Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)

Detalhes bibliográficos
Autor(a) principal: Silva, Sheila Assunção da
Data de Publicação: 2019
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFU
Texto Completo: https://repositorio.ufu.br/handle/123456789/26335
Resumo: Eugenia calycina is a plant species found in the Cerrado, popularly known as "pitanga do cerrado". Studies showed the presence of oxygenated and non-oxygenated sesquiterpenes in the essential oil of the leaves, which showed good activity against bacteria present in the oral cavity. It is known that the composition of essential oils is subject to variations caused by climatic conditions and vegetative stages. Due to these variations, it is important to determine the best time of year for leaf collection. The objective of this work was to evaluate the influence of seasonal variations on the chemical composition of the essential oil of fresh E. calycina leaves, as well as their antibacterial activity against bacteria of the oral cavity. This study identified and compared compounds of the essential oil extracted from fresh leaves collected every two months, the first collection was in January and the last one was in November 2018. The essential oil was extracted by hydrodistillation and its chemical composition was determined by analysis using gas chromatographic coupled to mass spectrometry (GC-MS). It was observed that the chemical composition of the essential oil varied throughout the year, qualitatively and quantitatively. In sample 1 (collection and extraction performed in January) the major constituent of the essential oil was bicyclogermacrene (22.13%), while in samples 2, 3, 4 and 6 (collections and extractions performed in March, May, July and November, respectively) the major constituent was spatulenol (54.61, 15.60, 16.50, 24.04%). Sample 5 (collection and extraction performed in September) presented trans-caryophyllene as the major constituent (20.22%). Essential oil showed a homogeneous yield throughout the year, approximately 0.35%. We highlight the sample 4 that showed 0.18% yield, showing as the worst period for collection. Antibacterial activity against oral cavity bacteria (aerobic and anaerobic) was performed by microdilution in microplate broth, the result being expressed as minimum inhibitory concentration (MIC). Antibacterial activity showed that it is not influenced by rainfall index, since all samples showed moderate antimicrobial activity against aerobic bacteria (MIC between 200 to 400 μg mL -1) and for anaerobic bacteria, all samples showed low antibacterial activity (MIC equal to or greater than 400 μg mL-1).
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spelling Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)Seasonal evaluation and antibacterial activity of the essential oil of leaves of Eugenia calycina (pitanga-do-cerrado)Eugenia calycinaSazonalidadeTerpenoidesAtividade antimicrobianaEugenia calycinaSeasonalityTerpenoidsAntimicrobian activityCNPQ::CIENCIAS EXATAS E DA TERRAEugenia calycina is a plant species found in the Cerrado, popularly known as "pitanga do cerrado". Studies showed the presence of oxygenated and non-oxygenated sesquiterpenes in the essential oil of the leaves, which showed good activity against bacteria present in the oral cavity. It is known that the composition of essential oils is subject to variations caused by climatic conditions and vegetative stages. Due to these variations, it is important to determine the best time of year for leaf collection. The objective of this work was to evaluate the influence of seasonal variations on the chemical composition of the essential oil of fresh E. calycina leaves, as well as their antibacterial activity against bacteria of the oral cavity. This study identified and compared compounds of the essential oil extracted from fresh leaves collected every two months, the first collection was in January and the last one was in November 2018. The essential oil was extracted by hydrodistillation and its chemical composition was determined by analysis using gas chromatographic coupled to mass spectrometry (GC-MS). It was observed that the chemical composition of the essential oil varied throughout the year, qualitatively and quantitatively. In sample 1 (collection and extraction performed in January) the major constituent of the essential oil was bicyclogermacrene (22.13%), while in samples 2, 3, 4 and 6 (collections and extractions performed in March, May, July and November, respectively) the major constituent was spatulenol (54.61, 15.60, 16.50, 24.04%). Sample 5 (collection and extraction performed in September) presented trans-caryophyllene as the major constituent (20.22%). Essential