Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/42403 |
Resumo: | Endoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Concord. Endophytes were grown on a carboxymethylcellulose (CMC) medium and the cup plate assay showed that eight strains (belonging to genera Cochliobolus, Diaporthe, Fusarium and Phoma) had positive results: enzymatic halos ranged from 10.8±0.02 to 15.5±0.07 mm in diameter. Diaporthe sp. strains (GenBank accession codes KM362392, KM362368 and KM362378) and Fusarium culmorum KM362384 were highlighted as the most promising sources. Further, PH and SD as substrates for the fermentation of these fungi were evaluated by the cup plate assay and endoglucanase activity assay. Highest halo diameters were obtained for Diaporthe sp. KM362392: 16.1±0.01 mm (CMC), 14.5±0.01 mm (PH) and 14.7±0.03 mm (SD). The fungus also presented the highest levels of endoglucanase activity: analysis of variance revealed that CMC (3.52±0.98 μmol/min), PH (2.93±0.23 μmol/min) and SD (3.26±0.38 μmol/min) were similarly efficient as substrates. Results deepen knowledge on V. labrusca endophytes that may be endoglucanase sources, even though further optimizations in submerged cultures with PH and SD should be undertaken to increase the enzymatic production from these wastes. |
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Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substratesProdução de endoglucanase por fungos endofíticos isolados de Vitis labrusca L. utilizando casca de amendoim e serragem como substratosCellulaseMicrobial enzymeEndophytesAgro-industrial wastesSubmerged fermentationBiological SciencesEndoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Concord. Endophytes were grown on a carboxymethylcellulose (CMC) medium and the cup plate assay showed that eight strains (belonging to genera Cochliobolus, Diaporthe, Fusarium and Phoma) had positive results: enzymatic halos ranged from 10.8±0.02 to 15.5±0.07 mm in diameter. Diaporthe sp. strains (GenBank accession codes KM362392, KM362368 and KM362378) and Fusarium culmorum KM362384 were highlighted as the most promising sources. Further, PH and SD as substrates for the fermentation of these fungi were evaluated by the cup plate assay and endoglucanase activity assay. Highest halo diameters were obtained for Diaporthe sp. KM362392: 16.1±0.01 mm (CMC), 14.5±0.01 mm (PH) and 14.7±0.03 mm (SD). The fungus also presented the highest levels of endoglucanase activity: analysis of variance revealed that CMC (3.52±0.98 μmol/min), PH (2.93±0.23 μmol/min) and SD (3.26±0.38 μmol/min) were similarly efficient as substrates. Results deepen knowledge on V. labrusca endophytes that may be endoglucanase sources, even though further optimizations in submerged cultures with PH and SD should be undertaken to increase the enzymatic production from these wastes.Endoglucanases são enzimas amplamente empregadas em diferentes setores industriais; embora sua produção apresente custos elevados. Estudos sobre novas fontes microbianas e substratos mais baratos são de grande importância, incluindo os resíduos agroindustriais. Nesse estudo, casca de amendoim (CA) e serragem (SE) foram testadas como substratos para o cultivo submerso de 14 fungos endofíticos isolados das cultivares Bordô e Concord de videira (Vitis labrusca L.) Os endófitos foram crescidos em meio contendo carboximetilcelulose (CMC) e o ensaio cup plate mostrou resultados positivos para oito fungos (pertencentes aos gêneros Cochliobolus, Diaporthe, Fusarium and Phoma); os halos enzimáticos variaram entre 10,8±0,02 e 15,5±0,07 mm de diâmetro. Linhagens de Diaporthe sp. (códigos de acesso no GenBank KM362392, KM362368 e KM362378) e Fusarium culmorum KM362384 se destacaram como produtores mais promissores. Então, o uso de CA e SE como substratos para a fermentação desses fungos foi avaliado pelo ensaio cup plate e pela quantificação da atividade de endoglucanase. Os maiores halos enzimáticos foram obtidos para Diaporthe sp. KM362392: 16,1±0,01 mm (CMC), 14,5±0,01 mm (CA) e 14,7±0,03 mm (SE). Esse fungo também apresentou os maiores níveis de endoglucanase: a análise de variância revelou que CMC (3,52±0,98 μmol/min), CA (2,93±0,23 μmol/min) e SE (3,26±0,38 μmol/min) foram substratos similarmente eficientes. Esses resultados expandem o conhecimento sobre endófitos de V. labrusca que são fontes de endoglucanases; futuras