maculata e P. estrellensis ao longo de 450 dias, recomenda-se a utilização 25% dos sais de MS e de 20 g L-1 de sacarose a 25°C. Mudas de C. macrocarpum foram aclimatizadas utilizando areia + casca de pinus + húmus de minhoca (2:2:1). Recomendase a utilização de areia para aclimatização de O. maculata.EDUFU2019-04-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3877610.14393/BJ-v35n2a20198-38776Bioscience Journal ; Vol. 35 No. 2 (2019): Mar./Apr.; 356-366Bioscience Journal ; v. 35 n. 2 (2019): Mar./Apr.; 356-3661981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/38776/25781Brazil; ContemporaryCopyright (c) 2019 Thays Saynara Alves Menezes, Maria de Fátima Arrigoni- Blank, Andrea Santos da Costa, Rosana Barroso Feitosa-Alcantara, Arie Fitzgerald Blank, José Magno Queiroz Luzhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMenezes, Thays Saynara AlvesBlank, Maria de Fátima Arrigoni-Costa, Andrea Santos daFeitosa-Alcantara, Rosana BarrosoBlank, Arie FitzgeraldLuz, José Magno Queiroz2022-02-02T04:38:22Zoai:ojs.www.seer.ufu.br:article/38776Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-02T04:38:22Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil Conservação in vitro e aclimatização de Epidendroideae (Orchidaceae) de Sergipe, Brasil |
title |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil |
spellingShingle |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil Menezes, Thays Saynara Alves Catasetum macrocarpum Oeceoclades maculate Polystachya estrellensis Slow growth Substrate Agricultural Sciences |
title_short |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil |
title_full |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil |
title_fullStr |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil |
title_full_unstemmed |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil |
title_sort |
In vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil |
author |
Menezes, Thays Saynara Alves |
author_facet |
Menezes, Thays Saynara Alves Blank, Maria de Fátima Arrigoni- Costa, Andrea Santos da Feitosa-Alcantara, Rosana Barroso Blank, Arie Fitzgerald Luz, José Magno Queiroz |
author_role |
author |
author2 |
Blank, Maria de Fátima Arrigoni- Costa, Andrea Santos da Feitosa-Alcantara, Rosana Barroso Blank, Arie Fitzgerald Luz, José Magno Queiroz |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Menezes, Thays Saynara Alves Blank, Maria de Fátima Arrigoni- Costa, Andrea Santos da Feitosa-Alcantara, Rosana Barroso Blank, Arie Fitzgerald Luz, José Magno Queiroz |
dc.subject.por.fl_str_mv |
Catasetum macrocarpum Oeceoclades maculate Polystachya estrellensis Slow growth Substrate Agricultural Sciences |
topic |
Catasetum macrocarpum Oeceoclades maculate Polystachya estrellensis Slow growth Substrate Agricultural Sciences |
description |
Orchids are known for the beauty, exuberance, and color of their flowers and therefore represent one of the most coveted ornamental plants. Due to their high commercial value, several illegal practices of removal and commercialization have led to the verge of extinction. This study aimed to develop protocols for in vitro conservation under slow growth and acclimatization of species of the subfamily Epidendroideae (Orchidaceae), from the state of Sergipe, Brazil. Two experiments were conducted: the first one tested four concentrations of MS (MURASHIGE and SKOOG, 1962) (25%, 50%, 75%, 100%), three orchid species (Catasetum macrocarpum, Oeceoclades maculata, and Polystachya estrellensis), and two temperatures (18 and 25ºC); the second one tested three combinations of carbon sources and osmotic regulators (20 g L-1 sucrose; 10 g L-1 sucrose + 5 g L-1 de mannitol; 10 g L-1 sucrose + 5 g L-1 sorbitol) and the same three species and two temperatures. For the in vitro conservation of C. macrocarpum, O. maculata, and P. estrellensis over 450 days, the use of 25% of MS salts or 20 g.L-1 sucrose at 25ºC is recommended. C. macrocarpum seedlings were acclimatized using sand + shredded pine bark + worm castings at a ratio of 2:2:1 (w:w:w). The use of sand alone is recommended for the acclimatization of O. maculata. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/38776 10.14393/BJ-v35n2a20198-38776 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/38776 |
identifier_str_mv |
10.14393/BJ-v35n2a20198-38776 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/38776/25781 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 35 No. 2 (2019): Mar./Apr.; 356-366 Bioscience Journal ; v. 35 n. 2 (2019): Mar./Apr.; 356-366 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069078311993344 |