Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/36825 |
Resumo: | The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development. |
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Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta Efeito do tempo de gestação em expressão de ARNm de transportadores de ácidos graxos mrna em placenta bovinos Fatty acid binding proteinFetal programingFatty acidsBiological SciencesThe objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.O objetivo do estudo foi avaliar o efeito do tempo de gestação na expressão de mRNA de transportador de ácidos graxos em placenta bovina materna e fetal. Foram colhidas amostras de placentas de 12 vacas em diferentes terços da gestação (n = 4 por terço) no abatedouro para medir a expressão de mRNA de FATP-1, FATP-4, FABP-1 no lado materno (carúnculos) e fetal (cotilédones). Uma vez que a placenta foi removida, 1 cm2 foi dissecado e, dividido em carúnculos e cotilédones, armazenado em tubos estéreis, caiu em nitrogenio líquido e mantido a -80 ° C até a análise rtPCR. Os dados foram analisados como um delineamento inteiramente casualizado com esquema fatorial 3 x 2, utilizando o procedimento misto (SAS 9.3) com medidas repetidas no espaço. O tempo de gestação, o lado da placenta e sua interação foram fatores fixos, enquanto que o animal foi um fator aleatório. Houve um tempo de interação do tratamento (P <0,01) na expressão de mRNA de FATP-1 devido a uma maior expressão de mRNA em cotilédones no primeiro terço da gestação em comparação com a concentração em carúnculas. No segundo e terceiro terços da gestação, aexpressão de mRNA nos cotilédones diminuiu, atingindo uma expressão semelhante à observada em carúnculas. A proteína de transporte de ácidos graxos 4 e a expressão de mRNA de FABP-1 não foram diferentes (P> 0,1). Concluímos que a FATP-1 poderia desempenhar um papel importante no transporte de ácidos graxos durante o desenvolvimento fetal precoce.EDUFU2017-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3682510.14393/BJ-v34n1a2018-36825Bioscience Journal ; Vol. 34 No. 1 (2018): Jan./Feb.; 180-185Bioscience Journal ; v. 34 n. 1 (2018): Jan./Feb.; 180-1851981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/36825/21467Brazil; ContemporaryCopyright (c) 2017 Ramiro Desantadina, Silvina Quintana, Mariana Inés Recavarren, Alejandro Enrique Rellinghttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDesantadina, RamiroQuintana, SilvinaRecavarren, Mariana InésRelling, Alejandro Enrique2022-02-11T02:44:00Zoai:ojs.www.seer.ufu.br:article/36825Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-11T02:44Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta Efeito do tempo de gestação em expressão de ARNm de transportadores de ácidos graxos mrna em placenta bovinos |
title |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta |
spellingShingle |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta Desantadina, Ramiro Fatty acid binding protein Fetal programing Fatty acids Biological Sciences |
title_short |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta |
title_full |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta |
title_fullStr |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta |
title_full_unstemmed |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta |
title_sort |
Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta |
author |
Desantadina, Ramiro |
author_facet |
Desantadina, Ramiro Quintana, Silvina Recavarren, Mariana Inés Relling, Alejandro Enrique |
author_role |
author |
author2 |
Quintana, Silvina Recavarren, Mariana Inés Relling, Alejandro Enrique |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Desantadina, Ramiro Quintana, Silvina Recavarren, Mariana Inés Relling, Alejandro Enrique |
dc.subject.por.fl_str_mv |
Fatty acid binding protein Fetal programing Fatty acids Biological Sciences |
topic |
Fatty acid binding protein Fetal programing Fatty acids Biological Sciences |
description |
The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/36825 10.14393/BJ-v34n1a2018-36825 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/36825 |
identifier_str_mv |
10.14393/BJ-v34n1a2018-36825 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/36825/21467 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 34 No. 1 (2018): Jan./Feb.; 180-185 Bioscience Journal ; v. 34 n. 1 (2018): Jan./Feb.; 180-185 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069077675507712 |