otimizações quanto ao cultivo submerso com CA e SE podem ser utilizadas para aumentar a produção enzimática a partir do uso desses resíduos.EDUFU2019-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4240310.14393/BJ-v35n3a2019-42403Bioscience Journal ; Vol. 35 No. 3 (2019): May/June; 933-940Bioscience Journal ; v. 35 n. 3 (2019): May/June; 933-9401981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42403/26130Brazil; ContemporaryCopyright (c) 2019 Aretusa Cristina Felber, Vânia Specian, Ravely Casarotti Orlandelli, Alessandra Tenório Costa, Julio Cesar Polonio, Káthia Socorro Mathias Mourão, João Alencar Pamphilehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFelber, Aretusa CristinaSpecian, VâniaOrlandelli, Ravely CasarottiCosta, Alessandra TenórioPolonio, Julio CesarMourão, Káthia Socorro MathiasPamphile, João Alencar2022-02-01T16:13:34Zoai:ojs.www.seer.ufu.br:article/42403Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-01T16:13:34Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates Produção de endoglucanase por fungos endofíticos isolados de Vitis labrusca L. utilizando casca de amendoim e serragem como substratos |
title |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates |
spellingShingle |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates Felber, Aretusa Cristina Cellulase Microbial enzyme Endophytes Agro-industrial wastes Submerged fermentation Biological Sciences |
title_short |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates |
title_full |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates |
title_fullStr |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates |
title_full_unstemmed |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates |
title_sort |
Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates |
author |
Felber, Aretusa Cristina |
author_facet |
Felber, Aretusa Cristina Specian, Vânia Orlandelli, Ravely Casarotti Costa, Alessandra Tenório Polonio, Julio Cesar Mourão, Káthia Socorro Mathias Pamphile, João Alencar |
author_role |
author |
author2 |
Specian, Vânia Orlandelli, Ravely Casarotti Costa, Alessandra Tenório Polonio, Julio Cesar Mourão, Káthia Socorro Mathias Pamphile, João Alencar |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Felber, Aretusa Cristina Specian, Vânia Orlandelli, Ravely Casarotti Costa, Alessandra Tenório Polonio, Julio Cesar Mourão, Káthia Socorro Mathias Pamphile, João Alencar |
dc.subject.por.fl_str_mv |
Cellulase Microbial enzyme Endophytes Agro-industrial wastes Submerged fermentation Biological Sciences |
topic |
Cellulase Microbial enzyme Endophytes Agro-industrial wastes Submerged fermentation Biological Sciences |
description |
Endoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Concord. Endophytes were grown on a carboxymethylcellulose (CMC) medium and the cup plate assay showed that eight strains (belonging to genera Cochliobolus, Diaporthe, Fusarium and Phoma) had positive results: enzymatic halos ranged from 10.8±0.02 to 15.5±0.07 mm in diameter. Diaporthe sp. strains (GenBank accession codes KM362392, KM362368 and KM362378) and Fusarium culmorum KM362384 were highlighted as the most promising sources. Further, PH and SD as substrates for the fermentation of these fungi were evaluated by the cup plate assay and endoglucanase activity assay. Highest halo diameters were obtained for Diaporthe sp. KM362392: 16.1±0.01 mm (CMC), 14.5±0.01 mm (PH) and 14.7±0.03 mm (SD). The fungus also presented the highest levels of endoglucanase activity: analysis of variance revealed that CMC (3.52±0.98 μmol/min), PH (2.93±0.23 μmol/min) and SD (3.26±0.38 μmol/min) were similarly efficient as substrates. Results deepen knowledge on V. labrusca endophytes that may be endoglucanase sources, even though further optimizations in submerged cultures with PH and SD should be undertaken to increase the enzymatic production from these wastes. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42403 10.14393/BJ-v35n3a2019-42403 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42403 |
identifier_str_mv |
10.14393/BJ-v35n3a2019-42403 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42403/26130 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 35 No. 3 (2019): May/June; 933-940 Bioscience Journal ; v. 35 n. 3 (2019): May/June; 933-940 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069080402853888 |