Lentinus crinitus response to blue light on carbohydrate-active enzymes
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/49986 |
Resumo: | Fungi are capable of sensing light from ultraviolet to far-red and they use light as a source of information about the environment anticipating stress and adverse conditions. Lentinus crinitus is a lignin-degrading fungus which produces laccase and other enzymes of biotechnological interest. The effect of blue light on fungal enzymatic activity has been studied; however, it has not been found studies on the effect of the blue light on carbohydrate-active enzymes and on mycelial biomass production of L. crinitus. We aimed to investigate carbohydrate-active enzymes activity and mycelial biomass production of L. crinitus cultivated under continuous illumination with blue light. L. crinitus was cultivated in malt extract medium in the dark, without agitation, and under continuous illumination with blue light-emitting diodes. The blue light reduced the total cellulase, pectinase and xylanase activities but increased the endoglucanase activity. Blue light reduced the mycelial growth of L. crinitus in 26% and the enzymatic activity-to-mycelial biomass ratio (U mg-1 dry basis) increased in 10% total cellulase, 33% endoglucanase, and 16% pectinase activities. Also, it is suggested that L. crinitus has a photosensory system and it could lead to new process of obtaining enzymes of biotechnological interest. |
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Lentinus crinitus response to blue light on carbohydrate-active enzymesEfeito da luz azul na produção de enzimas ativas sobre carboidratos de Lentinus crinitusCellulase.Hemicellulase.Lentinus crinitus.Polygalacturonase.Visible light wavelengthsWhite-rot fungi.Agricultural SciencesCelulase.Hemicelulase.Lentinus crinitus.Poligalacturonase.Luz visível.Fungo da podridão branca.Fungi are capable of sensing light from ultraviolet to far-red and they use light as a source of information about the environment anticipating stress and adverse conditions. Lentinus crinitus is a lignin-degrading fungus which produces laccase and other enzymes of biotechnological interest. The effect of blue light on fungal enzymatic activity has been studied; however, it has not been found studies on the effect of the blue light on carbohydrate-active enzymes and on mycelial biomass production of L. crinitus. We aimed to investigate carbohydrate-active enzymes activity and mycelial biomass production of L. crinitus cultivated under continuous illumination with blue light. L. crinitus was cultivated in malt extract medium in the dark, without agitation, and under continuous illumination with blue light-emitting diodes. The blue light reduced the total cellulase, pectinase and xylanase activities but increased the endoglucanase activity. Blue light reduced the mycelial growth of L. crinitus in 26% and the enzymatic activity-to-mycelial biomass ratio (U mg-1 dry basis) increased in 10% total cellulase, 33% endoglucanase, and 16% pectinase activities. Also, it is suggested that L. crinitus has a photosensory system and it could lead to new process of obtaining enzymes of biotechnological interest.Fungos são capazes de sentir a luz com comprimentos de onda que variam do ultravioleta ao infravermelho e usam a luz como fonte de informação sobre o ambiente, antecipando condições adversas e de estresse. Lentinus crinitus é um fungo ligninolítico que produz lacase e outras enzimas de interesse biotecnológico. O efeito da luz azul na atividade enzimática de fungos já foi estudado, contudo, ainda não há estudos sobre o efeito da luz azul na produção de enzimas ativas sobre carboidratos (CAZymes, carbohydrate-active enzymes) e de biomassa micelial de L. crinitus. O objetivo deste estudo foi investigar a avitivade de CAZymes e a produção de biomassa micelial de L. crinitus cultivado sob iluminação continua com luz azul. L. crinitus foi cultivado em meio extrato de malte, sem agitação, na ausência de luz e sob luz continua fornecida por diodos emissores de luz azul. A luz azul reduziu a atividade de cellulase total, pectinase e xilanase, mas aumentou a atividade de endoglucanase. A luz azul reduziu o crescimento micelial de L. crinitus em 26% e aumentou a razão atividade enzimática/biomassa micelial (U mg-1 em base seca) de cellulase total em 10%, endoglucanase em 33% e pectinase em 16%. Além disso, sugere-se que L. crinitus possua um sistema fotossensorial que poderia ser explorado para a otimização de bioprocessos que visam a obtenção de enzimas de interesse biotecnológico.EDUFU2020-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4998610.14393/BJ-v36n3a2020-49986Bioscience Journal ; Vol. 36 No. 3 (2020): May/June; 924-931Bioscience Journal ; v. 36 n. 3 (2020): Maio/Junho; 