Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/17006 https://doi.org/10.14393/ufu.di.2015.46 |
Resumo: | Non carious cervical lesions (NCCLs) are routine findings in clinical practice and are described as the loss of tooth tissue close to the cementum enamel junction. NCCLs have multifactorial etiology with main factors involved are stress, biocorrosion and friction. The different associations of these factors can lead to the weakening of the tooth structure in the cervical region, generating NCCLs. The aim of this study was to evaluate the association of etiologic factors (stress, biocorrosion and friction) on the microstructural formation of NCCLs, using finite element analysis, laser confocal microscopy and computed microtomography (micro-CT). Two three dimensional (3D), linear and elastic models of mandibular premolars were generated for stress distribution evaluation, using the finite element analysis (FEA). Two oblique loadings (50 N), were applied on the buccal cusp slope of the model. The occlusal loading (CO) was applied on the grinding region of the buccal cusp and the buccal loading (CV) on the smooth region of the same cusp. Both loads were applied at 45̊ to the tooth long axis, simulating mandibular excursive movements. The displacement restriction was applied on the base and lateral surface of the model and the results were analyzed by the maximum principal criteria. Then, for analysis by confocal microscopy, forty intact human mandibular premolars were selected and allocated randomly into four groups, according to the factor association type (n=10): (TB) stress-biocorrosion, (TF) stress-friction, (BF) biocorrosion-friction, and (TBF) stress-biocorrosion-friction. In each group, the challenges were performed in an alternate way. The stress was simulated by cyclic fatigue loading, with specimens positioned in mechanical fatigue machine and subjected to oblique load (50N), on the smooth region of the buccal cusp for 6 x 105 cycles. The biocorrosive challenge was performed using the application of hydrochloric acid (2mL/10s). The friction (abrasion) was reproduced by using electric/powered toothbrushes (300g/20s), with Colgate Total 12 toothpaste. The roughness surface, angle and height of steps were measured before and after the treatments, using a 3D laser confocal microscope. The data were analyzed using the paired t-test, two-way analysis of variance and Tukey s test (α=0.05). Then, three specimens of each group were taken to a micro-CT for the qualitative analyses. The CV loading promoted higher concentration of tensile stress in the cervical region compared to CO. The presence of biocorrosion was an important factor to roughness changes and abrasion promoted similar behavior among the groups. The TBF association promoted the greatest alterations in tooth structures. Enamel and dentin showed distinct performance according to the different interactions. The FEA results presented relationship with the experimental findings for TF,TB, TBF groups. The TBF was the more influent association on the microstructural formation of NCCLs, for enamel. For dentin, the association of at least two factors was sufficient to generate significant tissue degradation. All studied associations of etiological factors showed potential to promote NCCLs formation. |
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2016-06-22T18:47:37Z2015-02-232016-06-22T18:47:37Z2015-02-12ZEOLA, Livia Fávaro. Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas. 2015. 85 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Uberlândia, Uberlândia, 2015. Disponível em: https://doi.org/10.14393/ufu.di.2015.46https://repositorio.ufu.br/handle/123456789/17006https://doi.org/10.14393/ufu.di.2015.46Non carious cervical lesions (NCCLs) are routine findings in clinical practice and are described as the loss of tooth tissue close to the cementum enamel junction. NCCLs have multifactorial etiology with main factors involved are stress, biocorrosion and friction. The different associations of these factors can lead to the weakening of the tooth structure in the cervical region, generating NCCLs. The aim of this study was to evaluate the association of etiologic factors (stress, biocorrosion and friction) on the microstructural formation of NCCLs, using finite element analysis, laser confocal microscopy and computed microtomography (micro-CT). Two three dimensional (3D), linear and elastic models of mandibular premolars were generated for stress distribution evaluation, using the finite element analysis (FEA). Two oblique loadings (50 N), were applied on the buccal cusp slope of the model. The occlusal loading (CO) was applied on the grinding region of the buccal cusp and the buccal loading (CV) on the smooth region of the same cusp. Both loads were applied at 45̊ to the tooth long axis, simulating mandibular excursive