Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro

Detalhes bibliográficos
Autor(a) principal: Gheith, Ibtsam
Data de Publicação: 2017
Outros Autores: El-Mahmoudy, Abubakr
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/33736
Resumo: Punica granatum flower and leaf were extracted with hydromethanol 50% and evaluated for antioxidant activity using phosophomolybdenum, DPPH, reducing power, and -OH-scavenging assays; and for anti-inflammatory activity using HRBC-MS assay. Both extracts exhibited antioxidant activities indicated by DPPH decolorization and -OH-scavenging and increased Ferric reducing and phosphomolybdenum assays. Also, both extracts showed anti-inflammatory activities indicated by red blood cell membrane stabilization and reduced haemolysis-resultant haemoglobin. All results were concentration-dependent; and the leaf extract was more potent than that of the flower. The IC50 values for the flower and leaf extracts were 777.6 ± 1.48 & 656.4 ± 1.79; 184.3 ± 1.803 & 113.9 ± 2.001; 130.8 ± 1.66 & 81.4 ± 2.1; 132.4 ± 1.55 & 79.67 ± 0.03; and 126.1 ± 1.35 & 67.25 ± 1.28 μg/mL in phosophomolybdenum, DPPH, reducing power, -OH-scavenging and red blood cell-membrane stability assays, respectively. Phytochemical analysis revealed presence of tannins and flavonoids with results more obvious in the leaf extract. These active principle contents may account for the recorded antioxidant and anti-inflammatory activities of both extracts. These findings provide evidence for the possible beneficial applications of the Punica granatum leaf and flower extracts in stress-related disease conditions and for maintenance of normal health status and well-being as well.
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spelling Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro Potenciais antioxidantes e anti-inflamatórios potentes de folhas de Punica granatum e extratos hidrometanólicos de flores in vitro Punica granatumExtractAntioxidantAnti-inflammatoryOxidative stress.Punica granatumExtratoAntioxidanteAnti-inflamatórioEstresse oxidativoPunica granatum flower and leaf were extracted with hydromethanol 50% and evaluated for antioxidant activity using phosophomolybdenum, DPPH, reducing power, and -OH-scavenging assays; and for anti-inflammatory activity using HRBC-MS assay. Both extracts exhibited antioxidant activities indicated by DPPH decolorization and -OH-scavenging and increased Ferric reducing and phosphomolybdenum assays. Also, both extracts showed anti-inflammatory activities indicated by red blood cell membrane stabilization and reduced haemolysis-resultant haemoglobin. All results were concentration-dependent; and the leaf extract was more potent than that of the flower. The IC50 values for the flower and leaf extracts were 777.6 ± 1.48 & 656.4 ± 1.79; 184.3 ± 1.803 & 113.9 ± 2.001; 130.8 ± 1.66 & 81.4 ± 2.1; 132.4 ± 1.55 & 79.67 ± 0.03; and 126.1 ± 1.35 & 67.25 ± 1.28 μg/mL in phosophomolybdenum, DPPH, reducing power, -OH-scavenging and red blood cell-membrane stability assays, respectively. Phytochemical analysis revealed presence of tannins and flavonoids with results more obvious in the leaf extract. These active principle contents may account for the recorded antioxidant and anti-inflammatory activities of both extracts. These findings provide evidence for the possible beneficial applications of the Punica granatum leaf and flower extracts in stress-related disease conditions and for maintenance of normal health status and well-being as well.A flor e a folha de Punica granatum foram extraídas com hidrometanol 50% e avaliadas quanto à atividade antioxidante usando ensaios de fosfomolibdênio, DPPH, poder redutor e de eliminação de OH; E para atividade anti-inflamatória utilizando ensaio de HRBC-MS. Ambos os extratos exibiram actividades antioxidantes indicadas por descoloração do DPPH e por eliminação de OH e aumento nos ensaios de redução férrica e fosfomolibdênio. Além disso, ambos os extratos mostraram atividades anti-inflamatórias indicadas pela estabilização da membrana dos glóbulos vermelhos e redução da hemoglobina resultante da hemólise. Todos os resultados foram dependentes da concentração; E o extrato foliar era mais potente que o da flor. Os valores de IC50 para os extractos de flores e folhas foram de 777,6 ± 1,48 & 656,4 ± 1,79; 184,3 ± 1,803 e 113,9 ± 2,001; 130,8 ± 1,66 & 81,4 ± 2,1; 132,4 ± 1,55 & 79,67 ± 