Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/50347 |
Resumo: | Breast cancer appears as the main gynecological cancer and presents high morbidity and mortality. Because most diagnoses are made belatedly, it is necessary to seek therapeutic options that aim for advanced stages of the disease. This study aims to evaluate the antitumoral action of Stryphnodendron adstringens fruit extracts on 4T1 murine mammary carcinoma cell culture. The inhibitory potential of S. adstringens fruit extract on the metalloproteinases (MMPs) 2 and 9 was evaluated through zymography. From these results, MTT assays were performed to evaluate the extracts’ effects on the murine mammary carcinoma 4T1 line cell viability. From the crude extract, the following extracts were obtained: hydroalcoholic (SAFCEA), hexane (SAFCEB), chloroform (SAFCEC), and ethyl acetate (SAFCED). Lastly, the migration of the cells treated with extracts SAFCEA and SAFCED was verified. The hydroalcoholic extract (SAFCEA) was the most efficient in inhibiting gelatinases. During the phytochemical study, it was noted that alkaloids were present in all partitions. The 50 % growth inhibition (IC50) concentrations found were: 40.1 μg/mL (SAFCEA) and 70.14 μg/mL (SAFCED). After the cellular cytotoxicity assay, cell morphology was altered by treatment with the selected partitions (SAFCEA and SAFCED), obtaining morphology consistent with apoptosis. The results demonstrate that S. adstringens extracts exhibit the inhibitory activity of MMP-2 and MMP-9 as well as cytotoxicity toward 4T1 tumor cells. These findings indicate that follow-up studies of the partitions from S. adstringens may lead to the development of novel chemotherapeutics for oncological treatments. |
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Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor lineCarcinomaCell CultureCerrado PlantsCytotoxicityStryphnodendron adstringens.Biological SciencesBreast cancer appears as the main gynecological cancer and presents high morbidity and mortality. Because most diagnoses are made belatedly, it is necessary to seek therapeutic options that aim for advanced stages of the disease. This study aims to evaluate the antitumoral action of Stryphnodendron adstringens fruit extracts on 4T1 murine mammary carcinoma cell culture. The inhibitory potential of S. adstringens fruit extract on the metalloproteinases (MMPs) 2 and 9 was evaluated through zymography. From these results, MTT assays were performed to evaluate the extracts’ effects on the murine mammary carcinoma 4T1 line cell viability. From the crude extract, the following extracts were obtained: hydroalcoholic (SAFCEA), hexane (SAFCEB), chloroform (SAFCEC), and ethyl acetate (SAFCED). Lastly, the migration of the cells treated with extracts SAFCEA and SAFCED was verified. The hydroalcoholic extract (SAFCEA) was the most efficient in inhibiting gelatinases. During the phytochemical study, it was noted that alkaloids were present in all partitions. The 50 % growth inhibition (IC50) concentrations found were: 40.1 μg/mL (SAFCEA) and 70.14 μg/mL (SAFCED). After the cellular cytotoxicity assay, cell morphology was altered by treatment with the selected partitions (SAFCEA and SAFCED), obtaining morphology consistent with apoptosis. The results demonstrate that S. adstringens extracts exhibit the inhibitory activity of MMP-2 and MMP-9 as well as cytotoxicity toward 4T1 tumor cells. These findings indicate that follow-up studies of the partitions from S. adstringens may lead to the development of novel chemotherapeutics for oncological treatments.EDUFU2021-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5034710.14393/BJ-v37n0a2021-50347Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37055Bioscience Journal ; v. 37 (2021): Continuous Publication; e370551981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/50347/32682Brazil; ContemporaryCopyright (c) 2021 Alessandra Aparecida de Melo Souza, Elias Raad Gervásio, Thais Barbosa de Paula, Luis Ribeiro da Silva Neto, Fernanda Pinheiro Chagas Fernandes, Gabriel Furtado Leite, Filipe Peres Barreto , Rosy Iara Maciel de Azambuja Ribeiro https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSouza, Alessandra Aparecida de Melo Gervásio, Elias Raad de Paula, Thais Barbosa da Silva Neto, Luis RibeiroFernandes, Fernanda Pinheiro Chagas Leite, Gabriel Furtado Barreto , Filipe Peres Ribeiro , Rosy Iara Maciel de Azambuja2022-05-25T16:56:07Zoai:ojs.www.seer.ufu.br:article/50347Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-25T16:56:07Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