movements. The displacement restriction was applied on the base and lateral surface of the model and the results were analyzed by the maximum principal criteria. Then, for analysis by confocal microscopy, forty intact human mandibular premolars were selected and allocated randomly into four groups, according to the factor association type (n=10): (TB) stress-biocorrosion, (TF) stress-friction, (BF) biocorrosion-friction, and (TBF) stress-biocorrosion-friction. In each group, the challenges were performed in an alternate way. The stress was simulated by cyclic fatigue loading, with specimens positioned in mechanical fatigue machine and subjected to oblique load (50N), on the smooth region of the buccal cusp for 6 x 105 cycles. The biocorrosive challenge was performed using the application of hydrochloric acid (2mL/10s). The friction (abrasion) was reproduced by using electric/powered toothbrushes (300g/20s), with Colgate Total 12 toothpaste. The roughness surface, angle and height of steps were measured before and after the treatments, using a 3D laser confocal microscope. The data were analyzed using the paired t-test, two-way analysis of variance and Tukey s test (α=0.05). Then, three specimens of each group were taken to a micro-CT for the qualitative analyses. The CV loading promoted higher concentration of tensile stress in the cervical region compared to CO. The presence of biocorrosion was an important factor to roughness changes and abrasion promoted similar behavior among the groups. The TBF association promoted the greatest alterations in tooth structures. Enamel and dentin showed distinct performance according to the different interactions. The FEA results presented relationship with the experimental findings for TF,TB, TBF groups. The TBF was the more influent association on the microstructural formation of NCCLs, for enamel. For dentin, the association of at least two factors was sufficient to generate significant tissue degradation. All studied associations of etiological factors showed potential to promote NCCLs formation.As lesões cervicais não cariosas (LCNCs) são achados rotineiros na prática clínica odontológica e são descritas como a perda de tecido dentário próximo à junção amelo cementária (JAC). Essas lesões apresentam etiologia multifatorial e tem como principais fatores envolvidos nesse processo tensão, biocorrosão e fricção. As associações desses fatores levam ao enfraquecimento da estrutura dental na região cervical, promovendo o surgimento de LCNCs. O objetivo deste estudo foi avaliar o efeito das associações de fatores etiológicos (tensão, biocorrosão e fricção) na formação microestrutural de LCNCs, empregando método de elementos finitos (MEF), microscopia confocal a laser e microtomografia computadorizada (micro-CT). Dois modelos tridimensionais (3D), lineares e elásticos de pré-molares inferiores foram gerados para análise do padrão de distribuição de tensões na região cervical, pelo método de elementos finitos (MEF). Dois tipos de cargas de 50N, foram aplicadas obliquamente a cúspide vestibular do modelo. A carga oclusal (CO), foi aplicada na vertente triturante da cúspide vestibular e a carga vestibular (CV), na vertente lisa da mesma cúspide. As cargas foram posicionadas a 45º do longo do eixo do dente, e simularam interferências oclusais nos movimentos excursivos da mandíbula. A restrição do deslocamento foi realizada na base e laterais do modelo e a análise dos resultados conduzida por meio do critério de Tensão Máxima Principal. Para a análise por microscopia confocal, quarenta pré-molares inferiores humanos hígidos foram selecionados e distribuídos aleatoriamente em quatro grupos, de acordo com as seguintes associações de fatores (n=10): tensão-biocorrosão (TB), tensão-fricção (TF), e biocorrosão-fricção (BF) e tensão-biocorrosão-fricção (TBF). Para cada grupo, os desafios foram realizados de forma alternada. A tensão foi simulada por meio de ciclagem mecânica, com os espécimes posicionados em máquina de fadiga cíclica e submetidos a carregamentos oblíquos de 50N, na frequência de 2Hz, na vertente lisa e na vertente triturante da face vestibular, por 6 x 105 ciclos. Os desafios biocorrosivos foram realizados pela aplicação de 2mL de ácido hidroclorídrico por 10s. E os desafios de fricção (abrasão) foram reproduzidos com escovas elétricas (300g / 20s), com a utlização de pasta Colgate Total 12. A rugosidade da superfície, ângulo e altura dos degraus foram medidos antes e após os tratamentos, utilizando microscópio confocal a laser 3D. Para avaliação da distribuição e homogeineidade dos dados, foram realizados os testes de Shapiro Wilk e Levene. E posteriormente, aplicados o teste t-pareado, análise de variância em fator (ANOVA) e teste de Tukey (α = 0,05). Em seguida, três amostras de cada grupo foram analisadas no micro-CT para as análises qualitativas de perda estrutural. O