Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/15750 https://doi.org/10.14393/ufu.te.2014.84 |
Resumo: | The main role of bees in nature is pollination of angiosperms, because of it bees are responsible for biological diversity maintenance and food production as well. Brazilian stingless bees (Meliponini) pollinate 40-90% of native trees and are found worldwide, wherein Brazil is the main representative of these bees. The Melipona scutellaris species (popularly known as the uruçu do nordeste ) is distributed geographically from Bahia to Rio Grande do Norte State, in the Zona da Mata region. The understanding of caste differentiation in Melipona scutellaris is extremely important for conservation and management of this bee. In 1950, Kerr proposed the model two genes/two-locus to explain the caste differentiation in Melipona; when well fed double heterozygous larvae develop into queens and underfed larvae or homozygous for these genes become workers, as a result of low production of Juvenile Hormone. Antennae is the main organ of bee olfactory system playing an important role in mantaining of colony social organization and in the performance of caste and sexes as well. The antennae houses a great diversity of sensilla, which are very important to bee chemical communication as receptors of compounds in the air such as pheromones, leading to typical behavioral responses of individuals, species, genera or families. The second chapter we show the results from scanning electron microscopy which demonstrates a heterogeneous distribution pattern of sensilla along the antenna. The main types of sensillum found in the antennae of M. scutellaris were: trichodea, basiconica, placodea, coeloconica, ampullacea and campaniformia. The most abundant sensilla found were trichodea and placoid in both castes and sexes. The ampullacea and coeloconica sensilla were found only in the distal flagellomeres of females and campiniformes and basiconica sensilla were not found in the analyzed antennae male. Larvae pre-defecant showed structures that are morphologically similar to sensilla. These structures has pores at its base and were found in many parts of the head area, which may represent an important gateway of volatile compounds from the larval food. xvii These compounds, possibly can activate genes involved in caste differentiation of Melipona scutellaris. The third chapter introduces the detection, cloning and sequencing of transcripts of the MscuCSP2, MscuCSP6, MscuOBP4 and MscuOBP8 genes from Melipona scutellaris larvae. We detected the MscuOBP8 protein in the L2, L3 (1-3), LPD LD larval stages of development using a monoclonal antibody selected by phage display. The 3D structure of the MscuOBP8 protein showed an internal cavity composed of hydrophobic amino acids confirming its binding function to hydrophobic compounds such as volatile substances. According to our data, the morphology of the head and especially adult antenna of the pre-defecating larvae (stage responsive to the action of juvenile hormone) and also the caracterization of genes and proteins of olfactory system in larvae of Melipona scutellaris can be correlated with caste differentiation mechanism in Melipona scutellaris proposed by Kerr. |
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2016-06-22T18:43:30Z2015-02-112016-06-22T18:43:30Z2014-07-31CARVALHO, Washington João de. Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini). 2014. 118 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2014. DOI https://doi.org/10.14393/ufu.te.2014.84https://repositorio.ufu.br/handle/123456789/15750https://doi.org/10.14393/ufu.te.2014.84The main role of bees in nature is pollination of angiosperms, because of it bees are responsible for biological diversity maintenance and food production as well. Brazilian stingless bees (Meliponini) pollinate 40-90% of native trees and are found worldwide, wherein Brazil is the main representative of these bees. The Melipona scutellaris species (popularly known as the uruçu do nordeste ) is distributed geographically from Bahia to Rio Grande do Norte State, in the Zona da Mata region. The understanding of caste differentiation in Melipona scutellaris is extremely important for conservation and management of this bee. In 1950, Kerr proposed the model two genes/two-locus to explain the caste differentiation in Melipona; when well fed double heterozygous larvae develop into queens and underfed larvae or homozygous for these genes become workers, as a result of low production of Juvenile Hormone. Antennae is the main organ of bee olfactory system playing an important role in mantaining of colony social organization and in the performance of caste and sexes as well. The antennae houses a great diversity of sensilla, which are very important to bee chemical communication as receptors of compounds in the air such as pheromones, leading to typical behavioral responses of individuals, species, genera or families. The second chapter we show the results from scanning electron microscopy which demonstrates a heterogeneous distribution pattern of sensilla along the antenna. The main types of sensillum found in the antennae of M. scutellaris were: trichodea, basiconica, placodea, coeloconica, ampullacea and campaniformia. The most abundant sensilla found were trichodea and placoid in both castes and sexes. The ampullacea and coeloconica sensilla were found only in the distal flagellomeres of females and campiniformes and basiconica sensilla were not found in the analyzed antennae male. Larvae pre-defecant showed structures that are morphologically similar to sensilla. These structures has pores at its base and were found in many parts of the head area, which may represent an important gateway of volatile compounds from the larval food. xvii These compounds, possibly can activate genes involved in caste differentiation of Melipona scutellaris. The third chapter introduces the detection, cloning and sequencing of transcripts of the MscuCSP2, MscuCSP6, MscuOBP4 and MscuOBP8 genes from Melipona scutellaris larvae. We