Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells

Detalhes bibliográficos
Autor(a) principal: Oliveira, Douglas Souza
Data de Publicação: 2017
Tipo de documento: Trabalho de conclusão de curso
Idioma: eng
Título da fonte: Repositório Institucional da UFU
Texto Completo: https://repositorio.ufu.br/handle/123456789/22673
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cellsSabicea brasiliensisAntioxidant activityEthy acetate fractionATP hydrolysisVascular smooth muscle cellsCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALCNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoTrabalho de Conclusão de Curso (Graduação)This study evaluated antioxidant activity of Sabicea brasiliensis root extract and fractions and it is effect on adenine nucleotide hydrolyses in vascular smooth muscle cells. Percentage of antioxidant activities of crude extract, ethyl acetate and hydro-methanolic fractions were determinate by inhibition of the DPPH and ABTS radical in comparison with BHT standard. Results for DPPH assay showed highest antioxidant activity performance for crude extract (76%), followed by ethyl acetate (46%) and hydro-methanolic (23%) fractions. These results were corroborated by ABTS assay. Total phenolic content was evaluated by Folin-Ciocalteau colorimetric method and showed a significant amount of those molecules in crude extract (140.6 ± 7.7 mg GAE.g-1), followed by ethyl acetate (67.1 ± 4.7 mg GAE.g-1) and hydro-methanolic fraction (13.5±8.2 GAE.g-1). Vascular smooth muscular cells were treated with ethyl acetate fraction at 62.5; 125; 250 and 500 μg·mL−1 and demonstrated an inhibition on ATP hydrolysis (56,65%), evidencing that the Sabicea brasiliensis can modulate purine levels in vascular tissue.Universidade Federal de UberlândiaBrasilBiotecnologiaFürstenau, Cristina Ribashttp://lattes.cnpq.br/6394110096506077Oliveira, Douglas Souza2018-10-19T18:34:00Z2018-10-19T18:34:00Z2017-07-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfOLIVEIRA, Douglas Souza. Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells. 2017. 26 f. Trabaho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2017.https://repositorio.ufu.br/handle/123456789/22673enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2021-12-02T14:19:01Zoai:repositorio.ufu.br:123456789/22673Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2021-12-02T14:19:01Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
title Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
spellingShingle Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
Oliveira, Douglas Souza
Sabicea brasiliensis
Antioxidant activity
Ethy acetate fraction
ATP hydrolysis
Vascular smooth muscle cells
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
title_full Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
title_fullStr Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
title_full_unstemmed Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
title_sort Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells
author Oliveira, Douglas Souza
author_facet Oliveira, Douglas Souza
author_role author
dc.contributor.none.fl_str_mv Fürstenau, Cristina Ribas
http://lattes.cnpq.br/6394110096506077
dc.contributor.author.fl_str_mv Oliveira, Douglas Souza
dc.subject.por.fl_str_mv Sabicea brasiliensis
Antioxidant activity
Ethy acetate fraction
ATP hydrolysis
Vascular smooth muscle cells
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
topic Sabicea brasiliensis
Antioxidant activity
Ethy acetate fraction
ATP hydrolysis
Vascular smooth muscle cells
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2017
dc.date.none.fl_str_mv 2017-07-20
2018-10-19T18:34:00Z
2018-10-19T18:34:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLIVEIRA, Douglas Souza. Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells. 2017. 26 f. Trabaho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2017.
https://repositorio.ufu.br/handle/123456789/22673
identifier_str_mv OLIVEIRA, Douglas Souza. Antioxidant activity of sabicea brasiliensis and its effect on adenine nucleotides metabolism in vascular smooth muscle cells. 2017. 26 f. Trabaho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2017.
url https://repositorio.ufu.br/handle/123456789/22673
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Biotecnologia
publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Biotecnologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFU
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Repositório Institucional da UFU
collection Repositório Institucional da UFU
repository.name.fl_str_mv Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv diinf@dirbi.ufu.br
_version_ 1805569575278870528

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