Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect

Detalhes bibliográficos
Autor(a) principal: Mota, Danielly Christine Adriani Maia
Data de Publicação: 2017
Tipo de documento: Trabalho de conclusão de curso
Idioma: eng
Título da fonte: Repositório Institucional da UFU
Texto Completo: https://repositorio.ufu.br/handle/123456789/20358
Resumo: CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
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spelling Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose EffectMorinda citrifoliaCysteine proteaseproteolytic activityToxoplasma gondiiCNPQ::CIENCIAS DA SAUDECAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoTrabalho de Conclusão de Curso (Graduação)Morinda citrifolia, known as Noni, is used in folk medicine with several therapeutic applications. The present study investigated the enzymatic and biological characteristics of the papain-like cysteine proteases (PLCP) from M. citrifolia seed. Its enzyme was purified in two-step by DEAE-Sephacel and Phenyl-Sepharose CL-4B (10 × 0.6 cm) columns. PLCP enzyme demonstrated an apparent molecular mass of 51 kDa on SDS-PAGE 14%. The proteolytic activity in the presence of fibrinogen with the PLPC of M. citrifolia demonstrated total hydrolysis of the fibrinogen chains, occurring in the concentration of 20 μg; with the optimum pH of 7.0; temperatures range from room temperature to 50°C; better activity with calcium ions and the effect of inhibition appeared in the presence of Leupeptin. There was no platelet aggregation or coagulation activity. PLCP enzyme was not cytotoxic for cells such as Human Foreskin Fibroblasts (HFF) and murine embryo fibroblasts strain NIH/Swiss (NIH/3T3) macrophages and showed to be effective when tested directly against Toxoplasma gondii infection in vitro. In that sense, this study indicates perspectives for pharmacological applications of M. citrifolia enzymes.Universidade Federal de UberlândiaBrasilBiomedicinaSantiago, Fernanda Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769119A0Naves, Karinne Spirandelli Carvalhohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762339J3Junior, Jair Pereira da Cunhahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795802Y5Mota, Danielly Christine Adriani Maia2018-01-24T18:14:06Z2018-01-24T18:14:06Z2017-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfMOTA, Danielly Christine Adriani Maia. Isolation and characterization of papain-like cysteine proteinases from Morinda citrifolia seed with anti-toxoplasmose effect. 2017. 23 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Faculdade de Ciências Biomédicas, Universidade Federal de Uberlândia. Uberlândia, 2017.https://repositorio.ufu.br/handle/123456789/20358enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2018-01-24T18:14:07Zoai:repositorio.ufu.br:123456789/20358Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2018-01-24T18:14:07Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
title Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
spellingShingle Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
Mota, Danielly Christine Adriani Maia
Morinda citrifolia
Cysteine protease
proteolytic activity
Toxoplasma gondii
CNPQ::CIENCIAS DA SAUDE
title_short Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
title_full Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
title_fullStr Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
title_full_unstemmed Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
title_sort Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect
author Mota, Danielly Christine Adriani Maia
author_facet Mota, Danielly Christine Adriani Maia
author_role author
dc.contributor.none.fl_str_mv Santiago, Fernanda Maria
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769119A0
Naves, Karinne Spirandelli Carvalho
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762339J3
Junior, Jair Pereira da Cunha
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795802Y5
dc.contributor.author.fl_str_mv Mota, Danielly Christine Adriani Maia
dc.subject.por.fl_str_mv Morinda citrifolia
Cysteine protease
proteolytic activity
Toxoplasma gondii
CNPQ::CIENCIAS DA SAUDE
topic Morinda citrifolia
Cysteine protease
proteolytic activity
Toxoplasma gondii
CNPQ::CIENCIAS DA SAUDE
description CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
publishDate 2017
dc.date.none.fl_str_mv 2017-12-20
2018-01-24T18:14:06Z
2018-01-24T18:14:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MOTA, Danielly Christine Adriani Maia. Isolation and characterization of papain-like cysteine proteinases from Morinda citrifolia seed with anti-toxoplasmose effect. 2017. 23 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Faculdade de Ciências Biomédicas, Universidade Federal de Uberlândia. Uberlândia, 2017.
https://repositorio.ufu.br/handle/123456789/20358
identifier_str_mv MOTA, Danielly Christine Adriani Maia. Isolation and characterization of papain-like cysteine proteinases from Morinda citrifolia seed with anti-toxoplasmose effect. 2017. 23 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Faculdade de Ciências Biomédicas, Universidade Federal de Uberlândia. Uberlândia, 2017.
url https://repositorio.ufu.br/handle/123456789/20358
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Biomedicina
publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Biomedicina
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFU
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Repositório Institucional da UFU
collection Repositório Institucional da UFU
repository.name.fl_str_mv Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv diinf@dirbi.ufu.br
_version_ 1805569647214329856

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