Desenvolvimento de biossensor enzimático para diagnóstico de lesão no tecido hepático

• Autor(a) principal: Paraiso, Lara Ferreira
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/15834
• topic: Eletroquímica; Fígado doenças; Biossensores; Alanine aminotransferase; Modified electrode; Pyruvate oxydase; Poly(4-aminophenol); CNPQ::CIENCIAS BIOLOGICAS::GENETICA

High concentrations of alanine aminotransferase (ALT) in human serum indicate hepatic disorders. This paper reports a new procedure for ALT determination using pyruvate oxidase (PyO) immobilized onto graphite electrode modified with poly(4-aminophenol) and 4-aminoantypirine as an indicator of enzymatic system. The characterization of the resulting bioelectrode was performed using atomic force microscopy (AFM) techniques. AFM images indicate that the enzyme was successfully incorporated onto the modified graphite electrode. The bioelectrode obtained showed attractive characteristics such as a short response time (about 200 s), a low detection limit to ALT (0.003U/L) and good stability (95% of response after 4 weeks). The combination of enzymatic assay for ALT and modified electrode with poly(4-aminophenol) is a promising approach towards the development of a diagnosis kit for hepatic disease using electrochemical detection.