CV promoveu maior concentração de tensões de tração na região cervical do que o CO. A presença da biocorrosão foi fator importante na alteração de rugosidade e a abrasão resultou em comportamento semelhante entre os grupos. A associação TBF promoveu as maiores alterações nas estruturas dentais. Esmalte e dentina mostraram desempenho distinto de acordo com as diferentes interações. Foi verificada relação entre os resultados gerados pelo MEF e os achados experimentais para os grupos TF, TBF, TB. O grupo TBF foi mais influente na formação microestrutural das LCNCs, para esmalte. Para dentina, a associação de pelo menos dois fatores foi suficiente para gerar degradação significativa de tecido na região cervical. Todos as associações de fatores estudadas mostraram potencial para promover a formação de LCNCs.Conselho Nacional de Desenvolvimento Científico e TecnológicoMestre em Odontologiaapplication/pdfporUniversidade Federal de UberlândiaPrograma de Pós-graduação em OdontologiaUFUBRCiências da SaúdeBiocorrosãoFadiga mecânicaFricçãoLesões cervicais não cariosasMétodo de elementos finitosMicroscopia confocalPré-molarTensãoLaser confocal microscopyNon carious cervical lesionStressBiocorrosionFrictionPremolarFinite element methodMechanical fatigueCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAssociação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSoares, Paulo Viniciushttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4774198Z4Soares, Carlos Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790296P8Raposo, Luís Henrique Araújohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4267206U0Palma-dibb, Regina Guenkahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790940Z7http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4272203Y0Zeola, Livia Fávaro81759880info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFUTHUMBNAILAssociacaoFadigaCiclica.pdf.jpgAssociacaoFadigaCiclica.pdf.jpgGenerated Thumbnailimage/jpeg1304https://repositorio.ufu.br/bitstream/123456789/17006/3/AssociacaoFadigaCiclica.pdf.jpga1f8565b68f847d3a1153f0ce94c3c60MD53ORIGINALAssociacaoFadigaCiclica.pdfapplication/pdf3999103https://repositorio.ufu.br/bitstream/123456789/17006/1/AssociacaoFadigaCiclica.pdfe13dcc7ba780c2366ae712cddbd5d586MD51TEXTAssociacaoFadigaCiclica.pdf.txtAssociacaoFadigaCiclica.pdf.txtExtracted texttext/plain106477https://repositorio.ufu.br/bitstream/123456789/17006/2/AssociacaoFadigaCiclica.pdf.txtb7c2807c8ab5588941ea3dfa25a638efMD52123456789/170062021-05-06 15:47:08.007oai:repositorio.ufu.br:123456789/17006Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2021-05-06T18:47:08Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false |
dc.title.por.fl_str_mv |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
title |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
spellingShingle |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas Zeola, Livia Fávaro Biocorrosão Fadiga mecânica Fricção Lesões cervicais não cariosas Método de elementos finitos Microscopia confocal Pré-molar Tensão Laser confocal microscopy Non carious cervical lesion Stress Biocorrosion Friction Premolar Finite element method Mechanical fatigue CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
title_full |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
title_fullStr |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
title_full_unstemmed |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
title_sort |
Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas |
author |
Zeola, Livia Fávaro |
author_facet |
Zeola, Livia Fávaro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Soares, Paulo Vinicius |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4774198Z4 |
dc.contributor.referee1.fl_str_mv |
Soares, Carlos José |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790296P8 |
dc.contributor.referee2.fl_str_mv |
Raposo, Luís Henrique Araújo |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4267206U0 |
dc.contributor.referee3.fl_str_mv |
Palma-dibb, Regina Guenka |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790940Z7 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4272203Y0 |
dc.contributor.author.fl_str_mv |
Zeola, Livia Fávaro |
contributor_str_mv |
Soares, Paulo Vinicius Soares, Carlos José Raposo, Luís Henrique Araújo Palma-dibb, Regina Guenka |
dc.subject.por.fl_str_mv |
Biocorrosão Fadiga mecânica Fricção Lesões cervicais não cariosas Método de elementos finitos Microscopia confocal Pré-molar Tensão |
topic |
