RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/49905 |
Resumo: | In this study, the recombinant gut protein rRa92A produced in Pichia pastoris yeast cells was used to immunize cattle in two experiments, one in Brazil and the other in Uganda. In both experiments, the animals were intramuscularly (IM) injected with 200 µg of recombinant protein in Brazil on days 0, 30 and 51 and in Uganda on days 0, 30. Blood samples for sera separation were collected from different days in both experiments. These samples were analyzed by ELISAs. In Brazil, ticks collected from the animals during the experimental period were analyzed for biological parameters. At Uganda, blood was collected to assess blood parameters, clinical signs were recorded and adult tick (Rhipicephalus appendiculatus) counts were performed. All animals of the vaccinated groups were shown to produce antibodies, and it was not possible to detect an effect on Rhipicephalus microplus. All the clinical parameters were considered within the normal ranges for both the experimental and control groups in Uganda. Antibody absorbance was elevated after each immunization and remained high until the end of the experiments, remaining low in the control animals. The results of stall test carried out in Brazil using R. microplus tick showed efficacy of 21.95%. The rRa92A immunization trial experiments in Uganda showing a decrease of 55.2% in the number of engorged adult ticks, which was statistically significant (p<0.05). Assessment of the immunogenicity of Ra92A produced in the P. pastoris expression system in bovines is reported for the first time, and the protein acted as a concealed antigen. |
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RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and UgandaImmunogenicityRa92A proteinRhipicephalus appendiculatusTicksVaccine.Agricultural SciencesIn this study, the recombinant gut protein rRa92A produced in Pichia pastoris yeast cells was used to immunize cattle in two experiments, one in Brazil and the other in Uganda. In both experiments, the animals were intramuscularly (IM) injected with 200 µg of recombinant protein in Brazil on days 0, 30 and 51 and in Uganda on days 0, 30. Blood samples for sera separation were collected from different days in both experiments. These samples were analyzed by ELISAs. In Brazil, ticks collected from the animals during the experimental period were analyzed for biological parameters. At Uganda, blood was collected to assess blood parameters, clinical signs were recorded and adult tick (Rhipicephalus appendiculatus) counts were performed. All animals of the vaccinated groups were shown to produce antibodies, and it was not possible to detect an effect on Rhipicephalus microplus. All the clinical parameters were considered within the normal ranges for both the experimental and control groups in Uganda. Antibody absorbance was elevated after each immunization and remained high until the end of the experiments, remaining low in the control animals. The results of stall test carried out in Brazil using R. microplus tick showed efficacy of 21.95%. The rRa92A immunization trial experiments in Uganda showing a decrease of 55.2% in the number of engorged adult ticks, which was statistically significant (p<0.05). Assessment of the immunogenicity of Ra92A produced in the P. pastoris expression system in bovines is reported for the first time, and the protein acted as a concealed antigen.EDUFU2021-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4990510.14393/BJ-v37n0a2021-49905Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37068Bioscience Journal ; v. 37 (2021): Continuous Publication; e370681981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/49905/32808Brazil; ContemporaryCopyright (c) 2021 Rodrigo Casquero Cunha, Margaret Saimo-Kahwa, Marcos Valério Garcia, Francisco Denis Souza Santos, Emukule Samuel, Nanteza Ann, Kokas Ikwap, Claire Julie Akwongo, Fábio Pereira Leivas Leite, Renato Andreottihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCunha, Rodrigo Casquero Saimo-Kahwa, Margaret Valério Garcia, MarcosSantos, Francisco Denis Souza Samuel, Emukule Ann, Nanteza Ikwap, Kokas Julie Akwongo, Claire Leivas Leite, Fábio PereiraAndreotti, Renato2022-05-25T13:04:25Zoai:ojs.www.seer.ufu.br:article/49905Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-25T13:04:25Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
title |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
spellingShingle |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda Cunha, Rodrigo Casquero Immunogenicity Ra92A protein Rhipicephalus appendiculatus Ticks Vaccine. Agricultural Sciences |
title_short |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
title_full |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
title_fullStr |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
title_full_unstemmed |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
title_sort |
RA92A recombinant protein as immunogen to protect cattle against tick challenge in Brazil and Uganda |
author |
Cunha, Rodrigo Casquero |
author_facet |
Cunha, Rodrigo Casquero Saimo-Kahwa, Margaret Valério Garcia, Marcos Santos, Francisco Denis Souza Samuel, Emukule Ann, Nanteza Ikwap, Kokas Julie Akwongo, Claire Leivas Leite, Fábio Pereira Andreotti, Renato |
author_role |
author |
author2 |
Saimo-Kahwa, Margaret Valério Garcia, Marcos Santos, Francisco Denis Souza Samuel, Emukule Ann, Nanteza Ikwap, Kokas Julie Akwongo, Claire Leivas Leite, Fábio Pereira Andreotti, Renato |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Rodrigo Casquero Saimo-Kahwa, Margaret Valério Garcia, Marcos Santos, Francisco Denis Souza Samuel, Emukule Ann, Nanteza Ikwap, Kokas Julie Akwongo, Claire Leivas Leite, Fábio Pereira Andreotti, Renato |
dc.subject.por.fl_str_mv |
Immunogenicity Ra92A protein Rhipicephalus appendiculatus Ticks Vaccine. Agricultural Sciences |
topic |
Immunogenicity Ra92A protein Rhipicephalus appendiculatus Ticks Vaccine. Agricultural Sciences |
description |
In this study, the recombinant gut protein rRa92A produced in Pichia pastoris yeast cells was used to immunize cattle in two experiments, one in Brazil and the other in Uganda. In both experiments, the animals were intramuscularly (IM) injected with 200 µg of recombinant protein in Brazil on days 0, 30 and 51 and in Uganda on days 0, 30. Blood samples for sera separation were collected from different days in both experiments. These samples were analyzed by ELISAs. In Brazil, ticks collected from the animals during the experimental period were analyzed for biological parameters. At Uganda, blood was collected to assess blood parameters, clinical signs were recorded and adult tick (Rhipicephalus appendiculatus) counts were performed. All animals of the vaccinated groups were shown to produce antibodies, and it was not possible to detect an effect on Rhipicephalus microplus. All the clinical parameters were considered within the normal ranges for both the experimental and control groups in Uganda. Antibody absorbance was elevated after each immunization and remained high until the end of the experiments, remaining low in the control animals. The results of stall test carried out in Brazil using R. microplus tick showed efficacy of 21.95%. The rRa92A immunization trial experiments in Uganda showing a decrease of 55.2% in the number of engorged adult ticks, which was statistically significant (p<0.05). Assessment of the immunogenicity of Ra92A produced in the P. pastoris expression system in bovines is reported for the first time, and the protein acted as a concealed antigen. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/49905 10.14393/BJ-v37n0a2021-49905 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/49905 |
identifier_str_mv |
10.14393/BJ-v37n0a2021-49905 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/49905/32808 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37068 Bioscience Journal ; v. 37 (2021): Continuous Publication; e37068 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069082299727872 |