Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/42339 |
Resumo: | A sensitive and reliable process was established using high-performance liquid chromatography (HPLC) to distinguish conventional varieties of glyphosate-resistant genetically modified crops via shikimate detection in soybean (Glycine max L. Merril) seeds. Glyphosate has a well-defined mechanism of action. It is the only herbicide that specifically inhibits 5-enolpiruvilshikimate-3-phosphate synthase (EPSPS E.C. 2.5.1.19), which catalyzes the condensation of shikimate with phosphoenolpyruvate. This study is based on the concept that shikimate significantly accumulates in soybean plant tissues after EPSPS inhibition by glyphosate. In plants not subjected to glyphosate, shikimate is not easily detected because it quickly metabolizes into shikimate 3-phosphate and subsequently into 5-enolpiruvilshikimate 3-phosphate through the action of EPSPS. Conversely, in non-genetically modified plants subjected to glyphosate, shikimate metabolism is impaired, resulting in its accumulation. This metabolite can be detected in extremely low quantities (in the microgram range), through HPLC. In this study, six different contrasts were analyzed, each being formed by a transgenic cultivation and its parental strain, subject or not subject to the treatment of soaking with a 0.6% glyphosate solution. Chromatographic analyses indicated shikimate accumulation only in conventional cultivars with seeds previously soaked in a 0.6% glyphosate solution. Thus, this shikimate detection method can be used as a rapid and accurate means to distinguish soybeans with glyphosate-resistant qualities. |
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Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybeanDeterminação cromatográfica de chiquimato para identificação de soja convenciolnal e resistente ao glifosatoGlycine max L. Merril.TransgenesisRR soyHPLCAgricultural SciencesA sensitive and reliable process was established using high-performance liquid chromatography (HPLC) to distinguish conventional varieties of glyphosate-resistant genetically modified crops via shikimate detection in soybean (Glycine max L. Merril) seeds. Glyphosate has a well-defined mechanism of action. It is the only herbicide that specifically inhibits 5-enolpiruvilshikimate-3-phosphate synthase (EPSPS E.C. 2.5.1.19), which catalyzes the condensation of shikimate with phosphoenolpyruvate. This study is based on the concept that shikimate significantly accumulates in soybean plant tissues after EPSPS inhibition by glyphosate. In plants not subjected to glyphosate, shikimate is not easily detected because it quickly metabolizes into shikimate 3-phosphate and subsequently into 5-enolpiruvilshikimate 3-phosphate through the action of EPSPS. Conversely, in non-genetically modified plants subjected to glyphosate, shikimate metabolism is impaired, resulting in its accumulation. This metabolite can be detected in extremely low quantities (in the microgram range), through HPLC. In this study, six different contrasts were analyzed, each being formed by a transgenic cultivation and its parental strain, subject or not subject to the treatment of soaking with a 0.6% glyphosate solution. Chromatographic analyses indicated shikimate accumulation only in conventional cultivars with seeds previously soaked in a 0.6% glyphosate solution. Thus, this shikimate detection method can be used as a rapid and accurate means to distinguish soybeans with glyphosate-resistant qualities.Este estudo estabelece um processo, sensível e confiável, com aplicação de cromatografia líquida de alta eficiência (HPLC) para distinguir variedades de soja convencionais de geneticamente modificadas, resistentes ao glifosato, por detecção de chiquimato nas sementes. O mecanismo de ação do glifosato é bem definido. É o único herbicida que inibe especificamente a enzima 5-enolpiruvilchiquimato-3-fosfato sintase (EPSPS, E.C. 2.5.1.19), que catalisa a condensação do chiquimato com fosfoenolpiruvato. O trabalho está baseado na concepção do chiquimato se acumular significativamente nos tecidos vegetais de soja convencional, após a inibição da EPSP sintase pelo glifosato. Em plantas não submetidas ao glifosato, o chiquimato não é facilmente detectado, pois rapidamente é metabolizado a chiquimato 3-fosfato e, a seguir, em 5-enolpiruvilchiquimato 3-fosfato, pela ação da EPSPS. Por outro lado, em plantas não geneticamente modificadas submetidas ao glifosato, a metabolização do chiquimato é prejudicada, resultando em seu acúmulo. Este metabólito pode ser detectado em quantidades extremamente baixas (na faixa de mg), por HPLC. Neste trabalho foram analisados seis contrastes diferentes, sendo cada contraste formado por uma cultivar transgênica e sua respectiva