Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields

Detalhes bibliográficos
Autor(a) principal: Zancan, Willian Luis Antonio
Data de Publicação: 2015
Outros Autores: Steadman, James R., Higgins, Rebecca, Jhala, Rachana, Machado, José da Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/26247
Resumo: Sclerotinia sclerotiorum, infection of bean fields, has increased in Brazil. Fungicides application is the control strategy used due to lack of cultivars with complete disease resistance. To guide the use of isolates in resistance screening 25 S. sclerotiorum isolates from Brazilian dry bean fields were characterized using microsatellite markers, mycelial compatibility groups (MCGs) and aggressiveness. Microsatellite primer pairs were used to identify polymorphisms among the S. sclerotiorum isolates and MCGs were determined from interaction of all isolates grown side-by-side. Aggressiveness was derived from a straw test where fungal mycelium was placed over a cut bean stem and rated for disease progress. Data from microsatellite profiles grouped the 25 isolates into four clusters and seven MCGs were identified. No association among host cultivar and cluster or MCG of isolates was observed. For MCGs, 57% contained isolates sampled frequently over multiple locations and 43% contained isolates unique to locations. There were significant differences among isolates in aggressiveness within and between MCGs. The most aggressive isolates in resistance screening will be helpful in the identification of higher levels of resistance in bean germplasm/lines.
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spelling Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields Aggressivenessmicrosatellitesresistancewhite moldAgricultural SciencesSclerotinia sclerotiorum, infection of bean fields, has increased in Brazil. Fungicides application is the control strategy used due to lack of cultivars with complete disease resistance. To guide the use of isolates in resistance screening 25 S. sclerotiorum isolates from Brazilian dry bean fields were characterized using microsatellite markers, mycelial compatibility groups (MCGs) and aggressiveness. Microsatellite primer pairs were used to identify polymorphisms among the S. sclerotiorum isolates and MCGs were determined from interaction of all isolates grown side-by-side. Aggressiveness was derived from a straw test where fungal mycelium was placed over a cut bean stem and rated for disease progress. Data from microsatellite profiles grouped the 25 isolates into four clusters and seven MCGs were identified. No association among host cultivar and cluster or MCG of isolates was observed. For MCGs, 57% contained isolates sampled frequently over multiple locations and 43% contained isolates unique to locations. There were significant differences among isolates in aggressiveness within and between MCGs. The most aggressive isolates in resistance screening will be helpful in the identification of higher levels of resistance in bean germplasm/lines.EDUFU2015-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/2624710.14393/BJ-v31n4a2015-26247Bioscience Journal ; Vol. 31 No. 4 (2015): July/Aug.; 1143-1151Bioscience Journal ; v. 31 n. 4 (2015): July/Aug.; 1143-11511981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/26247/16735Brazil; ContemporaryCopyright (c) 2015 Willian Luis Antonio Zancan, James R. Steadman, Rebecca Higgins, Rachana Jhala, José da Cruz Machadohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessZancan, Willian Luis AntonioSteadman, James R.Higgins, RebeccaJhala, RachanaMachado, José da Cruz2022-05-20T02:45:27Zoai:ojs.www.seer.ufu.br:article/26247Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-20T02:45:27Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
title Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
spellingShingle Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
Zancan, Willian Luis Antonio
Aggressiveness
microsatellites
resistance
white mold
Agricultural Sciences
title_short Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
title_full Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
title_fullStr Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
title_full_unstemmed Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
title_sort Genetic and aggressiveness variation among Sclerotinia sclerotiorum dry bean isolates from Brazil fields
author Zancan, Willian Luis Antonio
author_facet Zancan, Willian Luis Antonio
Steadman, James R.
Higgins, Rebecca
Jhala, Rachana
Machado, José da Cruz
author_role author
author2 Steadman, James R.
Higgins, Rebecca
Jhala, Rachana
Machado, José da Cruz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zancan, Willian Luis Antonio
Steadman, James R.
Higgins, Rebecca
Jhala, Rachana
Machado, José da Cruz
dc.subject.por.fl_str_mv Aggressiveness
microsatellites
resistance
white mold
Agricultural Sciences
topic Aggressiveness
microsatellites
resistance
white mold
Agricultural Sciences
description Sclerotinia sclerotiorum, infection of bean fields, has increased in Brazil. Fungicides application is the control strategy used due to lack of cultivars with complete disease resistance. To guide the use of isolates in resistance screening 25 S. sclerotiorum isolates from Brazilian dry bean fields were characterized using microsatellite markers, mycelial compatibility groups (MCGs) and aggressiveness. Microsatellite primer pairs were used to identify polymorphisms among the S. sclerotiorum isolates and MCGs were determined from interaction of all isolates grown side-by-side. Aggressiveness was derived from a straw test where fungal mycelium was placed over a cut bean stem and rated for disease progress. Data from microsatellite profiles grouped the 25 isolates into four clusters and seven MCGs were identified. No association among host cultivar and cluster or MCG of isolates was observed. For MCGs, 57% contained isolates sampled frequently over multiple locations and 43% contained isolates unique to locations. There were significant differences among isolates in aggressiveness within and between MCGs. The most aggressive isolates in resistance screening will be helpful in the identification of higher levels of resistance in bean germplasm/lines.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/26247
10.14393/BJ-v31n4a2015-26247
url https://seer.ufu.br/index.php/biosciencejournal/article/view/26247
identifier_str_mv 10.14393/BJ-v31n4a2015-26247
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/26247/16735
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 31 No. 4 (2015): July/Aug.; 1143-1151
Bioscience Journal ; v. 31 n. 4 (2015): July/Aug.; 1143-1151
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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