Transferibilidade de primers microssatélites entre espécies vegetais

• Autor(a) principal: Soares, Thais Santos
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/27824
• topic: Marcadores moleculares; SSR; Plantas; CNPQ::CIENCIAS AGRARIAS

Microsatellites or Simple Repeat Sequences (SSR) are effective genetic markers for detailed genomic analysis. They stand out for their codominant inheritance, abundance in the genome, good reproducibility, high polymorphism and ease of detection by polymerase chain reaction (PCR). However, the process of obtaining microsatellite primers requires skilled labor and has a relatively high cost, limiting their use for species with less commercial expression. An alternative is to perform SSR primer transferability between similar species. In this context, the work was carried out in order to analyze, from the literature, the occurrence of microsatellite primers transferability between plant species. The bibliographical study of publications in journals, dissertations and theses was performed. Source identification was performed using bibliographic databases from Scielo, NCBI, Embrapa and Repositories, selecting only those that met the inclusion criteria defined in this study. Only publications that answered the study question, published from 2007 to 2019 and in Portuguese and English languages were included. The sample was therefore made up of 18 publications. The present study concludes that transferability occurs successfully, especially when the species are of the same genus, offering the possibility and use of primers in genetic analysis of species still under study. Therefore, the use of transferability represents a very important resource in scientific research, contributing to the development of scientific knowledge, especially for species of lower economic interest.