oil showed a homogeneous yield throughout the year, approximately 0.35%. We highlight the sample 4 that showed 0.18% yield, showing as the worst period for collection. Antibacterial activity against oral cavity bacteria (aerobic and anaerobic) was performed by microdilution in microplate broth, the result being expressed as minimum inhibitory concentration (MIC). Antibacterial activity showed that it is not influenced by rainfall index, since all samples showed moderate antimicrobial activity against aerobic bacteria (MIC between 200 to 400 μg mL -1) and for anaerobic bacteria, all samples showed low antibacterial activity (MIC equal to or greater than 400 μg mL-1).Trabalho de Conclusão de Curso (Graduação)Eugenia calycina é uma espécie vegetal encontrada no Cerrado, popularmente conhecida como “pitanga do cerrado”. Trabalhos realizados revelaram a presença de sesquiterpenos oxigenados e não oxigenados no óleo essencial das folhas, que apresentou boa atividade contra bactérias encontradas na cavidade oral. Sabe-se que as composições químicas dos óleos essenciais estão sujeitas às variações provocadas pelas condições climáticas e estágios vegetativos. Devido a estas variações, se torna importante determinar o melhor período do ano para realizar a coleta das folhas. O objetivo deste trabalho foi avaliar a influência das variações sazonais na composição química do óleo essencial das folhas frescas de E. calycina, bem como sua atividade antibacteriana contra bactérias da cavidade bucal. Este estudo identificou e comparou os compostos do óleo essencial extraído das folhas frescas coletadas de dois em dois meses, a primeira coleta iniciou-se em janeiro e a última em novembro de 2018. O óleo essencial foi extraído por hidrodestilação e a sua composição química foi determinada através da análise usando cromatografia gasosa acoplada a espectrômetro de massas (CG-EM). Observou-se que a composição química do óleo essencial variou ao longo do ano, qualitativamente e quantitativamente. Na amostra 1 (coleta e extração realizado em janeiro) o constituinte majoritário do óleo essencial foi o biciclogermacreno (22,13%), enquanto nas amostras 2 ,3, 4 e 6 (coletas e extrações realizadas em março, maio, julho e novembro respectivamente) o constituinte majoritário foi o espatulenol (54,61, 15,60, 16,50 e 24,04%, respectivamente). A amostra 5 (coleta e extração realizado em setembro) apresentou o trans-cariofileno como constituinte majoritário (20,22%). O óleo essencial apresentou rendimento homogêneo ao longo do ano, aproximadamente 0,35%. Destaca-se a amostra 4 que apresentou 0,18% de rendimento, mostrando-se como o pior período para coleta. A atividade antibacteriana contra as bactérias da cavidade bucal (aeróbicas e anaeróbicas) foi realizada por microdiluição em caldo em microplacas, sendo o resultado expresso em concentração inibitória mínima (CIM). A atividade antibacteriana demonstrou não ser influenciada pelo índice pluviométrico, uma vez que todas as amostras apresentaram atividade antimicrobiana moderada contra as bactérias aeróbicas (CIM entre 200 a 400 μg mL-1) e com relação às bactérias anaeróbias, todas as amostras apresentaram baixa atividade antibacteriana (CIM igual ou maior que 400 μg mL-1).Universidade Federal de UberlândiaBrasilQuímica IndustrialSousa, Raquel Maria Ferreira dehttp://lattes.cnpq.br/6064800347452834Oliveira, Alberto deNascimento, Michelle Nauara Gomes doSilva, Sheila Assunção da2019-07-25T14:16:21Z2019-07-25T14:16:21Z2019-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfSILVA, Sheila Assunção da. Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado). 2019. 65 f. Trabalho de Conclusão de Curso (Graduação em Química Industrial) – Universidade Federal de Uberlândia, Uberlândia, 2019.https://repositorio.ufu.br/handle/123456789/26335porAttribution-NonCommercial-NoDerivs 3.0 United Stateshttp://creativecommons.org/licenses/by-nc-nd/3.0/us/info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2021-10-21T18:08:21Zoai:repositorio.ufu.br:123456789/26335Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2021-10-21T18:08:21Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
Seasonal evaluation and antibacterial activity of the essential oil of leaves of Eugenia calycina (pitanga-do-cerrado)
title Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
spellingShingle Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
Silva, Sheila Assunção da
Eugenia calycina
Sazonalidade
Terpenoides
Atividade antimicrobiana
Eugenia calycina
Seasonality
Terpenoids
Antimicrobian activity
CNPQ::CIENCIAS EXATAS E DA TERRA
title_short Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
title_full Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