924-9311981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/49986/28635Brazil; ContemporaryCopyright (c) 2020 Renan Alberto Marim, Katielle Vieira Avelino, Marisangela Isabel Wietzikoski Halabura, Nelma Lopes Araújo, Thiago Teodoro Santana, Giani Andrea Linde, Nelson Barros Colauto, Juliana Silveira do Vallehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAlberto Marim, RenanVieira Avelino, KatielleWietzikoski Halabura, Marisangela IsabelLopes Araújo, NelmaTeodoro Santana, ThiagoLinde, Giani AndreaBarros Colauto, NelsonSilveira do Valle, Juliana2022-06-20T13:04:21Zoai:ojs.www.seer.ufu.br:article/49986Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-06-20T13:04:21Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Lentinus crinitus response to blue light on carbohydrate-active enzymes Efeito da luz azul na produção de enzimas ativas sobre carboidratos de Lentinus crinitus |
title |
Lentinus crinitus response to blue light on carbohydrate-active enzymes |
spellingShingle |
Lentinus crinitus response to blue light on carbohydrate-active enzymes Alberto Marim, Renan Cellulase. Hemicellulase. Lentinus crinitus. Polygalacturonase. Visible light wavelengths White-rot fungi. Agricultural Sciences Celulase. Hemicelulase. Lentinus crinitus. Poligalacturonase. Luz visível. Fungo da podridão branca. |
title_short |
Lentinus crinitus response to blue light on carbohydrate-active enzymes |
title_full |
Lentinus crinitus response to blue light on carbohydrate-active enzymes |
title_fullStr |
Lentinus crinitus response to blue light on carbohydrate-active enzymes |
title_full_unstemmed |
Lentinus crinitus response to blue light on carbohydrate-active enzymes |
title_sort |
Lentinus crinitus response to blue light on carbohydrate-active enzymes |
author |
Alberto Marim, Renan |
author_facet |
Alberto Marim, Renan Vieira Avelino, Katielle Wietzikoski Halabura, Marisangela Isabel Lopes Araújo, Nelma Teodoro Santana, Thiago Linde, Giani Andrea Barros Colauto, Nelson Silveira do Valle, Juliana |
author_role |
author |
author2 |
Vieira Avelino, Katielle Wietzikoski Halabura, Marisangela Isabel Lopes Araújo, Nelma Teodoro Santana, Thiago Linde, Giani Andrea Barros Colauto, Nelson Silveira do Valle, Juliana |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Alberto Marim, Renan Vieira Avelino, Katielle Wietzikoski Halabura, Marisangela Isabel Lopes Araújo, Nelma Teodoro Santana, Thiago Linde, Giani Andrea Barros Colauto, Nelson Silveira do Valle, Juliana |
dc.subject.por.fl_str_mv |
Cellulase. Hemicellulase. Lentinus crinitus. Polygalacturonase. Visible light wavelengths White-rot fungi. Agricultural Sciences Celulase. Hemicelulase. Lentinus crinitus. Poligalacturonase. Luz visível. Fungo da podridão branca. |
topic |
Cellulase. Hemicellulase. Lentinus crinitus. Polygalacturonase. Visible light wavelengths White-rot fungi. Agricultural Sciences Celulase. Hemicelulase. Lentinus crinitus. Poligalacturonase. Luz visível. Fungo da podridão branca. |
description |
Fungi are capable of sensing light from ultraviolet to far-red and they use light as a source of information about the environment anticipating stress and adverse conditions. Lentinus crinitus is a lignin-degrading fungus which produces laccase and other enzymes of biotechnological interest. The effect of blue light on fungal enzymatic activity has been studied; however, it has not been found studies on the effect of the blue light on carbohydrate-active enzymes and on mycelial biomass production of L. crinitus. We aimed to investigate carbohydrate-active enzymes activity and mycelial biomass production of L. crinitus cultivated under continuous illumination with blue light. L. crinitus was cultivated in malt extract medium in the dark, without agitation, and under continuous illumination with blue light-emitting diodes. The blue light reduced the total cellulase, pectinase and xylanase activities but increased the endoglucanase activity. Blue light reduced the mycelial growth of L. crinitus in 26% and the enzymatic activity-to-mycelial biomass ratio (U mg-1 dry basis) increased in 10% total cellulase, 33% endoglucanase, and 16% pectinase activities. Also, it is suggested that L. crinitus has a photosensory system and it could lead to new process of obtaining enzymes of biotechnological interest. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/49986 10.14393/BJ-v36n3a2020-49986 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/49986 |
identifier_str_mv |
10.14393/BJ-v36n3a2020-49986 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/49986/28635 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 36 No. 3 (2020): May/June; 924-931 Bioscience Journal ; v. 36 n. 3 (2020): Maio/Junho; 924-931 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069082321747968 |