detected the MscuOBP8 protein in the L2, L3 (1-3), LPD LD larval stages of development using a monoclonal antibody selected by phage display. The 3D structure of the MscuOBP8 protein showed an internal cavity composed of hydrophobic amino acids confirming its binding function to hydrophobic compounds such as volatile substances. According to our data, the morphology of the head and especially adult antenna of the pre-defecating larvae (stage responsive to the action of juvenile hormone) and also the caracterization of genes and proteins of olfactory system in larvae of Melipona scutellaris can be correlated with caste differentiation mechanism in Melipona scutellaris proposed by Kerr.O principal papel das abelhas na natureza é a polinização de angiospermas, com papel fundamental na manutenção da diversidade biológica, bem como na produção de alimentos de modo geral. As abelhas brasileiras sem ferrão (Meliponini), polinizam de 40 a 90% das árvores nativas e estão presentes em todo o mundo, sendo o Brasil o principal representante dessas abelhas. A espécie Melipona scutellaris (popularmente conhecida como uruçu do Nordeste) está distribuída na região da Zona da Mata, ocorrendo desde a Bahia até o Rio Grande do Norte. O entendimento sobre a diferenciação de castas em Melipona scutellaris é um aspecto extremamente importante para a conservação e manejo dessa abelha, principalmente no Brasil. Em 1950, Kerr propôs um modelo, para explicar a diferenciação de casta em Melipona, de dois genes/dois lócus, de modo que larvas duplo heterozigotas, quando bem alimentadas, desenvolvem-se em rainhas e larvas mal alimentadas ou homozigotas para esses genes, tornam-se operárias, em consequência de baixa produção de Hormônio Juvenil. Para a organização social da colônia e desempenho adequado dos papéis das castas e sexos, as abelhas apresentam um elaborado sistema olfatório, do qual a antena é o principal órgão sensorial, abrigando grande diversidade de sensilas. As sensilas têm papel na comunicação química intra e interespecífica, atuando como receptores de compostos químicos no ar, como os feromônios, levando à respostas comportamentais característico de indivíduos, espécies, famílias ou gêneros. No segundo capítulo apresentamos, de acordo com as imagens de microscopia de eletrônica de varredura, um padrão heterogêneo de distribuição das sensilas ao longo da antena. Os principais tipos de sensilas identificados nas antenas de M. scutellaris foram: tricóide, basicônica, placóide, celocônica, ampulácea e campiniforme, sendo que as sensilas tricóides e placóides foram as mais abundantes em todas as castas e sexo. As sensilas ampulácea e celocônica foram encontradas apenas nos flagelômeros distais das fêmeas e xv campiniformes e basicônicas não foram encontradas nas antenas de macho analisadas. As larvas LPD apresentaram estruturas morfologicamente semelhantes à sensilas, com poros em sua base, em diversas regiões da área analisada, que podem representar uma importante porta de entrada de compostos voláteis presentes no alimento larval que possam ativar genes envolvidos na diferenciação de castas de Melipona scutellaris. No terceiro capítulo, nós detectamos transcritos, clonamos e sequenciamentos os genes MscuCSP2, MscuCSP6, MscuOBP4 e MscuOBP8 em larvas de M. scutellaris. Selecionamos um anticorpo monoclonal por phage display que nos permitiu detectar a proteína MscuOBP8 nos estágios L2, L3 (1- 3), LPD e LD do desenvolvimento larval. A estrutura 3D da proteína MscuOBP8 apresentou cavidade interna composta por aminoácidos hidrofóbicos corroborando com sua função carreadora de compostos hidrofóbicos tais como, substâncias voláteis. Neste contexto, este trabalho apresenta a morfologia da antena de adultos e, principalmente, da porção anterior de larva do estágio pré-defecante do desenvolvimento (sensível à ação do hormônio juvenil) e também a caracterização de genes e proteínas do sistema olfatório em larva de Melipona scutellaris a fim de correlacioná-los com o mecanismo de diferenciação de castas em Melipona scutellaris proposto por Kerr.Fundação de Amparo a Pesquisa do Estado de Minas GeraisDoutor em Genética e Bioquímicaapplication/pdfporUniversidade Federal de UberlândiaPrograma de Pós-graduação em Genética e BioquímicaUFUBRCiências BiológicasMelipona scutellarisCNPQ::CIENCIAS BIOLOGICAS::GENETICAIdentificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisLeal, WalterUeira-Vieira, Carloshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706664A7Bonetti, Ana Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783660P9Augusto, Solange Cristinahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728013Y8Carvalho-zilse, Gislene Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728328H6Silva, Carlos Gustavo Nunes dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768602U4http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4562314D0Carvalho, Washington João de81763411d6753981-8e41-42fb-9dfa-f52cb5852ff1info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFUTHUMBNAILIdentificacaoCaracterizacaoGenes_parte 1.pdf.jpgIdentificacaoCaracterizacaoGenes_parte 1.pdf.jpgGenerated 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dc.title.por.fl_str_mv |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
title |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
spellingShingle |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) Carvalho, Washington João de Melipona scutellaris CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
title_full |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
title_fullStr |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
title_full_unstemmed |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
title_sort |
Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini) |
author |
Carvalho, Washington João de |
author_facet |
Carvalho, Washington João de |
author_role |
author |
dc.contributor.advisor-co1.fl_str_mv |
Leal, Walter |
dc.contributor.advisor1.fl_str_mv |
Ueira-Vieira, Carlos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706664A7 |
