Molecular characterization and core collection evaluation of Manihot esculenta Crantz

Detalhes bibliográficos
Autor(a) principal: Costa, Nathalia Almeida
Data de Publicação: 2020
Outros Autores: Azêvedo, Hellen Sandra Freires da Silva, Silva, Luciélio Manoel da, Cunha, Elisa Ferreira Moura, Siviero, Amauri, Campos, Tatiana de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/48247
Resumo: Cassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908.  Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn’t differentiate bitter from sweet cassava, and there wasn’t correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections.
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spelling Molecular characterization and core collection evaluation of Manihot esculenta CrantzCassavaCore collectionGenetic resourcesManihot esculentaMicrosatellite markersVariabilityCassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908.  Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn’t differentiate bitter from sweet cassava, and there wasn’t correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections.EDUFU2020-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4824710.14393/BJ-v36n0a2020-48247Bioscience Journal ; Vol. 36 (2020): Supplement1; 22-35Bioscience Journal ; v. 36 (2020): Supplement1; 22-351981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/48247/31075Copyright (c) 2020 Nathalia Almeida Costa, Hellen Sandra Freires da Silva Azêvedo, Luciélio Manoel da Silva, Elisa Ferreira Moura Cunha, Amauri Siviero, Tatiana de Camposhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta, Nathalia AlmeidaAzêvedo, Hellen Sandra Freires da SilvaSilva, Luciélio Manoel da Cunha, Elisa Ferreira MouraSiviero, Amauri Campos, Tatiana de2022-05-25T18:36:01Zoai:ojs.www.seer.ufu.br:article/48247Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-25T18:36:01Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Molecular characterization and core collection evaluation of Manihot esculenta Crantz
title Molecular characterization and core collection evaluation of Manihot esculenta Crantz
spellingShingle Molecular characterization and core collection evaluation of Manihot esculenta Crantz
Costa, Nathalia Almeida
Cassava
Core collection
Genetic resources
Manihot esculenta
Microsatellite markers
Variability
title_short Molecular characterization and core collection evaluation of Manihot esculenta Crantz
title_full Molecular characterization and core collection evaluation of Manihot esculenta Crantz
title_fullStr Molecular characterization and core collection evaluation of Manihot esculenta Crantz
title_full_unstemmed Molecular characterization and core collection evaluation of Manihot esculenta Crantz
title_sort Molecular characterization and core collection evaluation of Manihot esculenta Crantz
author Costa, Nathalia Almeida
author_facet Costa, Nathalia Almeida
Azêvedo, Hellen Sandra Freires da Silva
Silva, Luciélio Manoel da
Cunha, Elisa Ferreira Moura
Siviero, Amauri
Campos, Tatiana de
author_role author
author2 Azêvedo, Hellen Sandra Freires da Silva
Silva, Luciélio Manoel da
Cunha, Elisa Ferreira Moura
Siviero, Amauri
Campos, Tatiana de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Nathalia Almeida
Azêvedo, Hellen Sandra Freires da Silva
Silva, Luciélio Manoel da
Cunha, Elisa Ferreira Moura
Siviero, Amauri
Campos, Tatiana de
dc.subject.por.fl_str_mv Cassava
Core collection
Genetic resources
Manihot esculenta
Microsatellite markers
Variability
topic Cassava
Core collection
Genetic resources
Manihot esculenta
Microsatellite markers
Variability
description Cassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908.  Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn’t differentiate bitter from sweet cassava, and there wasn’t correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/48247
10.14393/BJ-v36n0a2020-48247
url https://seer.ufu.br/index.php/biosciencejournal/article/view/48247
identifier_str_mv 10.14393/BJ-v36n0a2020-48247
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/48247/31075
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 36 (2020): Supplement1; 22-35
Bioscience Journal ; v. 36 (2020): Supplement1; 22-35
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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