cultivar parental convencional, submetidas ou não a embebição com solução de glifosato 0,6%. As análises cromatográficas indicaram o acúmulo de chiquimato apenas em cultivares convencionais, nas quais as sementes foram previamente embebidas em solução de glifosato 0,6%. Os resultados demonstraram que a detecção de chiquimato pode ser utilizada como um método rápido e preciso na diferenciação de soja resistente ao glifosato de soja convencional.EDUFU2020-02-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4233910.14393/BJ-v36n2a2020-42339Bioscience Journal ; Vol. 36 No. 2 (2020): Mar./Apr.; 383-389Bioscience Journal ; v. 36 n. 2 (2020): Mar./Abr; 383-3891981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42339/28088Brazil; ContemporaryCopyright (c) 2020 Edicléia Aparecida Bonini , Rogério Marchiosi, Patricia da Costa Zonetti, Luiz Henrique Saes Zobiole, Osvaldo Ferrarese-Filhohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBonini , Edicléia AparecidaMarchiosi, RogérioZonetti, Patricia da CostaZobiole, Luiz Henrique SaesFerrarese-Filho, Osvaldo2022-06-22T12:29:05Zoai:ojs.www.seer.ufu.br:article/42339Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-06-22T12:29:05Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean Determinação cromatográfica de chiquimato para identificação de soja convenciolnal e resistente ao glifosato |
title |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean |
spellingShingle |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean Bonini , Edicléia Aparecida Glycine max L. Merril. Transgenesis RR soy HPLC Agricultural Sciences |
title_short |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean |
title_full |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean |
title_fullStr |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean |
title_full_unstemmed |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean |
title_sort |
Chromatographic determination of shikimate for identification of conventional soybean and glyphosate resistant soybean |
author |
Bonini , Edicléia Aparecida |
author_facet |
Bonini , Edicléia Aparecida Marchiosi, Rogério Zonetti, Patricia da Costa Zobiole, Luiz Henrique Saes Ferrarese-Filho, Osvaldo |
author_role |
author |
author2 |
Marchiosi, Rogério Zonetti, Patricia da Costa Zobiole, Luiz Henrique Saes Ferrarese-Filho, Osvaldo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Bonini , Edicléia Aparecida Marchiosi, Rogério Zonetti, Patricia da Costa Zobiole, Luiz Henrique Saes Ferrarese-Filho, Osvaldo |
dc.subject.por.fl_str_mv |
Glycine max L. Merril. Transgenesis RR soy HPLC Agricultural Sciences |
topic |
Glycine max L. Merril. Transgenesis RR soy HPLC Agricultural Sciences |
description |
A sensitive and reliable process was established using high-performance liquid chromatography (HPLC) to distinguish conventional varieties of glyphosate-resistant genetically modified crops via shikimate detection in soybean (Glycine max L. Merril) seeds. Glyphosate has a well-defined mechanism of action. It is the only herbicide that specifically inhibits 5-enolpiruvilshikimate-3-phosphate synthase (EPSPS E.C. 2.5.1.19), which catalyzes the condensation of shikimate with phosphoenolpyruvate. This study is based on the concept that shikimate significantly accumulates in soybean plant tissues after EPSPS inhibition by glyphosate. In plants not subjected to glyphosate, shikimate is not easily detected because it quickly metabolizes into shikimate 3-phosphate and subsequently into 5-enolpiruvilshikimate 3-phosphate through the action of EPSPS. Conversely, in non-genetically modified plants subjected to glyphosate, shikimate metabolism is impaired, resulting in its accumulation. This metabolite can be detected in extremely low quantities (in the microgram range), through HPLC. In this study, six different contrasts were analyzed, each being formed by a transgenic cultivation and its parental strain, subject or not subject to the treatment of soaking with a 0.6% glyphosate solution. Chromatographic analyses indicated shikimate accumulation only in conventional cultivars with seeds previously soaked in a 0.6% glyphosate solution. Thus, this shikimate detection method can be used as a rapid and accurate means to distinguish soybeans with glyphosate-resistant qualities. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42339 10.14393/BJ-v36n2a2020-42339 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42339 |
identifier_str_mv |
10.14393/BJ-v36n2a2020-42339 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42339/28088 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 36 No. 2 (2020): Mar./Apr.; 383-389 Bioscience Journal ; v. 36 n. 2 (2020): Mar./Abr; 383-389 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069080353570816 |