Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/47738 |
Resumo: | The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose. |
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Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulataEfeito da luz e sacarose na micropropagação fotoautotrófica e fotomixotrófica de Physalis angulataApplied botanyEcological anatomySmall fruit cropsPlant tissue culturePhotopigmentsAgrarianBotânica aplicadaAnatomia ecológicaPequenas frutasCultura de tecidos vegetaisFotopigmentosThe biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose. O interesse biotecnológico em Physalis aumentou nas últimas décadas, porém, ainda existem poucos trabalhos de micropropagação desse gênero. Objetivou-se avaliar sua micropropagação fotoautotrófica e fotomixotrófica com troca gasosa sob sete tipos de iluminação e cinco concentrações de sacarose. Foram utilizados LEDs amarelo, azul, branco, vermelho, verde, vermelho + azul e luz natural filtrada por malha. As concentrações de sacarose foram 0, 7,5, 15, 22,5 e 30 g.L-1. Características fitotécnicas, anatômicas e teor de fotopigmentos foram avaliados através de comprimento de segmento de caule e raíz, número de folhas, área foliar, teores de clorofilas a e b, carotenoides, epiderme adaxial, parênquimas paliçádico e esponjoso e epiderme abaxial. Os dados foram comparados por teste de média Scott-Knott e análise de componentes principais utilizando-se o software R. Comparando-se as variáveis dentro de tipos de iluminação, observou-se que apenas o tratamento screen, iluminação natural filtrada por malha, obteve avaliação máxima em todas as variáveis. Comparando-se os níveis de sucrose, observou-se que o tratamento 15 g.L-1 sacarose obteve o maior número de médias com avaliação máxima. Concluiu-se que a luz natural filtrada por tela com 50% de sombreamento permitiu a micropropagação fotoautotrófica de P. angulata. Observou-se melhores resultados de desenvolvimento na micropropagação fotomixotrófica com 15 g.L-1 de sacarose. EDUFU2020-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4773810.14393/BJ-v36n4a2020-47738Bioscience Journal ; Vol. 36 No. 4 (2020): July/Aug.; 1353-1367Bioscience Journal ; v. 36 n. 4 (2020): julho/agosto; 1353-13671981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/47738/28973Brazil; Contemporary Copyright (c) 2020 Gustavo C Santos, Felipe P Cardoso, Adalvan D Martins, Moacir Pasqual, Paulo C Ossani, José Magno Queiroz, Renata A L S Rezende, Joyce Dória https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Gustavo CCardoso, Felipe PMartins, Adalvan DPasqual, MoacirOssani, Paulo CQueiroz, José Magno Rezende, Renata A L SDória , Joyce 2022-06-14T13:34:10Zoai:ojs.www.seer.ufu.br:article/47738Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-06-14T13:34:10Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata Efeito da luz e sacarose na micropropagação fotoautotrófica e fotomixotrófica de Physalis angulata |
title |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata |
spellingShingle |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata Santos, Gustavo C Applied botany Ecological anatomy Small fruit crops Plant tissue culture Photopigments Agrarian Botânica aplicada Anatomia ecológica Pequenas frutas Cultura de tecidos vegetais Fotopigmentos |
title_short |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata |
title_full |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata |
title_fullStr |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata |
title_full_unstemmed |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata |
title_sort |
Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata |
author |
Santos, Gustavo C |
author_facet |
Santos, Gustavo C Cardoso, Felipe P Martins, Adalvan D Pasqual, Moacir Ossani, Paulo C Queiroz, José Magno Rezende, Renata A L S Dória , Joyce |
author_role |
author |
author2 |
Cardoso, Felipe P Martins, Adalvan D Pasqual, Moacir Ossani, Paulo C Queiroz, José Magno Rezende, Renata A L S Dória , Joyce |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Gustavo C Cardoso, Felipe P Martins, Adalvan D Pasqual, Moacir Ossani, Paulo C Queiroz, José Magno Rezende, Renata A L S Dória , Joyce |
dc.subject.por.fl_str_mv |
Applied botany Ecological anatomy Small fruit crops Plant tissue culture Photopigments Agrarian Botânica aplicada Anatomia ecológica Pequenas frutas Cultura de tecidos vegetais Fotopigmentos |
topic |
Applied botany Ecological anatomy Small fruit crops Plant tissue culture Photopigments Agrarian Botânica aplicada Anatomia ecológica Pequenas frutas Cultura de tecidos vegetais Fotopigmentos |
description |
The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/47738 10.14393/BJ-v36n4a2020-47738 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/47738 |
identifier_str_mv |
10.14393/BJ-v36n4a2020-47738 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/47738/28973 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 36 No. 4 (2020): July/Aug.; 1353-1367 Bioscience Journal ; v. 36 n. 4 (2020): julho/agosto; 1353-1367 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069081108545536 |