0,03; E 126,1 ± 1,35 & 67,25 ± 1,28 µg / mL em ensaios de fosfomolibdênio, DPPH, poder redutor, eliminação de OH e de estabilidade demembrana de glóbulos vermelhos, respectivamente. A análise fitoquímica revelou presença de taninos e flavonóides com resultados mais evidentes no extrato foliar. Estes conteúdos de princípio activo podem explicar as actividades antioxidantes e anti-inflamatórias registadas de ambos os extractos. Estes resultados fornecem evidências para as possíveis aplicações benéficas da folha de Punica granatum e extratos de flores em condições de doença relacionadas ao estresse e para a manutenção do estado de saúde normal e bem-estar também.EDUFU2017-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3373610.14393/BJ-v33n2-33736Bioscience Journal ; Vol. 33 No. 2 (2017): Mar./Apr.Bioscience Journal ; v. 33 n. 2 (2017): Mar./Apr.1981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/33736/20010Gheith, IbtsamEl-Mahmoudy, Abubakrinfo:eu-repo/semantics/openAccess2021-05-31T22:12:06Zoai:ojs.www.seer.ufu.br:article/33736Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2021-05-31T22:12:06Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
Potenciais antioxidantes e anti-inflamatórios potentes de folhas de Punica granatum e extratos hidrometanólicos de flores in vitro
title Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
spellingShingle Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
Gheith, Ibtsam
Punica granatum
Extract
Antioxidant
Anti-inflammatory
Oxidative stress.
Punica granatum
Extrato
Antioxidante
Anti-inflamatório
Estresse oxidativo
title_short Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
title_full Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
title_fullStr Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
title_full_unstemmed Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
title_sort Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro
author Gheith, Ibtsam
author_facet Gheith, Ibtsam
El-Mahmoudy, Abubakr
author_role author
author2 El-Mahmoudy, Abubakr
author2_role author
dc.contributor.author.fl_str_mv Gheith, Ibtsam
El-Mahmoudy, Abubakr
dc.subject.por.fl_str_mv Punica granatum
Extract
Antioxidant
Anti-inflammatory
Oxidative stress.
Punica granatum
Extrato
Antioxidante
Anti-inflamatório
Estresse oxidativo
topic Punica granatum
Extract
Antioxidant
Anti-inflammatory
Oxidative stress.
Punica granatum
Extrato
Antioxidante
Anti-inflamatório
Estresse oxidativo
description Punica granatum flower and leaf were extracted with hydromethanol 50% and evaluated for antioxidant activity using phosophomolybdenum, DPPH, reducing power, and -OH-scavenging assays; and for anti-inflammatory activity using HRBC-MS assay. Both extracts exhibited antioxidant activities indicated by DPPH decolorization and -OH-scavenging and increased Ferric reducing and phosphomolybdenum assays. Also, both extracts showed anti-inflammatory activities indicated by red blood cell membrane stabilization and reduced haemolysis-resultant haemoglobin. All results were concentration-dependent; and the leaf extract was more potent than that of the flower. The IC50 values for the flower and leaf extracts were 777.6 ± 1.48 & 656.4 ± 1.79; 184.3 ± 1.803 & 113.9 ± 2.001; 130.8 ± 1.66 & 81.4 ± 2.1; 132.4 ± 1.55 & 79.67 ± 0.03; and 126.1 ± 1.35 & 67.25 ± 1.28 μg/mL in phosophomolybdenum, DPPH, reducing power, -OH-scavenging and red blood cell-membrane stability assays, respectively. Phytochemical analysis revealed presence of tannins and flavonoids with results more obvious in the leaf extract. These active principle contents may account for the recorded antioxidant and anti-inflammatory activities of both extracts. These findings provide evidence for the possible beneficial applications of the Punica granatum leaf and flower extracts in stress-related disease conditions and for maintenance of normal health status and well-being as well.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/33736
10.14393/BJ-v33n2-33736
url https://seer.ufu.br/index.php/biosciencejournal/article/view/33736
identifier_str_mv 10.14393/BJ-v33n2-33736
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/33736/20010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 33 No. 2 (2017): Mar./Apr.
Bioscience Journal ; v. 33 n. 2 (2017): Mar./Apr.
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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