dc.contributor.referee1.fl_str_mv |
Bonetti, Ana Maria |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783660P9 |
dc.contributor.referee2.fl_str_mv |
Augusto, Solange Cristina |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728013Y8 |
dc.contributor.referee3.fl_str_mv |
Carvalho-zilse, Gislene Almeida |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728328H6 |
dc.contributor.referee4.fl_str_mv |
Silva, Carlos Gustavo Nunes da |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768602U4 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4562314D0 |
dc.contributor.author.fl_str_mv |
Carvalho, Washington João de |
contributor_str_mv |
Leal, Walter Ueira-Vieira, Carlos Bonetti, Ana Maria Augusto, Solange Cristina Carvalho-zilse, Gislene Almeida Silva, Carlos Gustavo Nunes da |
dc.subject.por.fl_str_mv |
Melipona scutellaris |
topic |
Melipona scutellaris CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
description |
The main role of bees in nature is pollination of angiosperms, because of it bees are responsible for biological diversity maintenance and food production as well. Brazilian stingless bees (Meliponini) pollinate 40-90% of native trees and are found worldwide, wherein Brazil is the main representative of these bees. The Melipona scutellaris species (popularly known as the uruçu do nordeste ) is distributed geographically from Bahia to Rio Grande do Norte State, in the Zona da Mata region. The understanding of caste differentiation in Melipona scutellaris is extremely important for conservation and management of this bee. In 1950, Kerr proposed the model two genes/two-locus to explain the caste differentiation in Melipona; when well fed double heterozygous larvae develop into queens and underfed larvae or homozygous for these genes become workers, as a result of low production of Juvenile Hormone. Antennae is the main organ of bee olfactory system playing an important role in mantaining of colony social organization and in the performance of caste and sexes as well. The antennae houses a great diversity of sensilla, which are very important to bee chemical communication as receptors of compounds in the air such as pheromones, leading to typical behavioral responses of individuals, species, genera or families. The second chapter we show the results from scanning electron microscopy which demonstrates a heterogeneous distribution pattern of sensilla along the antenna. The main types of sensillum found in the antennae of M. scutellaris were: trichodea, basiconica, placodea, coeloconica, ampullacea and campaniformia. The most abundant sensilla found were trichodea and placoid in both castes and sexes. The ampullacea and coeloconica sensilla were found only in the distal flagellomeres of females and campiniformes and basiconica sensilla were not found in the analyzed antennae male. Larvae pre-defecant showed structures that are morphologically similar to sensilla. These structures has pores at its base and were found in many parts of the head area, which may represent an important gateway of volatile compounds from the larval food. xvii These compounds, possibly can activate genes involved in caste differentiation of Melipona scutellaris. The third chapter introduces the detection, cloning and sequencing of transcripts of the MscuCSP2, MscuCSP6, MscuOBP4 and MscuOBP8 genes from Melipona scutellaris larvae. We detected the MscuOBP8 protein in the L2, L3 (1-3), LPD LD larval stages of development using a monoclonal antibody selected by phage display. The 3D structure of the MscuOBP8 protein showed an internal cavity composed of hydrophobic amino acids confirming its binding function to hydrophobic compounds such as volatile substances. According to our data, the morphology of the head and especially adult antenna of the pre-defecating larvae (stage responsive to the action of juvenile hormone) and also the caracterization of genes and proteins of olfactory system in larvae of Melipona scutellaris can be correlated with caste differentiation mechanism in Melipona scutellaris proposed by Kerr. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-31 |
dc.date.available.fl_str_mv |
2015-02-11 2016-06-22T18:43:30Z |
dc.date.accessioned.fl_str_mv |
2016-06-22T18:43:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARVALHO, Washington João de. Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini). 2014. 118 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2014. DOI https://doi.org/10.14393/ufu.te.2014.84 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufu.br/handle/123456789/15750 |
dc.identifier.doi.por.fl_str_mv |
https://doi.org/10.14393/ufu.te.2014.84 |
identifier_str_mv |
CARVALHO, Washington João de. Identificação e caracterização de genes e seus produtos protéicos do sistema olfatório e morfologia de antena da abelha sem ferrão Melipona scutellaris (Apidae: Meliponini). 2014. 118 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2014. DOI https://doi.org/10.14393/ufu.te.2014.84 |
url |
https://repositorio.ufu.br/handle/123456789/15750 https://doi.org/10.14393/ufu.te.2014.84 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Genética e Bioquímica |
dc.publisher.initials.fl_str_mv |
UFU |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ciências Biológicas |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFU instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
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Universidade Federal de Uberlândia (UFU) |
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UFU |
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UFU |
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Repositório Institucional da UFU |
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Repositório Institucional da UFU |
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repository.name.fl_str_mv |
Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU) |
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diinf@dirbi.ufu.br |
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1802110323657801728 |