title_fullStr Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
title_full_unstemmed Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
title_sort Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado)
author Silva, Sheila Assunção da
author_facet Silva, Sheila Assunção da
author_role author
dc.contributor.none.fl_str_mv Sousa, Raquel Maria Ferreira de
http://lattes.cnpq.br/6064800347452834
Oliveira, Alberto de
Nascimento, Michelle Nauara Gomes do
dc.contributor.author.fl_str_mv Silva, Sheila Assunção da
dc.subject.por.fl_str_mv Eugenia calycina
Sazonalidade
Terpenoides
Atividade antimicrobiana
Eugenia calycina
Seasonality
Terpenoids
Antimicrobian activity
CNPQ::CIENCIAS EXATAS E DA TERRA
topic Eugenia calycina
Sazonalidade
Terpenoides
Atividade antimicrobiana
Eugenia calycina
Seasonality
Terpenoids
Antimicrobian activity
CNPQ::CIENCIAS EXATAS E DA TERRA
description Eugenia calycina is a plant species found in the Cerrado, popularly known as "pitanga do cerrado". Studies showed the presence of oxygenated and non-oxygenated sesquiterpenes in the essential oil of the leaves, which showed good activity against bacteria present in the oral cavity. It is known that the composition of essential oils is subject to variations caused by climatic conditions and vegetative stages. Due to these variations, it is important to determine the best time of year for leaf collection. The objective of this work was to evaluate the influence of seasonal variations on the chemical composition of the essential oil of fresh E. calycina leaves, as well as their antibacterial activity against bacteria of the oral cavity. This study identified and compared compounds of the essential oil extracted from fresh leaves collected every two months, the first collection was in January and the last one was in November 2018. The essential oil was extracted by hydrodistillation and its chemical composition was determined by analysis using gas chromatographic coupled to mass spectrometry (GC-MS). It was observed that the chemical composition of the essential oil varied throughout the year, qualitatively and quantitatively. In sample 1 (collection and extraction performed in January) the major constituent of the essential oil was bicyclogermacrene (22.13%), while in samples 2, 3, 4 and 6 (collections and extractions performed in March, May, July and November, respectively) the major constituent was spatulenol (54.61, 15.60, 16.50, 24.04%). Sample 5 (collection and extraction performed in September) presented trans-caryophyllene as the major constituent (20.22%). Essential oil showed a homogeneous yield throughout the year, approximately 0.35%. We highlight the sample 4 that showed 0.18% yield, showing as the worst period for collection. Antibacterial activity against oral cavity bacteria (aerobic and anaerobic) was performed by microdilution in microplate broth, the result being expressed as minimum inhibitory concentration (MIC). Antibacterial activity showed that it is not influenced by rainfall index, since all samples showed moderate antimicrobial activity against aerobic bacteria (MIC between 200 to 400 μg mL -1) and for anaerobic bacteria, all samples showed low antibacterial activity (MIC equal to or greater than 400 μg mL-1).
publishDate 2019
dc.date.none.fl_str_mv 2019-07-25T14:16:21Z
2019-07-25T14:16:21Z
2019-06-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Sheila Assunção da. Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado). 2019. 65 f. Trabalho de Conclusão de Curso (Graduação em Química Industrial) – Universidade Federal de Uberlândia, Uberlândia, 2019.
https://repositorio.ufu.br/handle/123456789/26335
identifier_str_mv SILVA, Sheila Assunção da. Avaliação sazonal e atividade antibacteriana do óleo essencial das folhas de Eugenia calycina (pitanga-do-cerrado). 2019. 65 f. Trabalho de Conclusão de Curso (Graduação em Química Industrial) – Universidade Federal de Uberlândia, Uberlândia, 2019.
url https://repositorio.ufu.br/handle/123456789/26335
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 United States
http://creativecommons.org/licenses/by-nc-nd/3.0/us/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 United States
http://creativecommons.org/licenses/by-nc-nd/3.0/us/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Química Industrial
publisher.none.fl_str_mv Universidade Federal de Uberlândia
Brasil
Química Industrial
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFU
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Repositório Institucional da UFU
collection Repositório Institucional da UFU
repository.name.fl_str_mv Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv diinf@dirbi.ufu.br
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