Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/26333 |
Resumo: | In the search for alternative ways to control Aedes aegypti with minimal environmental impact and in a manner that preserves human health, this study sought to evaluate the larvicidal effect of the invasive and antioxidant Spermacoce latifolia plant by performing a phytochemical study. Phytochemical screenings were done according to characterization reactions and thin layer chromatography. Phenolics compounds content (Folin-Ciocalteu's) and flavonoids (AlCl3) spectrophotometric was performed, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical results revealed the presence of phenolic, flavonoid, tannin, steroid, free triterpene, coumarin, and alkaloid compounds. The content of total phenols (TPs) (482.7 ± 1.8 mg mgGA g-1) and flavonoids (165.4 ± 1.5 mg QE g-1) accounted for the antioxidant activity of 150 µg.mL-1 methanolic extract. In the proposed bioassays, groups of 25 third-stage larvae were challenged at different concentrations of plant crude extract (1.0, 0.5, 0.25, and 0.1 g L-1) of weight per volume in four replicates. In multiple concentration tests, the concentrations were selected to range from 0% to 100% mortality after 24 hours of contact with the solution. Toxicity was defined as the inhibition or total inactivity of the larvae. It was concluded that the methanol extract had an LC50 of 0.62 g L-1, indicating its potential use as a larvicide against A. aegypti and linking its activity to its phenolic and flavonoid components. |
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Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) vector controlplant active ingredients and plant insecticides.Agricultural SciencesIn the search for alternative ways to control Aedes aegypti with minimal environmental impact and in a manner that preserves human health, this study sought to evaluate the larvicidal effect of the invasive and antioxidant Spermacoce latifolia plant by performing a phytochemical study. Phytochemical screenings were done according to characterization reactions and thin layer chromatography. Phenolics compounds content (Folin-Ciocalteu's) and flavonoids (AlCl3) spectrophotometric was performed, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical results revealed the presence of phenolic, flavonoid, tannin, steroid, free triterpene, coumarin, and alkaloid compounds. The content of total phenols (TPs) (482.7 ± 1.8 mg mgGA g-1) and flavonoids (165.4 ± 1.5 mg QE g-1) accounted for the antioxidant activity of 150 µg.mL-1 methanolic extract. In the proposed bioassays, groups of 25 third-stage larvae were challenged at different concentrations of plant crude extract (1.0, 0.5, 0.25, and 0.1 g L-1) of weight per volume in four replicates. In multiple concentration tests, the concentrations were selected to range from 0% to 100% mortality after 24 hours of contact with the solution. Toxicity was defined as the inhibition or total inactivity of the larvae. It was concluded that the methanol extract had an LC50 of 0.62 g L-1, indicating its potential use as a larvicide against A. aegypti and linking its activity to its phenolic and flavonoid components.In the search for alternative ways to control Aedes aegypti with minimal environmental impact and in a manner that preserves human health, this study sought to evaluate the larvicidal effect of the invasive and antioxidant Spermacoce latifolia plant by performing a phytochemical study. Phytochemical screenings were done according to characterization reactions and thin layer chromatography. Phenolics compounds content (Folin-Ciocalteu's) and flavonoids (AlCl3) spectrophotometric was performed, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical results revealed the presence of phenolic, flavonoid, tannin, steroid, free triterpene, coumarin, and alkaloid compounds. The content of total phenols (TPs) (482.7 ± 1.8 mg mgGA g-1) and flavonoids (165.4 ± 1.5 mg QE g-1) accounted for the antioxidant activity of 150 µg.mL-1 methanolic extract. In the proposed bioassays, groups of 25 third-stage larvae were challenged at different concentrations of plant crude extract (1.0, 0.5, 0.25, and 0.1 g L-1) of weight per volume in four replicates. In multiple concentration tests, the concentrations were selected to range from 0% to 100% mortality after 24 hours of contact with the solution. Toxicity was defined as the inhibition or total inactivity of the larvae. It was