Biocorrosão Fadiga mecânica Fricção Lesões cervicais não cariosas Método de elementos finitos Microscopia confocal Pré-molar Tensão Laser confocal microscopy Non carious cervical lesion Stress Biocorrosion Friction Premolar Finite element method Mechanical fatigue CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Laser confocal microscopy Non carious cervical lesion Stress Biocorrosion Friction Premolar Finite element method Mechanical fatigue |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Non carious cervical lesions (NCCLs) are routine findings in clinical practice and are described as the loss of tooth tissue close to the cementum enamel junction. NCCLs have multifactorial etiology with main factors involved are stress, biocorrosion and friction. The different associations of these factors can lead to the weakening of the tooth structure in the cervical region, generating NCCLs. The aim of this study was to evaluate the association of etiologic factors (stress, biocorrosion and friction) on the microstructural formation of NCCLs, using finite element analysis, laser confocal microscopy and computed microtomography (micro-CT). Two three dimensional (3D), linear and elastic models of mandibular premolars were generated for stress distribution evaluation, using the finite element analysis (FEA). Two oblique loadings (50 N), were applied on the buccal cusp slope of the model. The occlusal loading (CO) was applied on the grinding region of the buccal cusp and the buccal loading (CV) on the smooth region of the same cusp. Both loads were applied at 45̊ to the tooth long axis, simulating mandibular excursive movements. The displacement restriction was applied on the base and lateral surface of the model and the results were analyzed by the maximum principal criteria. Then, for analysis by confocal microscopy, forty intact human mandibular premolars were selected and allocated randomly into four groups, according to the factor association type (n=10): (TB) stress-biocorrosion, (TF) stress-friction, (BF) biocorrosion-friction, and (TBF) stress-biocorrosion-friction. In each group, the challenges were performed in an alternate way. The stress was simulated by cyclic fatigue loading, with specimens positioned in mechanical fatigue machine and subjected to oblique load (50N), on the smooth region of the buccal cusp for 6 x 105 cycles. The biocorrosive challenge was performed using the application of hydrochloric acid (2mL/10s). The friction (abrasion) was reproduced by using electric/powered toothbrushes (300g/20s), with Colgate Total 12 toothpaste. The roughness surface, angle and height of steps were measured before and after the treatments, using a 3D laser confocal microscope. The data were analyzed using the paired t-test, two-way analysis of variance and Tukey s test (α=0.05). Then, three specimens of each group were taken to a micro-CT for the qualitative analyses. The CV loading promoted higher concentration of tensile stress in the cervical region compared to CO. The presence of biocorrosion was an important factor to roughness changes and abrasion promoted similar behavior among the groups. The TBF association promoted the greatest alterations in tooth structures. Enamel and dentin showed distinct performance according to the different interactions. The FEA results presented relationship with the experimental findings for TF,TB, TBF groups. The TBF was the more influent association on the microstructural formation of NCCLs, for enamel. For dentin, the association of at least two factors was sufficient to generate significant tissue degradation. All studied associations of etiological factors showed potential to promote NCCLs formation. |
publishDate |
2015 |
dc.date.available.fl_str_mv |
2015-02-23 2016-06-22T18:47:37Z |
dc.date.issued.fl_str_mv |
2015-02-12 |
dc.date.accessioned.fl_str_mv |
2016-06-22T18:47:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ZEOLA, Livia Fávaro. Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas. 2015. 85 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Uberlândia, Uberlândia, 2015. Disponível em: https://doi.org/10.14393/ufu.di.2015.46 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufu.br/handle/123456789/17006 |
dc.identifier.doi.por.fl_str_mv |
https://doi.org/10.14393/ufu.di.2015.46 |
identifier_str_mv |
ZEOLA, Livia Fávaro. Associação de fadiga cíclica, biocorrosão e fricção na formação microestrutural de lesões cervicais não cariosas. 2015. 85 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Uberlândia, Uberlândia, 2015. Disponível em: https://doi.org/10.14393/ufu.di.2015.46 |
url |
https://repositorio.ufu.br/handle/123456789/17006 https://doi.org/10.14393/ufu.di.2015.46 |
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Universidade Federal de Uberlândia |
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Programa de Pós-graduação em Odontologia |
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UFU |
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BR |
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Ciências da Saúde |
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Universidade Federal de Uberlândia |
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