title |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
spellingShingle |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line Souza, Alessandra Aparecida de Melo Carcinoma Cell Culture Cerrado Plants Cytotoxicity Stryphnodendron adstringens. Biological Sciences |
title_short |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
title_full |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
title_fullStr |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
title_full_unstemmed |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
title_sort |
Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line |
author |
Souza, Alessandra Aparecida de Melo |
author_facet |
Souza, Alessandra Aparecida de Melo Gervásio, Elias Raad de Paula, Thais Barbosa da Silva Neto, Luis Ribeiro Fernandes, Fernanda Pinheiro Chagas Leite, Gabriel Furtado Barreto , Filipe Peres Ribeiro , Rosy Iara Maciel de Azambuja |
author_role |
author |
author2 |
Gervásio, Elias Raad de Paula, Thais Barbosa da Silva Neto, Luis Ribeiro Fernandes, Fernanda Pinheiro Chagas Leite, Gabriel Furtado Barreto , Filipe Peres Ribeiro , Rosy Iara Maciel de Azambuja |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Alessandra Aparecida de Melo Gervásio, Elias Raad de Paula, Thais Barbosa da Silva Neto, Luis Ribeiro Fernandes, Fernanda Pinheiro Chagas Leite, Gabriel Furtado Barreto , Filipe Peres Ribeiro , Rosy Iara Maciel de Azambuja |
dc.subject.por.fl_str_mv |
Carcinoma Cell Culture Cerrado Plants Cytotoxicity Stryphnodendron adstringens. Biological Sciences |
topic |
Carcinoma Cell Culture Cerrado Plants Cytotoxicity Stryphnodendron adstringens. Biological Sciences |
description |
Breast cancer appears as the main gynecological cancer and presents high morbidity and mortality. Because most diagnoses are made belatedly, it is necessary to seek therapeutic options that aim for advanced stages of the disease. This study aims to evaluate the antitumoral action of Stryphnodendron adstringens fruit extracts on 4T1 murine mammary carcinoma cell culture. The inhibitory potential of S. adstringens fruit extract on the metalloproteinases (MMPs) 2 and 9 was evaluated through zymography. From these results, MTT assays were performed to evaluate the extracts’ effects on the murine mammary carcinoma 4T1 line cell viability. From the crude extract, the following extracts were obtained: hydroalcoholic (SAFCEA), hexane (SAFCEB), chloroform (SAFCEC), and ethyl acetate (SAFCED). Lastly, the migration of the cells treated with extracts SAFCEA and SAFCED was verified. The hydroalcoholic extract (SAFCEA) was the most efficient in inhibiting gelatinases. During the phytochemical study, it was noted that alkaloids were present in all partitions. The 50 % growth inhibition (IC50) concentrations found were: 40.1 μg/mL (SAFCEA) and 70.14 μg/mL (SAFCED). After the cellular cytotoxicity assay, cell morphology was altered by treatment with the selected partitions (SAFCEA and SAFCED), obtaining morphology consistent with apoptosis. The results demonstrate that S. adstringens extracts exhibit the inhibitory activity of MMP-2 and MMP-9 as well as cytotoxicity toward 4T1 tumor cells. These findings indicate that follow-up studies of the partitions from S. adstringens may lead to the development of novel chemotherapeutics for oncological treatments. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/50347 10.14393/BJ-v37n0a2021-50347 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/50347 |
identifier_str_mv |
10.14393/BJ-v37n0a2021-50347 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/50347/32682 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37055 Bioscience Journal ; v. 37 (2021): Continuous Publication; e37055 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069082331185152 |