concluded that the methanol extract had an LC50 of 0.62 g L-1, indicating its potential use as a larvicide against A. aegypti and linking its activity to its phenolic and flavonoid components.EDUFU2015-09-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/2633310.14393/BJ-v31n5a2015-26333Bioscience Journal ; Vol. 31 No. 5 (2015): Sept./Oct.; Agricultural SciencesBioscience Journal ; v. 31 n. 5 (2015): Sept./Oct.; Agricultural Sciences1981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/26333/17142Brazil; ContemporaryCopyright (c) 2015 Ana Claudia Oliveira Firak Cosmoski, Antonia Railda Roel, Karla Rejane de Andrade Porto, Rosemary Matias Coelho, Michel Robin Honer, Priscila Rezende Mottihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosmoski, Ana Claudia Oliveira FirakRoel, Antonia RaildaPorto, Karla Rejane de AndradeCoelho, Rosemary MatiasHoner, Michel RobinMotti, Priscila Rezende2022-05-19T14:33:44Zoai:ojs.www.seer.ufu.br:article/26333Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-19T14:33:44Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
title |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
spellingShingle |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) Cosmoski, Ana Claudia Oliveira Firak vector control plant active ingredients and plant insecticides. Agricultural Sciences |
title_short |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
title_full |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
title_fullStr |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
title_full_unstemmed |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
title_sort |
Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) |
author |
Cosmoski, Ana Claudia Oliveira Firak |
author_facet |
Cosmoski, Ana Claudia Oliveira Firak Roel, Antonia Railda Porto, Karla Rejane de Andrade Coelho, Rosemary Matias Honer, Michel Robin Motti, Priscila Rezende |
author_role |
author |
author2 |
Roel, Antonia Railda Porto, Karla Rejane de Andrade Coelho, Rosemary Matias Honer, Michel Robin Motti, Priscila Rezende |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cosmoski, Ana Claudia Oliveira Firak Roel, Antonia Railda Porto, Karla Rejane de Andrade Coelho, Rosemary Matias Honer, Michel Robin Motti, Priscila Rezende |
dc.subject.por.fl_str_mv |
vector control plant active ingredients and plant insecticides. Agricultural Sciences |
topic |
vector control plant active ingredients and plant insecticides. Agricultural Sciences |
description |
In the search for alternative ways to control Aedes aegypti with minimal environmental impact and in a manner that preserves human health, this study sought to evaluate the larvicidal effect of the invasive and antioxidant Spermacoce latifolia plant by performing a phytochemical study. Phytochemical screenings were done according to characterization reactions and thin layer chromatography. Phenolics compounds content (Folin-Ciocalteu's) and flavonoids (AlCl3) spectrophotometric was performed, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical results revealed the presence of phenolic, flavonoid, tannin, steroid, free triterpene, coumarin, and alkaloid compounds. The content of total phenols (TPs) (482.7 ± 1.8 mg mgGA g-1) and flavonoids (165.4 ± 1.5 mg QE g-1) accounted for the antioxidant activity of 150 µg.mL-1 methanolic extract. In the proposed bioassays, groups of 25 third-stage larvae were challenged at different concentrations of plant crude extract (1.0, 0.5, 0.25, and 0.1 g L-1) of weight per volume in four replicates. In multiple concentration tests, the concentrations were selected to range from 0% to 100% mortality after 24 hours of contact with the solution. Toxicity was defined as the inhibition or total inactivity of the larvae. It was concluded that the methanol extract had an LC50 of 0.62 g L-1, indicating its potential use as a larvicide against A. aegypti and linking its activity to its phenolic and flavonoid components. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/26333 10.14393/BJ-v31n5a2015-26333 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/26333 |
identifier_str_mv |
10.14393/BJ-v31n5a2015-26333 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/26333/17142 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 31 No. 5 (2015): Sept./Oct.; Agricultural Sciences Bioscience Journal ; v. 31 n. 5 (2015): Sept./Oct.; Agricultural Sciences 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069075296288768 |